Standard name
Human Ortholog
Description Chromatin-associated protein; required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0.15 0.18 0.07 0.07 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.09 0.22 0.62 0.61 0.35 0.47 0.46 0.38 0.54 0.6 0.59 0.6 0.09 0 0.15 0 0 0.09 0 0 0 0 0 0
Nucleus 0.87 0.89 0.84 0.88 0.79 0.78 0.83 0.78 0.81 0.78 0.78 0.78 0.77 0.61 0.55 0.86 0.84 0.83 0.78 0.85 0.7 0.84 0.78 0.71
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.1 0 0 0 0.06 0 0.07 0.09 0.13 0.09 0.08 0.14 0.24 0.24 0.28 0 0 0 0.09 0 0.07 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.05 0 0 0.11 0.06 0.12 0.16
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 3 1 4 1 4 2 2 0 1 0 1 0 0 1 0 0 2 3 3
Bud Neck 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 1 0 5 2 13 20 14 15 21 0 0 4 0 1 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 5 1 4 7 11 20 11 19 11 13 7 13 6 44 56 35 18 27 0 0 0 2 3 1
Endoplasmic Reticulum 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 11 4 7 0 0 0 0 1 1
Endosome 0 0 0 0 2 0 1 1 0 0 0 0 3 6 2 2 2 10 1 0 2 0 1 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Mitochondria 10 10 58 136 118 139 213 242 251 316 207 258 13 9 47 19 8 39 0 2 2 1 3 5
Nucleus 102 40 79 197 266 229 386 499 378 415 271 335 112 175 168 440 206 365 91 54 70 111 198 202
Nuclear Periphery 0 1 0 2 5 4 5 6 11 13 10 11 2 5 3 3 1 3 0 0 0 0 0 0
Nucleolus 12 1 3 7 20 11 31 57 62 49 27 58 35 69 84 10 7 16 10 1 7 0 5 9
Peroxisomes 0 0 0 0 0 0 0 1 0 0 0 0 1 1 1 0 0 2 0 0 0 1 0 0
SpindlePole 1 0 1 1 5 2 2 5 3 0 1 1 1 6 11 2 5 6 1 0 3 0 3 3
Vac/Vac Membrane 4 2 0 1 5 5 5 6 1 5 1 5 4 6 4 27 11 24 5 2 10 7 29 45
Unique Cell Count 117 45 94 224 335 295 467 642 464 529 348 428 146 287 305 511 244 441 118 64 100 133 255 283
Labelled Cell Count 134 55 145 352 432 418 657 855 738 829 541 704 177 322 381 550 263 499 118 64 100 133 255 283


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 4.8 3.6 3.7 4.1 4.0 4.0 4.2 3.7 3.7 3.8 3.4 5.9 6.1 5.1 6.7 7.0 7.5 5.3 5.3 5.9
Std Deviation (1e-4) 0.8 0.6 0.9 0.6 1.0 1.5 1.0 1.0 1.0 0.9 1.0 0.9 1.5 1.9 1.5 1.3 1.4 1.6 1.4 1.3 1.5
Intensity Change (Log2) 0.02 0.18 0.14 0.14 0.2 0.03 0.02 0.06 -0.08 0.69 0.74 0.49 0.88 0.94 1.04 0.54 0.53 0.69


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.0102 3.4303 3.1101 2.4482 2.8433 2.6324 3.1317 3.8257 3.5848 3.4462 3.2549 3.415 1.3944 1.9439 1.8516 1.7322 1.2447 1.3421
Actin 0.0278 0.0006 0.0358 0.0118 0.001 0.0011 0.0186 0 0.0088 0.0029 0.003 0.0011 0.0043 0.0004 0.0009 0 0.011 0
Bud 0.0004 0.0002 0.0006 0.0011 0.001 0.0001 0.0004 0 0.0003 0.0003 0.0001 0.0001 0.0002 0.0001 0.0001 0 0.0003 0
Bud Neck 0.0016 0.0012 0.0007 0.0009 0.002 0.0022 0.002 0.0001 0.0008 0.0002 0.0001 0.0007 0.0004 0.0002 0.0002 0.0001 0.0008 0.0005
Bud Periphery 0.0018 0.0003 0.001 0.0043 0.0028 0.0006 0.0007 0 0.0006 0.0009 0.0004 0.0002 0.0004 0.0002 0.0001 0 0.0004 0
Bud Site 0.0009 0.0007 0.0073 0.0027 0.0013 0.0002 0.0018 0.0001 0.0016 0.0002 0.0002 0.0001 0.0009 0.0003 0.0006 0 0.0011 0
Cell Periphery 0.003 0.0006 0.0003 0.0009 0.0006 0.0001 0.0002 0 0.0001 0.0002 0.0001 0 0.0002 0.0001 0 0 0.0001 0
Cytoplasm 0.0022 0.0008 0.0009 0.0028 0.0057 0.0002 0.0089 0.0001 0.0027 0.0002 0.0005 0.0002 0.0021 0.0003 0.0001 0.0008 0.0011 0
Cytoplasmic Foci 0.0049 0.0001 0.0014 0.0119 0.001 0.0001 0.0072 0 0.0118 0.0014 0.0032 0.0006 0.0083 0 0.0033 0 0.0014 0
Eisosomes 0.0003 0 0.0002 0.0003 0.0001 0 0.0001 0 0.0002 0 0.0001 0 0.0002 0 0 0 0.0003 0
Endoplasmic Reticulum 0.0034 0.0001 0.0017 0.01 0.0031 0.0022 0.0033 0 0.0014 0.001 0.0014 0.0001 0.002 0.0003 0.0001 0.0001 0.0004 0
Endosome 0.0228 0.0002 0.0053 0.0176 0.012 0.0044 0.0154 0 0.0149 0.0146 0.0164 0.0015 0.0165 0.0011 0.0041 0.0002 0.0021 0
Golgi 0.0078 0.0001 0.0028 0.0057 0.0018 0.0033 0.0031 0 0.0034 0.0066 0.0044 0.003 0.0036 0.0001 0.0048 0 0.0048 0
Lipid Particles 0.02 0.0001 0.0033 0.029 0.002 0.0009 0.0162 0 0.0039 0.0108 0.0103 0.0005 0.027 0.0001 0.0029 0 0.0001 0
Mitochondria 0.051 0.0013 0.0118 0.024 0.093 0.0063 0.0063 0.0003 0.0089 0.0234 0.0755 0.0016 0.0164 0.0031 0.0058 0.0002 0.023 0.0001
None 0.0011 0.0001 0.0005 0.0055 0.0004 0.0001 0.0052 0 0.0006 0.0003 0.0003 0.0001 0.0066 0.0004 0.0001 0.0003 0.0002 0
Nuclear Periphery 0.027 0.0082 0.0175 0.0106 0.0385 0.0145 0.038 0.0004 0.0124 0.0137 0.0042 0.0018 0.0109 0.0419 0.0138 0.0102 0.015 0.0002
Nucleolus 0.0327 0.0625 0.0571 0.0142 0.0189 0.0569 0.02 0.0215 0.0465 0.0036 0.0101 0.0243 0.0254 0.0232 0.0501 0.004 0.0196 0.0375
Nucleus 0.7644 0.9135 0.8375 0.8095 0.7979 0.9024 0.8128 0.9749 0.8675 0.8979 0.865 0.9615 0.8439 0.889 0.8865 0.9832 0.9106 0.9609
Peroxisomes 0.0051 0.0001 0.0033 0.0221 0.0015 0.0001 0.0084 0 0.0034 0.001 0.0014 0.0003 0.0035 0.0001 0.0054 0 0.0004 0
Punctate Nuclear 0.0094 0.0055 0.004 0.003 0.0018 0.0012 0.0238 0.0024 0.0076 0.0013 0.0016 0.0021 0.0217 0.0371 0.0202 0.0004 0.0066 0.0007
Vacuole 0.0069 0.0025 0.0044 0.0083 0.01 0.0012 0.0041 0.0001 0.0017 0.0097 0.0007 0.0002 0.0033 0.0007 0.0004 0.0004 0.0003 0.0001
Vacuole Periphery 0.0055 0.0011 0.0027 0.0039 0.0038 0.0016 0.0035 0.0001 0.0009 0.0098 0.001 0.0001 0.0021 0.0013 0.0004 0.0002 0.0002 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 40.6246 12.0463 7.8872 16.4863 25.4365 34.8584 12.2695 8.8251 14.0723 33.8194
Translational Efficiency 1.2672 0.9165 1.0375 0.9332 1.1206 1.3344 0.7472 0.6016 0.7833 0.8931

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Chromatin-associated protein; required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion
Localization
Cell Percentages nucleus (80%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Ctf4

Ctf4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ctf4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available