Standard name | |
---|---|
Human Ortholog | |
Description | Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN1 has a paralog, ASN2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud | 0 | 0 | 3 | 1 | 0 | 1 | 2 | 4 | 0 | 0 | 1 | 3 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 10 | 52 | 69 | 35 | 11 | 6 | 10 | 6 | 3 | 4 | 5 | 15 | 6 | 12 | 14 | 94 | 80 | 106 | 0 | 10 | 11 | 1 | 0 | 2 |
Cytoplasm | 133 | 131 | 161 | 235 | 284 | 384 | 416 | 349 | 197 | 268 | 263 | 293 | 185 | 270 | 248 | 90 | 81 | 92 | 133 | 243 | 306 | 191 | 170 | 214 |
Endoplasmic Reticulum | 1 | 0 | 1 | 0 | 0 | 1 | 4 | 9 | 5 | 10 | 7 | 14 | 0 | 0 | 0 | 5 | 6 | 2 | 0 | 2 | 6 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 |
Unique Cell Count | 135 | 164 | 213 | 249 | 292 | 391 | 427 | 367 | 204 | 280 | 274 | 321 | 189 | 275 | 251 | 167 | 145 | 171 | 137 | 263 | 335 | 202 | 176 | 228 |
Labelled Cell Count | 144 | 184 | 235 | 273 | 297 | 393 | 434 | 370 | 205 | 282 | 277 | 329 | 194 | 286 | 263 | 189 | 168 | 200 | 137 | 263 | 335 | 202 | 176 | 228 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 493.3 | 565.5 | 536.9 | 471.2 | 406.1 | 340.5 | 299.4 | 273.5 | 246.7 | 254.5 | 225.9 | 249.6 | 438.8 | 437.0 | 441.9 | 646.0 | 661.6 | 653.3 | 476.9 | 484.1 | 489.1 |
Std Deviation (1e-4) | 85.1 | 110.7 | 87.0 | 82.2 | 84.5 | 71.0 | 67.9 | 63.3 | 68.9 | 64.4 | 67.1 | 64.7 | 126.9 | 94.4 | 98.0 | 118.6 | 135.0 | 117.1 | 92.6 | 105.2 | 127.3 |
Intensity Change (Log2) | – | – | – | -0.19 | -0.4 | -0.66 | -0.84 | -0.97 | -1.12 | -1.08 | -1.25 | -1.11 | -0.29 | -0.3 | -0.28 | 0.27 | 0.3 | 0.28 | -0.17 | -0.15 | -0.13 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -4.7 | -8.7 | -11.0 | -10.9 | -10.6 | -8.4 | -9.6 | -9.3 | -8.6 | -7.5 | -8.3 | -7.5 | 4.7 | 4.3 | 5.8 |
Cytoplasm | 5.8 | 7.4 | 8.9 | 8.7 | 7.0 | 6.1 | 6.6 | 6.7 | 5.0 | 6.4 | 7.7 | 7.7 | -4.4 | -3.9 | -4.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 491.8856 | 582.0804 | 518.7791 | 495.8317 | 473.0639 | 522.4252 | 378.5588 | 465.4692 | 378.2098 | 394.0977 | 392.483 | 445.1563 | 441.5915 | 566.7711 | 505.0848 | 382.2734 | 428.7634 | 554.6757 |
Actin | 0.0012 | 0.0002 | 0.0002 | 0.0003 | 0.0003 | 0.0004 | 0.0008 | 0.0001 | 0.0014 | 0.0003 | 0.0002 | 0.0003 | 0.002 | 0.0001 | 0.0051 | 0.0004 | 0.0005 | 0.0002 |
Bud | 0.001 | 0.0002 | 0.0006 | 0.0007 | 0.0014 | 0.0001 | 0.0013 | 0.0003 | 0.0004 | 0.0004 | 0.0002 | 0.0002 | 0.0029 | 0 | 0.0014 | 0.0004 | 0.0008 | 0.0002 |
Bud Neck | 0.0004 | 0.0003 | 0.0004 | 0.0006 | 0.0009 | 0.0017 | 0.0001 | 0.0001 | 0.0003 | 0.0003 | 0.0003 | 0.0008 | 0.0003 | 0.0002 | 0.0007 | 0.0003 | 0.0004 | 0.0006 |
Bud Periphery | 0.0006 | 0 | 0.0002 | 0.0001 | 0.0007 | 0 | 0.0005 | 0.0001 | 0.0006 | 0.0001 | 0 | 0 | 0.0014 | 0 | 0.0006 | 0.0001 | 0.0005 | 0.0001 |
Bud Site | 0.0002 | 0.0001 | 0.0006 | 0.0001 | 0.0003 | 0 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0 | 0 | 0.001 | 0 | 0.0013 | 0.0003 | 0.0005 | 0 |
Cell Periphery | 0.0034 | 0.0029 | 0.0011 | 0.0015 | 0.0017 | 0.0005 | 0.0011 | 0.0002 | 0.0005 | 0.0011 | 0 | 0.0001 | 0.0057 | 0.0019 | 0.0015 | 0.0008 | 0.0057 | 0.0005 |
Cytoplasm | 0.9041 | 0.9775 | 0.9403 | 0.8926 | 0.7869 | 0.9696 | 0.8972 | 0.9773 | 0.8837 | 0.8671 | 0.9828 | 0.9735 | 0.852 | 0.9899 | 0.8767 | 0.7936 | 0.7893 | 0.9598 |
Cytoplasmic Foci | 0.0013 | 0.0001 | 0.0033 | 0.0002 | 0.0102 | 0.0003 | 0.0032 | 0.0011 | 0.0037 | 0.0086 | 0.0004 | 0.0003 | 0.009 | 0 | 0.0211 | 0.0701 | 0.0199 | 0.0002 |
Eisosomes | 0.0003 | 0 | 0.0005 | 0 | 0.0005 | 0 | 0.0007 | 0.0001 | 0.0001 | 0.0003 | 0 | 0 | 0.0009 | 0 | 0.001 | 0.0008 | 0.0015 | 0 |
Endoplasmic Reticulum | 0.0022 | 0.0004 | 0.0002 | 0.0001 | 0.0002 | 0.0004 | 0.0005 | 0.0001 | 0.0059 | 0.0006 | 0.0001 | 0.0007 | 0.0016 | 0.0001 | 0.0006 | 0.0031 | 0.0004 | 0.0001 |
Endosome | 0.002 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0037 | 0.0005 | 0 | 0.0001 | 0.0064 | 0 | 0.0009 | 0.0202 | 0.0004 | 0 |
Golgi | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0004 | 0.0001 | 0 | 0 | 0.0033 | 0 | 0.0004 | 0.0009 | 0.0002 | 0 |
Lipid Particles | 0.0005 | 0.0001 | 0.0004 | 0 | 0.0009 | 0.0001 | 0.0009 | 0.0001 | 0.0003 | 0.003 | 0 | 0 | 0.0018 | 0 | 0.0021 | 0.0026 | 0.0057 | 0.0001 |
Mitochondria | 0.0041 | 0.0003 | 0.0034 | 0.0024 | 0.0348 | 0.0003 | 0.0105 | 0.0043 | 0.0313 | 0.0029 | 0.0002 | 0.0004 | 0.0103 | 0.0001 | 0.0145 | 0.0302 | 0.0261 | 0.001 |
None | 0.0058 | 0.0003 | 0.0074 | 0.0007 | 0.0221 | 0.0004 | 0.0154 | 0.0019 | 0.0043 | 0.0129 | 0.0004 | 0.0005 | 0.0158 | 0.0002 | 0.0229 | 0.0233 | 0.0244 | 0.0007 |
Nuclear Periphery | 0.0187 | 0.0045 | 0.0024 | 0.0113 | 0.0085 | 0.0068 | 0.0174 | 0.0009 | 0.0249 | 0.0099 | 0.0026 | 0.006 | 0.0139 | 0.0023 | 0.0054 | 0.0073 | 0.0092 | 0.0042 |
Nucleolus | 0.0136 | 0.0002 | 0.0205 | 0.0054 | 0.0377 | 0.0003 | 0.011 | 0.0021 | 0.0051 | 0.0156 | 0 | 0.0001 | 0.0235 | 0.0001 | 0.0252 | 0.0174 | 0.0377 | 0.0091 |
Nucleus | 0.0323 | 0.0108 | 0.0131 | 0.0818 | 0.0764 | 0.0166 | 0.0338 | 0.01 | 0.0263 | 0.0613 | 0.0119 | 0.0163 | 0.036 | 0.0048 | 0.0117 | 0.0198 | 0.0261 | 0.0216 |
Peroxisomes | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0009 | 0 | 0.0004 | 0.0001 | 0.0002 | 0.0003 | 0 | 0 | 0.0006 | 0 | 0.0009 | 0.0012 | 0.0016 | 0 |
Punctate Nuclear | 0.0005 | 0 | 0.0003 | 0.0001 | 0.0006 | 0.0002 | 0.0004 | 0.0001 | 0.0007 | 0.0019 | 0.0002 | 0.0002 | 0.002 | 0 | 0.0018 | 0.0013 | 0.0009 | 0.0002 |
Vacuole | 0.005 | 0.0017 | 0.0033 | 0.0011 | 0.0073 | 0.0016 | 0.0023 | 0.0006 | 0.0026 | 0.0057 | 0.0003 | 0.0004 | 0.0068 | 0.0004 | 0.0022 | 0.0039 | 0.0272 | 0.0009 |
Vacuole Periphery | 0.0024 | 0.0002 | 0.0019 | 0.0009 | 0.0076 | 0.0005 | 0.0018 | 0.0004 | 0.0032 | 0.0068 | 0.0002 | 0.0002 | 0.0029 | 0 | 0.0019 | 0.0019 | 0.0209 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 630.0125 | 686.8483 | 688.9289 | 693.0242 | 753.1727 | 542.0946 | 647.7674 | 581.8882 | 635.2805 | 692.4835 |
Translational Efficiency | 0.8628 | 0.9359 | 0.9008 | 1.0432 | 0.9909 | 0.9621 | 1.0283 | 1.023 | 1.0197 | 1.0597 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|