Standard name | |
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Human Ortholog | |
Description | Minus-end-directed microtubule motor; functions in mitosis and meiosis, localizes to the spindle pole body and localization is dependent on functional Cik1p, required for nuclear fusion during mating; potential Cdc28p substrate |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 0 | 2 | 2 | 2 | 3 | 1 | – | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 21 | 36 | 42 |
Bud | 0 | 2 | 4 | 7 | 12 | 16 | 21 | – | 20 | 15 | 33 | 13 | 1 | 0 | 0 | 4 | 1 | 5 | 12 | 9 | 10 | 24 | 22 | 28 |
Bud Neck | 0 | 0 | 12 | 1 | 19 | 6 | 16 | – | 9 | 10 | 11 | 12 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 2 | 4 | 5 | 8 | 9 |
Bud Site | 3 | 2 | 2 | 8 | 12 | 11 | 19 | – | 23 | 17 | 25 | 19 | 4 | 0 | 1 | 2 | 0 | 2 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | 27 | 31 | 62 | 52 | 52 | 57 | 86 | – | 60 | 60 | 47 | 75 | 54 | 103 | 99 | 41 | 22 | 24 | 12 | 15 | 18 | 14 | 23 | 24 |
Endoplasmic Reticulum | 0 | 1 | 2 | 0 | 1 | 0 | 2 | – | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 12 | 7 | 4 | 0 | 0 | 0 | 5 | 10 | 5 |
Endosome | 35 | 45 | 13 | 12 | 9 | 32 | 10 | – | 7 | 3 | 3 | 17 | 49 | 68 | 47 | 55 | 55 | 32 | 41 | 28 | 37 | 27 | 32 | 37 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 0 | 4 | 4 |
Mitochondria | 66 | 45 | 12 | 22 | 25 | 97 | 159 | – | 201 | 208 | 161 | 147 | 28 | 13 | 8 | 44 | 13 | 14 | 7 | 11 | 19 | 23 | 15 | 6 |
Nucleus | 98 | 68 | 54 | 48 | 41 | 92 | 94 | – | 65 | 71 | 50 | 53 | 13 | 13 | 14 | 45 | 27 | 37 | 83 | 75 | 51 | 2 | 0 | 0 |
Nuclear Periphery | 3 | 2 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 1 | 0 | 2 | 0 | 1 | 1 | 1 | 2 | 1 | 1 | 1 | 0 | 0 | 0 |
Nucleolus | 120 | 60 | 44 | 39 | 69 | 59 | 70 | – | 42 | 46 | 58 | 43 | 75 | 102 | 93 | 117 | 75 | 80 | 14 | 15 | 11 | 0 | 0 | 0 |
Peroxisomes | 1 | 5 | 1 | 0 | 1 | 4 | 0 | – | 5 | 0 | 1 | 2 | 4 | 8 | 10 | 0 | 2 | 3 | 4 | 1 | 3 | 9 | 8 | 7 |
SpindlePole | 121 | 74 | 159 | 141 | 231 | 171 | 161 | – | 89 | 81 | 94 | 118 | 63 | 63 | 53 | 118 | 88 | 91 | 228 | 143 | 204 | 48 | 48 | 52 |
Vac/Vac Membrane | 35 | 49 | 31 | 5 | 16 | 16 | 15 | – | 10 | 23 | 11 | 16 | 27 | 20 | 17 | 29 | 19 | 18 | 25 | 22 | 33 | 10 | 6 | 2 |
Unique Cell Count | 361 | 272 | 317 | 259 | 370 | 423 | 468 | 374 | 363 | 329 | 378 | 242 | 284 | 262 | 301 | 196 | 198 | 449 | 339 | 413 | 202 | 227 | 233 | |
Labelled Cell Count | 510 | 384 | 399 | 337 | 490 | 564 | 654 | 533 | 535 | 495 | 517 | 321 | 390 | 343 | 469 | 310 | 314 | 449 | 339 | 413 | 202 | 227 | 233 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.0 | 4.9 | 5.4 | 4.9 | 5.5 | 4.5 | 4.5 | – | 3.9 | 3.9 | 3.6 | 4.2 | 4.5 | 4.9 | 4.7 | 7.2 | 7.7 | 7.0 | 6.3 | 7.7 | 9.4 |
Std Deviation (1e-4) | 0.7 | 0.9 | 0.9 | 1.3 | 1.3 | 1.1 | 1.3 | – | 1.0 | 1.0 | 0.8 | 1.1 | 0.8 | 1.6 | 1.2 | 1.6 | 1.6 | 1.5 | 1.9 | 2.2 | 2.4 |
Intensity Change (Log2) | – | – | – | -0.13 | 0.03 | -0.27 | -0.25 | – | -0.46 | -0.47 | -0.56 | -0.35 | -0.24 | -0.14 | -0.21 | 0.43 | 0.51 | 0.38 | 0.23 | 0.52 | 0.81 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | -0.1 | 0 | -0.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.374 | 0.4391 | 0.444 | 0.6338 | 0.3171 | 0.135 | 1.1807 | 1.1668 | 1.3609 | 1.3667 | 0.7456 | 0.903 | 0.7104 | 0.6997 | 0.8574 | 1.0332 | 1.4826 | 0.2944 |
Actin | 0.0007 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0005 | 0.011 | 0.0001 | 0.0052 | 0.009 | 0.0017 | 0.0007 | 0.0357 | 0.0002 | 0.0062 | 0.0001 | 0.0348 | 0.0037 |
Bud | 0.0001 | 0.0003 | 0.0001 | 0 | 0 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0004 | 0.0002 | 0 | 0.0004 | 0.0001 |
Bud Neck | 0.0013 | 0.0032 | 0.0076 | 0.0047 | 0.0008 | 0.0062 | 0.003 | 0.0011 | 0.003 | 0.0235 | 0.0011 | 0.0061 | 0.0014 | 0.0025 | 0.0095 | 0.0012 | 0.0015 | 0.012 |
Bud Periphery | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0.0002 | 0.0006 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0006 | 0.0002 | 0.0003 | 0 | 0.0013 | 0.0001 |
Bud Site | 0.0013 | 0.0034 | 0.0007 | 0.0003 | 0.0001 | 0.0002 | 0.0054 | 0.004 | 0.0009 | 0.0006 | 0.0002 | 0.0002 | 0.0009 | 0.0121 | 0.0075 | 0.0004 | 0.0004 | 0.0005 |
Cell Periphery | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.0007 | 0 | 0.0001 | 0 |
Cytoplasm | 0.0347 | 0.0497 | 0.0049 | 0.0012 | 0.0002 | 0.0147 | 0.0303 | 0.0231 | 0.0021 | 0.0003 | 0.0005 | 0.0127 | 0.0372 | 0.0397 | 0.0131 | 0.0002 | 0.0019 | 0.0221 |
Cytoplasmic Foci | 0.0267 | 0.0061 | 0.0126 | 0.0119 | 0.02 | 0.0073 | 0.0242 | 0.0024 | 0.0089 | 0.014 | 0.0035 | 0.0035 | 0.0629 | 0.0162 | 0.0157 | 0.0257 | 0.0175 | 0.0036 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0.0009 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0022 | 0.0026 | 0.0002 | 0.0001 | 0 | 0.0009 | 0.0041 | 0.0012 | 0.0004 | 0.0001 | 0.0003 | 0.0003 | 0.0049 | 0.0009 | 0.0027 | 0 | 0.0045 | 0.0006 |
Endosome | 0.0054 | 0.0029 | 0.0013 | 0.0025 | 0.0015 | 0.0019 | 0.0135 | 0.0012 | 0.0042 | 0.002 | 0.0029 | 0.0021 | 0.0237 | 0.0014 | 0.0032 | 0.0008 | 0.0213 | 0.0027 |
Golgi | 0.0007 | 0.0002 | 0.0003 | 0.0004 | 0.0011 | 0.0002 | 0.0032 | 0.0001 | 0.002 | 0.0018 | 0.0024 | 0.0003 | 0.0075 | 0.0002 | 0.0009 | 0.0005 | 0.0172 | 0.0005 |
Lipid Particles | 0.0089 | 0.0018 | 0.0033 | 0.0042 | 0.0161 | 0.0036 | 0.0228 | 0.0004 | 0.0095 | 0.0124 | 0.0201 | 0.0013 | 0.017 | 0.0012 | 0.0027 | 0.0037 | 0.0209 | 0.0012 |
Mitochondria | 0.0006 | 0.0004 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0045 | 0.0002 | 0.0009 | 0.0139 | 0.0017 | 0.0002 | 0.0023 | 0.0004 | 0.0004 | 0 | 0.0044 | 0.0002 |
None | 0.0839 | 0.073 | 0.0037 | 0.0008 | 0.0002 | 0.0327 | 0.055 | 0.0298 | 0.006 | 0.0003 | 0.0006 | 0.0144 | 0.1713 | 0.1003 | 0.0081 | 0.0002 | 0.0014 | 0.0507 |
Nuclear Periphery | 0.0181 | 0.0179 | 0.0055 | 0.0021 | 0.0018 | 0.007 | 0.0395 | 0.0116 | 0.0055 | 0.0048 | 0.0334 | 0.009 | 0.0332 | 0.0086 | 0.0042 | 0.002 | 0.048 | 0.0071 |
Nucleolus | 0.0218 | 0.0477 | 0.0358 | 0.0311 | 0.0294 | 0.031 | 0.019 | 0.0506 | 0.0401 | 0.011 | 0.04 | 0.0353 | 0.0056 | 0.0405 | 0.0733 | 0.0333 | 0.0243 | 0.0393 |
Nucleus | 0.2523 | 0.3694 | 0.0981 | 0.0789 | 0.0139 | 0.3882 | 0.1876 | 0.2769 | 0.0517 | 0.006 | 0.0266 | 0.3251 | 0.1326 | 0.3181 | 0.0729 | 0.038 | 0.0146 | 0.4084 |
Peroxisomes | 0.0031 | 0.0002 | 0.0005 | 0.0005 | 0.0019 | 0.001 | 0.0071 | 0.0001 | 0.0058 | 0.0152 | 0.0016 | 0.0008 | 0.0069 | 0.0004 | 0.0006 | 0.0006 | 0.0019 | 0.0007 |
Punctate Nuclear | 0.5363 | 0.4176 | 0.8246 | 0.8606 | 0.9128 | 0.5035 | 0.5654 | 0.5961 | 0.853 | 0.8833 | 0.8618 | 0.5873 | 0.4529 | 0.4555 | 0.7767 | 0.8931 | 0.7808 | 0.4461 |
Vacuole | 0.0012 | 0.0027 | 0.0005 | 0.0002 | 0.0001 | 0.0004 | 0.0017 | 0.0007 | 0.0003 | 0.0002 | 0.0004 | 0.0003 | 0.0017 | 0.0008 | 0.0007 | 0.0001 | 0.0009 | 0.0003 |
Vacuole Periphery | 0.0004 | 0.0005 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0013 | 0.0002 | 0.0002 | 0.0002 | 0.001 | 0.0001 | 0.0012 | 0.0004 | 0.0002 | 0 | 0.0018 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 13.6036 | 13.0616 | 0.6684 | 4.6171 | 12.4117 | 6.0427 | 13.3204 | 7.9915 | 6.177 | 15.0808 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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