Standard name
Human Ortholog
Description Negative regulator of actin nucleation-promoting factor activity; interacts with Las17p, a homolog of human Wiskott-Aldrich Syndrome protein (WASP), via an N-terminal SH3 domain, and along with LSB1 cooperatively inhibits the nucleation of actin filaments; short-lived protein whose levels increase in response to thermal stress; induces the formation of the [PIN+] and [RNQ+] prions when overproduced; PIN3 has a paralog, LSB1, that arose from the whole genome duplication
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.95 0.98 0.97 0.9 0.83 0.83 0.78 0.67 0.75 0.7 0.72 0.68 0.98 0.97 0.97 0.84 0.9 0.92 0.9 0.91 0.94 0.94 0.91 0.86
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.11 0 0.05 0 0.09 0.1 0.13 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.08 0.25 0.07 0.27 0.31 0.21 0.21 0.17 0.19 0 0 0.05 0.18 0.1 0.09 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 1 0 1 0 0 0 0 0 0 0 0 1 3 0 0 2 2
Bud 0 0 0 0 0 3 0 1 1 1 2 0 0 0 0 0 1 0 0 0 3 0 2 8
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 5 2 3 1 3 8 2 7 8 7 1 1 2 4 4 4 0 0 0 0 0 0
Cytoplasm 161 330 188 185 269 138 209 244 174 203 149 140 315 407 422 178 179 175 161 310 182 308 341 378
Endoplasmic Reticulum 1 6 2 1 3 1 2 1 1 4 4 7 6 0 1 5 2 5 1 2 0 1 6 8
Endosome 0 0 1 0 0 0 0 1 0 0 1 1 0 0 0 0 0 0 3 3 1 0 3 14
Golgi 1 1 0 0 0 0 0 0 1 0 0 0 0 0 0 1 2 1 2 1 0 1 2 2
Mitochondria 3 1 0 3 1 18 6 17 10 27 21 26 1 0 0 7 3 2 1 3 0 0 1 0
Nucleus 0 2 1 1 1 4 4 6 3 13 6 9 4 1 0 4 5 3 0 3 2 2 2 4
Nuclear Periphery 0 1 0 0 0 0 0 0 0 2 1 2 0 0 0 0 0 0 1 0 0 0 0 4
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 3 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 2 4
Vac/Vac Membrane 4 1 5 17 81 12 73 112 50 61 35 40 3 18 20 38 19 17 2 4 0 4 3 4
Unique Cell Count 169 338 194 205 323 166 267 366 233 290 208 206 322 418 437 211 200 190 179 340 194 327 373 441
Labelled Cell Count 170 342 202 209 358 178 297 391 242 319 227 233 330 427 445 241 215 207 179 340 194 327 373 441


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.7 9.8 9.9 9.5 11.1 7.1 9.9 9.7 9.0 9.0 9.0 8.6 9.3 10.6 10.9 16.8 16.2 15.2 8.8 10.1 11.1
Std Deviation (1e-4) 1.2 1.8 1.7 1.5 1.7 1.7 2.2 1.9 2.3 2.1 2.4 2.0 1.9 2.0 1.9 6.1 5.2 5.8 1.8 2.0 2.3
Intensity Change (Log2) – – – -0.07 0.16 -0.49 -0.01 -0.04 -0.15 -0.15 -0.15 -0.2 -0.09 0.1 0.14 0.75 0.71 0.61 -0.17 0.02 0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0.7 0.5 0 0 0 0 0 0
Cytoplasm -2.7 -4.7 -4.5 -5.7 -8.1 -6.4 -7.4 -6.9 -7.5 0.6 0.3 -0.2 -4.3 -2.9 -2.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.7 0 3.0 0 4.4 4.5 5.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 2.6 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 2.5 6.7 2.1 7.0 7.8 5.8 5.8 4.8 5.3 0 1.0 1.2 5.0 2.9 2.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.8839 5.3711 4.1951 3.4719 3.591 4.1487 5.5378 7.1806 6.1087 5.1042 5.767 6.1453 7.9033 9.8486 8.5967 8.8534 8.211 8.5948
Actin 0.0336 0.0024 0.0218 0.0323 0.0302 0.0059 0.0324 0.0035 0.0285 0.0849 0.046 0.0057 0.0594 0.0012 0.0276 0.0027 0.0256 0.0034
Bud 0.0009 0.0131 0.0036 0.0012 0.002 0.001 0.0011 0.0001 0.0021 0.0015 0.0003 0.0003 0.0008 0.0001 0.0007 0.0005 0.0015 0.0001
Bud Neck 0.0051 0.0006 0.0023 0.0025 0.0048 0.0035 0.0025 0.0003 0.0022 0.002 0.0011 0.0019 0.008 0.0003 0.0049 0.0009 0.0011 0.0018
Bud Periphery 0.0008 0.0028 0.0015 0.0016 0.0022 0.0005 0.0021 0 0.0022 0.002 0.0004 0.0002 0.0014 0 0.0012 0.0002 0.0013 0.0001
Bud Site 0.0035 0.0119 0.0084 0.0024 0.0048 0.0002 0.0027 0.0017 0.0164 0.0013 0.0009 0.0002 0.0079 0.0005 0.0059 0.0008 0.0004 0.0001
Cell Periphery 0.0002 0.0003 0.0005 0.0003 0.0003 0.0001 0.0002 0.0001 0.0008 0.0002 0.0001 0 0.0012 0.0001 0.0012 0.0001 0.0001 0.0001
Cytoplasm 0.7266 0.911 0.8656 0.808 0.7237 0.9002 0.8279 0.9776 0.8553 0.8541 0.8743 0.9126 0.8438 0.9823 0.9048 0.9556 0.895 0.9443
Cytoplasmic Foci 0.0425 0.0138 0.0203 0.016 0.0285 0.0231 0.0271 0.0036 0.01 0.0027 0.0094 0.0125 0.005 0.0015 0.0042 0.0078 0.0034 0.0026
Eisosomes 0.0003 0 0.0005 0.0003 0.0013 0 0.0001 0.0001 0.0003 0.0003 0.0002 0 0.0007 0 0.0009 0 0.0001 0.0002
Endoplasmic Reticulum 0.008 0.0015 0.005 0.0048 0.0061 0.0033 0.0025 0.0011 0.003 0.0017 0.0062 0.0027 0.007 0.0012 0.0038 0.0025 0.0065 0.0035
Endosome 0.0472 0.0015 0.0103 0.019 0.0471 0.0104 0.0223 0.0006 0.0163 0.007 0.0063 0.0105 0.008 0.0002 0.003 0.0026 0.0117 0.003
Golgi 0.015 0.0003 0.0036 0.0178 0.0178 0.0018 0.0122 0.0003 0.0139 0.0157 0.004 0.0058 0.0031 0.0001 0.0018 0.0004 0.0021 0.0008
Lipid Particles 0.0164 0.0001 0.0025 0.0058 0.0129 0.0016 0.009 0.0007 0.0057 0.0019 0.0096 0.0041 0.0013 0 0.0021 0.0004 0.0016 0.0005
Mitochondria 0.0056 0.0006 0.0052 0.0212 0.0113 0.0035 0.0104 0.0002 0.0135 0.0075 0.0011 0.0048 0.0015 0.0001 0.0012 0.0002 0.0031 0.0009
None 0.0248 0.015 0.0169 0.032 0.0347 0.0115 0.0167 0.0052 0.0074 0.0035 0.0057 0.0127 0.0245 0.0059 0.0263 0.0042 0.0078 0.0152
Nuclear Periphery 0.0119 0.0016 0.0032 0.0076 0.0128 0.0067 0.0029 0.0006 0.0016 0.0011 0.0025 0.0016 0.0083 0.0007 0.0018 0.0028 0.021 0.0054
Nucleolus 0.0005 0.0003 0.0005 0.0004 0.003 0.0001 0.0003 0 0.0024 0.0001 0.0002 0 0.0005 0 0.0002 0.0001 0.0005 0.0007
Nucleus 0.0214 0.0172 0.0163 0.0169 0.0244 0.0149 0.015 0.0032 0.0103 0.0099 0.0053 0.009 0.0083 0.0052 0.0057 0.0104 0.0086 0.0146
Peroxisomes 0.0223 0.0032 0.0057 0.002 0.0073 0.0041 0.007 0.0004 0.0031 0.0006 0.0192 0.0137 0.0008 0 0.0005 0.0002 0.0005 0.0001
Punctate Nuclear 0.0096 0.002 0.0042 0.0057 0.0198 0.0058 0.0025 0.0003 0.0019 0.0005 0.006 0.001 0.0073 0.0003 0.001 0.0071 0.0063 0.002
Vacuole 0.0023 0.0007 0.0017 0.0015 0.0034 0.0014 0.0018 0.0003 0.0021 0.0008 0.0009 0.0003 0.0008 0.0002 0.0009 0.0005 0.0009 0.0004
Vacuole Periphery 0.0014 0.0001 0.0004 0.0009 0.0015 0.0004 0.001 0 0.0009 0.0006 0.0002 0.0003 0.0005 0 0.0002 0.0001 0.0008 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 40.8668 24.355 33.8858 46.7354 26.4928 44.2479 50.7896 59.2102 44.463 47.7991
Translational Efficiency 2.184 3.1075 1.8488 1.0241 2.5955 2.958 1.9678 1.8235 2.3387 1.8345

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Negative regulator of actin nucleation-promoting factor activity; interacts with Las17p, a homolog of human Wiskott-Aldrich Syndrome protein (WASP), via an N-terminal SH3 domain, and along with LSB1 cooperatively inhibits the nucleation of actin filaments; short-lived protein whose levels increase in response to thermal stress; induces the formation of the [PIN+] and [RNQ+] prions when overproduced; PIN3 has a paralog, LSB1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Pin3

Pin3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pin3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available