Standard name | |
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Human Ortholog | |
Description | Protein of unknown function containing WW and FF domains; overexpression causes accumulation of cells in G1 phase |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 |
Bud | 1 | 0 | 0 | 0 | 1 | 0 | 3 | 5 | 5 | 5 | 8 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 2 | 2 | 1 | 2 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 2 | 0 | 0 | 3 | 1 | 7 | 9 | 4 | 8 | 12 | 13 | 15 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 14 | 4 | 5 | 12 | 20 | 30 | 36 | 14 | 23 | 5 | 11 | 22 | 73 | 61 | 38 | 15 | 8 | 0 | 1 | 0 | 1 | 1 | 3 |
Endoplasmic Reticulum | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 8 | 6 | 4 | 0 | 1 | 0 | 2 | 0 | 3 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 4 | 4 | 0 | 0 | 2 | 3 | 0 | 4 | 5 | 16 |
Golgi | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 4 | 1 | 2 | 1 | 2 |
Mitochondria | 17 | 6 | 23 | 56 | 43 | 177 | 243 | 245 | 216 | 219 | 194 | 150 | 5 | 10 | 9 | 25 | 10 | 3 | 0 | 3 | 0 | 3 | 10 | 9 |
Nucleus | 124 | 348 | 152 | 172 | 185 | 262 | 314 | 347 | 261 | 223 | 245 | 208 | 235 | 400 | 310 | 241 | 151 | 103 | 124 | 336 | 144 | 151 | 230 | 168 |
Nuclear Periphery | 3 | 0 | 0 | 5 | 1 | 2 | 6 | 12 | 6 | 6 | 7 | 2 | 2 | 4 | 3 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 5 |
Nucleolus | 7 | 2 | 1 | 5 | 2 | 9 | 10 | 12 | 14 | 13 | 25 | 15 | 0 | 11 | 14 | 0 | 0 | 1 | 1 | 0 | 0 | 7 | 25 | 38 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 3 | 2 | 0 | 2 | 1 | 0 | 1 | 0 | 5 | 6 | 7 |
Vac/Vac Membrane | 3 | 17 | 2 | 0 | 11 | 22 | 14 | 12 | 10 | 5 | 7 | 10 | 1 | 22 | 31 | 37 | 18 | 19 | 7 | 17 | 9 | 27 | 68 | 116 |
Unique Cell Count | 134 | 378 | 165 | 191 | 228 | 361 | 455 | 501 | 371 | 342 | 342 | 275 | 261 | 502 | 413 | 315 | 182 | 126 | 142 | 377 | 165 | 212 | 363 | 390 |
Labelled Cell Count | 161 | 388 | 182 | 247 | 257 | 500 | 630 | 676 | 534 | 508 | 505 | 414 | 267 | 529 | 436 | 356 | 203 | 141 | 142 | 377 | 165 | 212 | 363 | 390 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.8 | 6.0 | 4.9 | 4.5 | 4.9 | 4.2 | 3.8 | 4.1 | 4.2 | 3.9 | 4.0 | 4.1 | 5.6 | 5.4 | 5.6 | 5.9 | 6.7 | 6.8 | 5.5 | 5.4 | 6.3 |
Std Deviation (1e-4) | 0.8 | 1.0 | 0.8 | 1.0 | 1.5 | 1.1 | 1.1 | 1.3 | 1.0 | 1.2 | 1.0 | 1.2 | 1.0 | 1.2 | 1.3 | 1.6 | 1.3 | 1.6 | 1.2 | 1.1 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.1 | 0.01 | -0.21 | -0.36 | -0.25 | -0.22 | -0.33 | -0.27 | -0.25 | 0.19 | 0.16 | 0.2 | 0.28 | 0.47 | 0.48 | 0.18 | 0.15 | 0.38 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 1.4 | 1.6 | 2.0 | 2.2 | 0.8 | 2.0 | 0 | 0.9 | 2.5 | 4.2 | 4.2 | 3.5 | 2.4 | 1.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 1.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -4.9 | -6.1 | -5.6 | -2.1 | -2.7 | -3.4 |
Nucleus | -0.7 | -3.1 | -5.1 | -5.9 | -5.9 | -5.5 | -6.5 | -5.2 | -4.3 | -0.7 | -3.7 | -4.6 | -4.2 | -2.6 | -2.7 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 2.0 | 2.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.9 | 2.9 | 4.0 | 3.5 | 4.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.2518 | 1.0834 | 0.5544 | 0.6581 | 0.1172 | 0.6475 | 3.1107 | 3.1218 | 2.9364 | 2.8837 | 2.4152 | 3.0947 | 4.8944 | 3.5858 | 3.6924 | 3.3636 | 3.7424 | 3.9149 |
Actin | 0.0078 | 0 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0273 | 0.0002 | 0.0172 | 0.0389 | 0.0122 | 0.0015 | 0.0544 | 0.0002 | 0.0155 | 0.0002 | 0.0046 | 0.0107 |
Bud | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0009 | 0 | 0.0002 | 0.0004 | 0.0002 | 0.0002 | 0.0006 | 0.0027 | 0.001 | 0 | 0.0007 | 0.0012 |
Bud Neck | 0.0005 | 0.0001 | 0.0029 | 0.0003 | 0.0006 | 0.0011 | 0.0074 | 0.0002 | 0.0008 | 0.0006 | 0.0009 | 0.0012 | 0.0012 | 0.0004 | 0.0014 | 0.0004 | 0.0015 | 0.0024 |
Bud Periphery | 0.0003 | 0 | 0.0004 | 0 | 0 | 0 | 0.0036 | 0.0001 | 0.0005 | 0.0005 | 0.0003 | 0.0009 | 0.0015 | 0.0008 | 0.0018 | 0 | 0.0047 | 0.0024 |
Bud Site | 0.0006 | 0.0001 | 0.0003 | 0.0001 | 0 | 0 | 0.0042 | 0.0002 | 0.001 | 0.0018 | 0.0014 | 0.0006 | 0.0031 | 0.0012 | 0.0102 | 0.0001 | 0.001 | 0.0006 |
Cell Periphery | 0.0002 | 0 | 0.0002 | 0 | 0 | 0 | 0.0006 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0004 | 0.0001 | 0.0002 | 0 | 0.0005 | 0.0002 |
Cytoplasm | 0.0046 | 0.0063 | 0.0081 | 0.0042 | 0.0011 | 0.001 | 0.0085 | 0.0035 | 0.0034 | 0.0025 | 0.0015 | 0.0015 | 0.0159 | 0.0121 | 0.005 | 0.0031 | 0.0079 | 0.0083 |
Cytoplasmic Foci | 0.0014 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0142 | 0 | 0.0098 | 0.0019 | 0.0087 | 0.0047 | 0.0199 | 0.0105 | 0.004 | 0.0001 | 0.0003 | 0.0005 |
Eisosomes | 0.0002 | 0 | 0 | 0 | 0.0003 | 0 | 0.0008 | 0 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0005 | 0 | 0.0001 | 0 | 0.0003 | 0.0001 |
Endoplasmic Reticulum | 0.0006 | 0.0012 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0047 | 0.0005 | 0.0005 | 0.0003 | 0.0003 | 0.0004 | 0.0207 | 0.0004 | 0.0017 | 0.0004 | 0.0035 | 0.0013 |
Endosome | 0.0057 | 0.0014 | 0.0007 | 0.0003 | 0.0002 | 0.0002 | 0.0204 | 0.0004 | 0.0049 | 0.0031 | 0.0185 | 0.0107 | 0.0279 | 0.0006 | 0.0087 | 0.0004 | 0.0047 | 0.0025 |
Golgi | 0.0006 | 0 | 0 | 0 | 0 | 0 | 0.0059 | 0 | 0.0053 | 0.0082 | 0.0258 | 0.0054 | 0.0083 | 0.0001 | 0.0023 | 0 | 0.0015 | 0.001 |
Lipid Particles | 0.0017 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0226 | 0 | 0.0017 | 0.0024 | 0.0085 | 0.0024 | 0.0268 | 0.0002 | 0.0066 | 0 | 0.0024 | 0.0006 |
Mitochondria | 0.0013 | 0.0001 | 0.0002 | 0.0001 | 0.0008 | 0.0001 | 0.0071 | 0.0006 | 0.0093 | 0.0576 | 0.023 | 0.0198 | 0.0157 | 0.0024 | 0.0012 | 0.0003 | 0.0429 | 0.0063 |
None | 0.0208 | 0.0015 | 0.0318 | 0.0035 | 0.045 | 0.011 | 0.0207 | 0.0028 | 0.0014 | 0.0006 | 0.0355 | 0.0058 | 0.0324 | 0.0038 | 0.0013 | 0.0016 | 0.0035 | 0.0125 |
Nuclear Periphery | 0.0162 | 0.0195 | 0.0098 | 0.0041 | 0.0096 | 0.0106 | 0.0335 | 0.025 | 0.0103 | 0.0032 | 0.0057 | 0.0018 | 0.0458 | 0.0075 | 0.0035 | 0.0157 | 0.0482 | 0.0084 |
Nucleolus | 0.0185 | 0.0095 | 0.0143 | 0.0273 | 0.0364 | 0.0318 | 0.0104 | 0.0211 | 0.0209 | 0.0182 | 0.0215 | 0.025 | 0.0112 | 0.0205 | 0.0054 | 0.0078 | 0.0231 | 0.0262 |
Nucleus | 0.8859 | 0.9365 | 0.9032 | 0.9444 | 0.8475 | 0.9171 | 0.7672 | 0.9392 | 0.8935 | 0.8546 | 0.8258 | 0.9059 | 0.6579 | 0.9086 | 0.908 | 0.9315 | 0.8345 | 0.8963 |
Peroxisomes | 0.0006 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0129 | 0 | 0.0043 | 0.0009 | 0.0062 | 0.0032 | 0.0221 | 0.0002 | 0.0013 | 0 | 0.0006 | 0.0008 |
Punctate Nuclear | 0.0296 | 0.0221 | 0.0253 | 0.0149 | 0.0574 | 0.0265 | 0.0152 | 0.0052 | 0.0058 | 0.0032 | 0.0021 | 0.006 | 0.0235 | 0.0264 | 0.0191 | 0.0376 | 0.0059 | 0.015 |
Vacuole | 0.0022 | 0.001 | 0.0015 | 0.0002 | 0.0002 | 0.0001 | 0.0087 | 0.0005 | 0.0056 | 0.0005 | 0.0007 | 0.0023 | 0.0042 | 0.0011 | 0.0012 | 0.0005 | 0.0043 | 0.0018 |
Vacuole Periphery | 0.0005 | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0034 | 0.0005 | 0.0031 | 0.0005 | 0.0007 | 0.0006 | 0.0058 | 0.0003 | 0.0005 | 0.0002 | 0.0036 | 0.0012 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 3.9464 | 7.7612 | 6.8063 | 6.4173 | 8.6983 | 1.5777 | 6.9556 | 6.5001 | 9.1737 | 6.9237 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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