Standard name
Human Ortholog
Description Protein of unknown function containing WW and FF domains; overexpression causes accumulation of cells in G1 phase

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0.06 0.07 0.07 0 0.07 0 0 0.08 0.15 0.15 0.12 0.08 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.13 0 0.14 0.29 0.19 0.49 0.53 0.49 0.58 0.64 0.57 0.55 0 0 0 0.08 0.05 0 0 0 0 0 0 0
Nucleus 0.93 0.92 0.92 0.9 0.81 0.73 0.69 0.69 0.7 0.65 0.72 0.76 0.9 0.8 0.75 0.77 0.83 0.82 0.87 0.89 0.88 0.72 0.63 0.43
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.05 0 0 0 0 0 0 0 0 0 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0.07 0.1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0 0 0.08 0.12 0.1 0.15 0.05 0.05 0.06 0.13 0.19 0.3
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0
Bud 1 0 0 0 1 0 3 5 5 5 8 3 0 1 0 0 0 0 2 2 2 1 2 3
Bud Neck 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 2 0 0 3 1 7 9 4 8 12 13 15 0 0 0 0 0 1
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 4 14 4 5 12 20 30 36 14 23 5 11 22 73 61 38 15 8 0 1 0 1 1 3
Endoplasmic Reticulum 0 1 0 0 0 0 0 1 0 0 0 0 1 1 1 8 6 4 0 1 0 2 0 3
Endosome 0 0 0 0 0 0 1 0 0 0 0 0 0 2 4 4 0 0 2 3 0 4 5 16
Golgi 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 2 0 1 0 4 1 2 1 2
Mitochondria 17 6 23 56 43 177 243 245 216 219 194 150 5 10 9 25 10 3 0 3 0 3 10 9
Nucleus 124 348 152 172 185 262 314 347 261 223 245 208 235 400 310 241 151 103 124 336 144 151 230 168
Nuclear Periphery 3 0 0 5 1 2 6 12 6 6 7 2 2 4 3 1 1 0 0 0 0 0 2 5
Nucleolus 7 2 1 5 2 9 10 12 14 13 25 15 0 11 14 0 0 1 1 0 0 7 25 38
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 1 2 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 1 0 1 0 1 1 0 0 3 2 0 2 1 0 1 0 5 6 7
Vac/Vac Membrane 3 17 2 0 11 22 14 12 10 5 7 10 1 22 31 37 18 19 7 17 9 27 68 116
Unique Cell Count 134 378 165 191 228 361 455 501 371 342 342 275 261 502 413 315 182 126 142 377 165 212 363 390
Labelled Cell Count 161 388 182 247 257 500 630 676 534 508 505 414 267 529 436 356 203 141 142 377 165 212 363 390


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 6.0 4.9 4.5 4.9 4.2 3.8 4.1 4.2 3.9 4.0 4.1 5.6 5.4 5.6 5.9 6.7 6.8 5.5 5.4 6.3
Std Deviation (1e-4) 0.8 1.0 0.8 1.0 1.5 1.1 1.1 1.3 1.0 1.2 1.0 1.2 1.0 1.2 1.3 1.6 1.3 1.6 1.2 1.1 1.9
Intensity Change (Log2) -0.1 0.01 -0.21 -0.36 -0.25 -0.22 -0.33 -0.27 -0.25 0.19 0.16 0.2 0.28 0.47 0.48 0.18 0.15 0.38


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 1.4 1.6 2.0 2.2 0.8 2.0 0 0.9 2.5 4.2 4.2 3.5 2.4 1.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 1.3 0 0 0 0 0 0 0 -4.9 -6.1 -5.6 -2.1 -2.7 -3.4
Nucleus -0.7 -3.1 -5.1 -5.9 -5.9 -5.5 -6.5 -5.2 -4.3 -0.7 -3.7 -4.6 -4.2 -2.6 -2.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 2.0 2.5 0 0 0 0 0 0 0 1.9 2.9 4.0 3.5 4.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.2518 1.0834 0.5544 0.6581 0.1172 0.6475 3.1107 3.1218 2.9364 2.8837 2.4152 3.0947 4.8944 3.5858 3.6924 3.3636 3.7424 3.9149
Actin 0.0078 0 0.0001 0 0.0003 0.0001 0.0273 0.0002 0.0172 0.0389 0.0122 0.0015 0.0544 0.0002 0.0155 0.0002 0.0046 0.0107
Bud 0.0001 0 0.0001 0 0 0 0.0009 0 0.0002 0.0004 0.0002 0.0002 0.0006 0.0027 0.001 0 0.0007 0.0012
Bud Neck 0.0005 0.0001 0.0029 0.0003 0.0006 0.0011 0.0074 0.0002 0.0008 0.0006 0.0009 0.0012 0.0012 0.0004 0.0014 0.0004 0.0015 0.0024
Bud Periphery 0.0003 0 0.0004 0 0 0 0.0036 0.0001 0.0005 0.0005 0.0003 0.0009 0.0015 0.0008 0.0018 0 0.0047 0.0024
Bud Site 0.0006 0.0001 0.0003 0.0001 0 0 0.0042 0.0002 0.001 0.0018 0.0014 0.0006 0.0031 0.0012 0.0102 0.0001 0.001 0.0006
Cell Periphery 0.0002 0 0.0002 0 0 0 0.0006 0 0.0001 0.0002 0.0001 0.0002 0.0004 0.0001 0.0002 0 0.0005 0.0002
Cytoplasm 0.0046 0.0063 0.0081 0.0042 0.0011 0.001 0.0085 0.0035 0.0034 0.0025 0.0015 0.0015 0.0159 0.0121 0.005 0.0031 0.0079 0.0083
Cytoplasmic Foci 0.0014 0.0002 0.0003 0.0001 0.0001 0.0001 0.0142 0 0.0098 0.0019 0.0087 0.0047 0.0199 0.0105 0.004 0.0001 0.0003 0.0005
Eisosomes 0.0002 0 0 0 0.0003 0 0.0008 0 0.0001 0.0002 0.0003 0.0001 0.0005 0 0.0001 0 0.0003 0.0001
Endoplasmic Reticulum 0.0006 0.0012 0.0003 0.0001 0.0001 0.0001 0.0047 0.0005 0.0005 0.0003 0.0003 0.0004 0.0207 0.0004 0.0017 0.0004 0.0035 0.0013
Endosome 0.0057 0.0014 0.0007 0.0003 0.0002 0.0002 0.0204 0.0004 0.0049 0.0031 0.0185 0.0107 0.0279 0.0006 0.0087 0.0004 0.0047 0.0025
Golgi 0.0006 0 0 0 0 0 0.0059 0 0.0053 0.0082 0.0258 0.0054 0.0083 0.0001 0.0023 0 0.0015 0.001
Lipid Particles 0.0017 0.0001 0.0002 0 0.0001 0 0.0226 0 0.0017 0.0024 0.0085 0.0024 0.0268 0.0002 0.0066 0 0.0024 0.0006
Mitochondria 0.0013 0.0001 0.0002 0.0001 0.0008 0.0001 0.0071 0.0006 0.0093 0.0576 0.023 0.0198 0.0157 0.0024 0.0012 0.0003 0.0429 0.0063
None 0.0208 0.0015 0.0318 0.0035 0.045 0.011 0.0207 0.0028 0.0014 0.0006 0.0355 0.0058 0.0324 0.0038 0.0013 0.0016 0.0035 0.0125
Nuclear Periphery 0.0162 0.0195 0.0098 0.0041 0.0096 0.0106 0.0335 0.025 0.0103 0.0032 0.0057 0.0018 0.0458 0.0075 0.0035 0.0157 0.0482 0.0084
Nucleolus 0.0185 0.0095 0.0143 0.0273 0.0364 0.0318 0.0104 0.0211 0.0209 0.0182 0.0215 0.025 0.0112 0.0205 0.0054 0.0078 0.0231 0.0262
Nucleus 0.8859 0.9365 0.9032 0.9444 0.8475 0.9171 0.7672 0.9392 0.8935 0.8546 0.8258 0.9059 0.6579 0.9086 0.908 0.9315 0.8345 0.8963
Peroxisomes 0.0006 0 0.0001 0 0.0001 0 0.0129 0 0.0043 0.0009 0.0062 0.0032 0.0221 0.0002 0.0013 0 0.0006 0.0008
Punctate Nuclear 0.0296 0.0221 0.0253 0.0149 0.0574 0.0265 0.0152 0.0052 0.0058 0.0032 0.0021 0.006 0.0235 0.0264 0.0191 0.0376 0.0059 0.015
Vacuole 0.0022 0.001 0.0015 0.0002 0.0002 0.0001 0.0087 0.0005 0.0056 0.0005 0.0007 0.0023 0.0042 0.0011 0.0012 0.0005 0.0043 0.0018
Vacuole Periphery 0.0005 0.0003 0.0002 0.0001 0.0002 0.0001 0.0034 0.0005 0.0031 0.0005 0.0007 0.0006 0.0058 0.0003 0.0005 0.0002 0.0036 0.0012

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3.9464 7.7612 6.8063 6.4173 8.6983 1.5777 6.9556 6.5001 9.1737 6.9237
Translational Efficiency

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Protein of unknown function containing WW and FF domains; overexpression causes accumulation of cells in G1 phase
Localization
Cell Percentages nucleus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Urn1

Urn1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Urn1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available