Standard name
Human Ortholog
Description Mitochondrial inner membrane protein; exposed to the mitochondrial matrix; associates with mitochondrial ribosomes; NOT required for respiratory growth; homolog of human Letm1, a protein implicated in Wolf-Hirschhorn syndrome
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0.05 0.07 0.09 0 0 0.05 0 0.06 0.06 0.05 0 0.06 0 0.45 0.4 0.38 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0.35 0.54 0.59 0.2 0.11 0.19 0.06 0.09 0 0.16 0.14 0.12
Mitochondria 0.97 0.9 0.87 0.9 0.83 0.9 0.9 0.8 0.88 0.89 0.92 0.91 0.8 0.59 0.74 0.06 0.08 0.12 0.83 0.77 0.77 0.69 0.68 0.7
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0.11 0.1 0.09 0.13 0.09 0.08 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.07 0 0 0 0.08 0.14 0.14 0.14 0.1 0.11 0.09 0.08 0 0 0 0 0.1 0.11 0 0 0 0 0 0
Peroxisomes 0 0 0.08 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0.09 0 0 0.05
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0.07 0 0 0 0 0 0 0 0 0 0 0.11 0.17 0.08 0.13 0.13 0.14 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 5 15 9 4 2 3 3 2 4 3 0 2 4 12 5 5 6 6 9 20 18 24 27
Bud 0 6 9 8 5 15 21 25 11 22 9 13 1 1 2 1 2 1 0 8 5 9 9 6
Bud Neck 0 1 0 0 1 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0
Bud Site 0 1 0 0 0 2 5 0 1 6 3 3 0 0 0 0 4 1 – – – – – –
Cell Periphery 1 12 15 8 1 1 5 4 8 4 4 4 8 12 14 7 12 2 0 0 1 0 0 0
Cytoplasm 4 22 25 27 36 23 35 40 26 47 33 26 12 37 17 64 80 55 0 7 12 6 18 9
Endoplasmic Reticulum 1 2 2 2 2 0 4 1 6 4 1 2 1 1 5 6 7 9 0 0 0 0 3 0
Endosome 0 0 2 1 5 2 3 10 2 0 1 0 2 8 6 6 8 2 1 5 3 4 7 7
Golgi 1 16 16 15 7 0 0 0 0 3 0 3 140 307 328 28 21 28 9 42 16 81 76 71
Mitochondria 168 414 424 352 351 538 775 651 600 727 539 507 319 338 410 8 15 17 143 352 386 360 382 428
Nucleus 0 1 1 0 1 2 5 2 4 3 3 1 0 1 0 3 2 6 0 0 0 0 1 0
Nuclear Periphery 7 16 18 9 15 68 87 73 89 75 46 45 6 4 8 2 6 4 0 2 1 4 5 5
Nucleolus 13 14 11 9 32 85 124 110 65 94 55 44 11 11 6 6 20 16 2 0 2 2 4 3
Peroxisomes 1 9 41 18 11 9 5 33 19 8 3 9 3 7 8 2 14 4 5 9 45 11 9 31
SpindlePole 0 1 3 0 2 2 9 19 9 9 3 4 1 3 1 3 6 1 0 5 4 3 4 4
Vac/Vac Membrane 11 32 7 6 11 15 13 23 19 15 15 14 43 97 43 19 26 21 1 12 0 15 14 8
Unique Cell Count 174 460 487 391 421 598 863 809 682 818 589 556 397 572 552 143 199 145 172 460 505 522 563 610
Labelled Cell Count 207 552 589 464 484 764 1094 994 862 1021 718 675 549 831 860 160 229 173 172 460 505 522 563 610


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 15.8 14.7 16.2 14.6 13.6 12.4 11.6 11.7 11.6 10.8 10.2 10.9 18.7 18.2 16.8 17.1 15.4 16.9 20.2 18.9 21.6
Std Deviation (1e-4) 3.6 4.3 4.3 4.2 4.0 3.5 3.4 3.6 3.4 4.0 3.6 3.9 4.3 5.7 3.7 9.8 7.6 8.2 5.8 5.7 6.0
Intensity Change (Log2) – – – -0.15 -0.25 -0.38 -0.49 -0.46 -0.49 -0.59 -0.67 -0.58 0.21 0.17 0.05 0.07 -0.07 0.06 0.32 0.22 0.41


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.7 -2.2 -3.6 -4.2 -4.0 -3.9 -3.8 -3.3 -4.2 -2.8 -2.9 -0.9 0 -0.4 0
Bud 0.2 -0.8 0.7 0.7 1.4 -0.3 1.0 -0.4 0.5 0 0 -2.3 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.0 -3.2 -4.0 -3.7 -3.8 -2.3 -3.8 -3.0 -2.8 -1.0 -1.0 -0.5 0 1.8 0
Cytoplasm 1.1 2.1 -1.0 -0.9 -0.2 -1.1 0.5 0.3 -0.3 -1.6 0.9 -1.7 12.0 11.6 10.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.4 -1.6 -4.5 -5.4 -5.2 -4.8 -4.3 -4.4 -3.3 12.4 17.7 19.2 6.7 3.8 6.7
Mitochondria 1.4 -1.6 1.5 1.5 -3.1 0.5 1.0 2.4 2.1 -2.7 -10.1 -5.2 -18.5 -19.7 -17.3
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -1.2 -0.1 4.7 4.2 3.6 5.5 3.7 2.8 3.0 -2.0 -3.4 -2.3 0 -0.4 -0.5
Nucleolus 0 3.8 6.9 7.1 6.8 5.0 5.9 4.8 4.1 0.5 -0.4 -1.5 0 4.5 4.6
Peroxisomes -2.2 -3.8 -5.4 -7.6 -3.3 -4.3 -6.8 -6.5 -5.1 -5.2 -5.6 -5.3 -2.9 -0.6 -2.3
SpindlePole 0 0 0 0 2.3 0 0 0 0 0 0 0 0 0 0
Vacuole 0.1 1.3 1.2 0.1 1.6 1.5 0.5 1.3 1.2 6.0 8.5 4.8 6.3 6.5 6.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 20.333 21.206 22.1182 19.6311 20.1894 20.5023 18.6336 22.7407 20.9145 18.2109 20.48 20.5402 20.4849 21.8847 21.7125 21.1313 19.9088 21.1026
Actin 0.0167 0.0083 0.008 0.0011 0.0004 0.0008 0.0079 0.0032 0.0121 0.0315 0.0075 0.0007 0.0076 0.0013 0.0011 0.0035 0.0033 0.0003
Bud 0.0002 0.0005 0.0001 0.0001 0 0 0.0007 0 0.0002 0.0002 0.0001 0 0.0001 0 0 0 0 0
Bud Neck 0 0.0001 0.0004 0 0 0 0.0004 0 0.0007 0.0005 0.0001 0 0.0001 0 0 0 0.0003 0
Bud Periphery 0.0008 0.0008 0.0002 0.0001 0 0.0001 0.0009 0.0001 0.0004 0.0004 0.0001 0 0.0001 0 0.0001 0 0 0
Bud Site 0.0003 0.0012 0.0039 0.0001 0 0 0.0025 0.0001 0.0054 0.001 0.0005 0 0.0005 0 0 0 0.0001 0
Cell Periphery 0.0006 0.0007 0.0004 0.0001 0 0 0.0005 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0 0
Cytoplasm 0.0001 0.0001 0.0002 0 0 0 0.0012 0 0.0005 0.0012 0.0055 0 0 0 0 0 0.0005 0
Cytoplasmic Foci 0.0012 0.0012 0.0011 0.0007 0.0002 0.0003 0.0088 0.0005 0.0029 0.0057 0.0045 0.0001 0.0003 0.0007 0 0.0001 0.0007 0
Eisosomes 0.0004 0.0011 0.0008 0.0001 0.0001 0.0001 0.0007 0.0003 0.0006 0.001 0.0003 0.0003 0.0006 0.0003 0.0004 0.0001 0.0002 0.0003
Endoplasmic Reticulum 0.0001 0.0001 0.0002 0 0 0 0.0012 0.0001 0.0012 0.0017 0.0034 0.0001 0 0 0 0 0.001 0
Endosome 0.0017 0.0092 0.0031 0.0021 0.0009 0.0024 0.0133 0.0027 0.0033 0.0056 0.0034 0.0012 0.0011 0.0026 0.0004 0.0008 0.005 0.0005
Golgi 0.0059 0.0353 0.0119 0.0034 0.0173 0.0066 0.0081 0.0163 0.01 0.0181 0.005 0.0048 0.0042 0.0197 0.0046 0.0011 0.0028 0.013
Lipid Particles 0.0191 0.0053 0.0008 0.0138 0.0005 0.0024 0.0164 0.0098 0.0046 0.008 0.0149 0.0071 0.0024 0.003 0.0027 0.0017 0.0013 0.0005
Mitochondria 0.9022 0.9011 0.9531 0.9585 0.9785 0.9737 0.8989 0.9522 0.952 0.9 0.9399 0.9661 0.9776 0.9673 0.9875 0.9838 0.9623 0.982
None 0.0002 0.0001 0.0001 0 0 0 0.0015 0 0.0006 0.0022 0.003 0 0.0001 0 0 0 0.001 0
Nuclear Periphery 0.0013 0.0001 0.0001 0 0 0.0001 0.0058 0 0.0003 0.004 0.0025 0.0092 0.0001 0 0.0001 0 0.0121 0
Nucleolus 0.0153 0.001 0.0001 0.0001 0 0 0.002 0.0001 0.0005 0.0006 0.0002 0.0001 0.0001 0.0001 0 0 0.0004 0
Nucleus 0.0037 0.0004 0.0001 0 0 0 0.0018 0 0.0001 0.0011 0.0007 0.0001 0 0 0 0 0.0022 0
Peroxisomes 0.0013 0.0029 0.0009 0.0031 0.0011 0.0011 0.0076 0.0031 0.0019 0.0119 0.0015 0.0004 0.0013 0.0028 0.0006 0.0018 0.0005 0.0003
Punctate Nuclear 0.0017 0 0 0 0 0 0.0046 0 0.0002 0.0032 0.0048 0.0001 0 0 0 0 0.0051 0
Vacuole 0.0047 0.0089 0.0031 0.0014 0.0001 0.0029 0.0032 0.0014 0.0004 0.0008 0.0005 0.0004 0.001 0.0004 0.0002 0.0011 0.0003 0.0002
Vacuole Periphery 0.0224 0.0217 0.0113 0.0153 0.0006 0.0094 0.0118 0.0097 0.0019 0.0014 0.0012 0.0093 0.0028 0.0016 0.0022 0.0057 0.001 0.003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 38.801 21.6786 27.347 31.7728 31.442 33.9321 24.4764 22.191 29.3449 31.7594
Translational Efficiency 1.6136 2.1697 1.6818 1.5078 1.4106 1.7379 1.5913 1.512 1.5092 1.4247

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Mitochondrial inner membrane protein; exposed to the mitochondrial matrix; associates with mitochondrial ribosomes; NOT required for respiratory growth; homolog of human Letm1, a protein implicated in Wolf-Hirschhorn syndrome
Localization
Cell Percentages mitochondrion (100%)
Cell Cycle Regulation No
Subcompartmental Group mito-3

Ylh47

Ylh47


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ylh47-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available