Standard name | |
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Human Ortholog | |
Description | Chromatin-associated protein; required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 4 | 1 | 4 | 2 | 2 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 2 | 3 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 1 | 0 | 5 | 2 | 13 | 20 | 14 | 15 | 21 | 0 | 0 | 4 | 0 | 1 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 5 | 1 | 4 | 7 | 11 | 20 | 11 | 19 | 11 | 13 | 7 | 13 | 6 | 44 | 56 | 35 | 18 | 27 | 0 | 0 | 0 | 2 | 3 | 1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 11 | 4 | 7 | 0 | 0 | 0 | 0 | 1 | 1 |
Endosome | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 3 | 6 | 2 | 2 | 2 | 10 | 1 | 0 | 2 | 0 | 1 | 3 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Mitochondria | 10 | 10 | 58 | 136 | 118 | 139 | 213 | 242 | 251 | 316 | 207 | 258 | 13 | 9 | 47 | 19 | 8 | 39 | 0 | 2 | 2 | 1 | 3 | 5 |
Nucleus | 102 | 40 | 79 | 197 | 266 | 229 | 386 | 499 | 378 | 415 | 271 | 335 | 112 | 175 | 168 | 440 | 206 | 365 | 91 | 54 | 70 | 111 | 198 | 202 |
Nuclear Periphery | 0 | 1 | 0 | 2 | 5 | 4 | 5 | 6 | 11 | 13 | 10 | 11 | 2 | 5 | 3 | 3 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 12 | 1 | 3 | 7 | 20 | 11 | 31 | 57 | 62 | 49 | 27 | 58 | 35 | 69 | 84 | 10 | 7 | 16 | 10 | 1 | 7 | 0 | 5 | 9 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 0 |
SpindlePole | 1 | 0 | 1 | 1 | 5 | 2 | 2 | 5 | 3 | 0 | 1 | 1 | 1 | 6 | 11 | 2 | 5 | 6 | 1 | 0 | 3 | 0 | 3 | 3 |
Vac/Vac Membrane | 4 | 2 | 0 | 1 | 5 | 5 | 5 | 6 | 1 | 5 | 1 | 5 | 4 | 6 | 4 | 27 | 11 | 24 | 5 | 2 | 10 | 7 | 29 | 45 |
Unique Cell Count | 117 | 45 | 94 | 224 | 335 | 295 | 467 | 642 | 464 | 529 | 348 | 428 | 146 | 287 | 305 | 511 | 244 | 441 | 118 | 64 | 100 | 133 | 255 | 283 |
Labelled Cell Count | 134 | 55 | 145 | 352 | 432 | 418 | 657 | 855 | 738 | 829 | 541 | 704 | 177 | 322 | 381 | 550 | 263 | 499 | 118 | 64 | 100 | 133 | 255 | 283 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.5 | 4.8 | 3.6 | 3.7 | 4.1 | 4.0 | 4.0 | 4.2 | 3.7 | 3.7 | 3.8 | 3.4 | 5.9 | 6.1 | 5.1 | 6.7 | 7.0 | 7.5 | 5.3 | 5.3 | 5.9 |
Std Deviation (1e-4) | 0.8 | 0.6 | 0.9 | 0.6 | 1.0 | 1.5 | 1.0 | 1.0 | 1.0 | 0.9 | 1.0 | 0.9 | 1.5 | 1.9 | 1.5 | 1.3 | 1.4 | 1.6 | 1.4 | 1.3 | 1.5 |
Intensity Change (Log2) | – | – | – | 0.02 | 0.18 | 0.14 | 0.14 | 0.2 | 0.03 | 0.02 | 0.06 | -0.08 | 0.69 | 0.74 | 0.49 | 0.88 | 0.94 | 1.04 | 0.54 | 0.53 | 0.69 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.0102 | 3.4303 | 3.1101 | 2.4482 | 2.8433 | 2.6324 | 3.1317 | 3.8257 | 3.5848 | 3.4462 | 3.2549 | 3.415 | 1.3944 | 1.9439 | 1.8516 | 1.7322 | 1.2447 | 1.3421 |
Actin | 0.0278 | 0.0006 | 0.0358 | 0.0118 | 0.001 | 0.0011 | 0.0186 | 0 | 0.0088 | 0.0029 | 0.003 | 0.0011 | 0.0043 | 0.0004 | 0.0009 | 0 | 0.011 | 0 |
Bud | 0.0004 | 0.0002 | 0.0006 | 0.0011 | 0.001 | 0.0001 | 0.0004 | 0 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0003 | 0 |
Bud Neck | 0.0016 | 0.0012 | 0.0007 | 0.0009 | 0.002 | 0.0022 | 0.002 | 0.0001 | 0.0008 | 0.0002 | 0.0001 | 0.0007 | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0.0008 | 0.0005 |
Bud Periphery | 0.0018 | 0.0003 | 0.001 | 0.0043 | 0.0028 | 0.0006 | 0.0007 | 0 | 0.0006 | 0.0009 | 0.0004 | 0.0002 | 0.0004 | 0.0002 | 0.0001 | 0 | 0.0004 | 0 |
Bud Site | 0.0009 | 0.0007 | 0.0073 | 0.0027 | 0.0013 | 0.0002 | 0.0018 | 0.0001 | 0.0016 | 0.0002 | 0.0002 | 0.0001 | 0.0009 | 0.0003 | 0.0006 | 0 | 0.0011 | 0 |
Cell Periphery | 0.003 | 0.0006 | 0.0003 | 0.0009 | 0.0006 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 | 0 |
Cytoplasm | 0.0022 | 0.0008 | 0.0009 | 0.0028 | 0.0057 | 0.0002 | 0.0089 | 0.0001 | 0.0027 | 0.0002 | 0.0005 | 0.0002 | 0.0021 | 0.0003 | 0.0001 | 0.0008 | 0.0011 | 0 |
Cytoplasmic Foci | 0.0049 | 0.0001 | 0.0014 | 0.0119 | 0.001 | 0.0001 | 0.0072 | 0 | 0.0118 | 0.0014 | 0.0032 | 0.0006 | 0.0083 | 0 | 0.0033 | 0 | 0.0014 | 0 |
Eisosomes | 0.0003 | 0 | 0.0002 | 0.0003 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 | 0.0003 | 0 |
Endoplasmic Reticulum | 0.0034 | 0.0001 | 0.0017 | 0.01 | 0.0031 | 0.0022 | 0.0033 | 0 | 0.0014 | 0.001 | 0.0014 | 0.0001 | 0.002 | 0.0003 | 0.0001 | 0.0001 | 0.0004 | 0 |
Endosome | 0.0228 | 0.0002 | 0.0053 | 0.0176 | 0.012 | 0.0044 | 0.0154 | 0 | 0.0149 | 0.0146 | 0.0164 | 0.0015 | 0.0165 | 0.0011 | 0.0041 | 0.0002 | 0.0021 | 0 |
Golgi | 0.0078 | 0.0001 | 0.0028 | 0.0057 | 0.0018 | 0.0033 | 0.0031 | 0 | 0.0034 | 0.0066 | 0.0044 | 0.003 | 0.0036 | 0.0001 | 0.0048 | 0 | 0.0048 | 0 |
Lipid Particles | 0.02 | 0.0001 | 0.0033 | 0.029 | 0.002 | 0.0009 | 0.0162 | 0 | 0.0039 | 0.0108 | 0.0103 | 0.0005 | 0.027 | 0.0001 | 0.0029 | 0 | 0.0001 | 0 |
Mitochondria | 0.051 | 0.0013 | 0.0118 | 0.024 | 0.093 | 0.0063 | 0.0063 | 0.0003 | 0.0089 | 0.0234 | 0.0755 | 0.0016 | 0.0164 | 0.0031 | 0.0058 | 0.0002 | 0.023 | 0.0001 |
None | 0.0011 | 0.0001 | 0.0005 | 0.0055 | 0.0004 | 0.0001 | 0.0052 | 0 | 0.0006 | 0.0003 | 0.0003 | 0.0001 | 0.0066 | 0.0004 | 0.0001 | 0.0003 | 0.0002 | 0 |
Nuclear Periphery | 0.027 | 0.0082 | 0.0175 | 0.0106 | 0.0385 | 0.0145 | 0.038 | 0.0004 | 0.0124 | 0.0137 | 0.0042 | 0.0018 | 0.0109 | 0.0419 | 0.0138 | 0.0102 | 0.015 | 0.0002 |
Nucleolus | 0.0327 | 0.0625 | 0.0571 | 0.0142 | 0.0189 | 0.0569 | 0.02 | 0.0215 | 0.0465 | 0.0036 | 0.0101 | 0.0243 | 0.0254 | 0.0232 | 0.0501 | 0.004 | 0.0196 | 0.0375 |
Nucleus | 0.7644 | 0.9135 | 0.8375 | 0.8095 | 0.7979 | 0.9024 | 0.8128 | 0.9749 | 0.8675 | 0.8979 | 0.865 | 0.9615 | 0.8439 | 0.889 | 0.8865 | 0.9832 | 0.9106 | 0.9609 |
Peroxisomes | 0.0051 | 0.0001 | 0.0033 | 0.0221 | 0.0015 | 0.0001 | 0.0084 | 0 | 0.0034 | 0.001 | 0.0014 | 0.0003 | 0.0035 | 0.0001 | 0.0054 | 0 | 0.0004 | 0 |
Punctate Nuclear | 0.0094 | 0.0055 | 0.004 | 0.003 | 0.0018 | 0.0012 | 0.0238 | 0.0024 | 0.0076 | 0.0013 | 0.0016 | 0.0021 | 0.0217 | 0.0371 | 0.0202 | 0.0004 | 0.0066 | 0.0007 |
Vacuole | 0.0069 | 0.0025 | 0.0044 | 0.0083 | 0.01 | 0.0012 | 0.0041 | 0.0001 | 0.0017 | 0.0097 | 0.0007 | 0.0002 | 0.0033 | 0.0007 | 0.0004 | 0.0004 | 0.0003 | 0.0001 |
Vacuole Periphery | 0.0055 | 0.0011 | 0.0027 | 0.0039 | 0.0038 | 0.0016 | 0.0035 | 0.0001 | 0.0009 | 0.0098 | 0.001 | 0.0001 | 0.0021 | 0.0013 | 0.0004 | 0.0002 | 0.0002 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 40.6246 | 12.0463 | 7.8872 | 16.4863 | 25.4365 | 34.8584 | 12.2695 | 8.8251 | 14.0723 | 33.8194 |
Translational Efficiency | 1.2672 | 0.9165 | 1.0375 | 0.9332 | 1.1206 | 1.3344 | 0.7472 | 0.6016 | 0.7833 | 0.8931 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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