Standard name
Human Ortholog
Description Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; specific for spermine; localizes to the plasma membrane; TPO3 has a paralog, TPO2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.16 0.3 0.26 0.27 0.25 0.21 0.27 0.24 0.25 0.32 0.23 0.28 0.17 0.09 0.08 0.09 0.06 0.09 0 0 0 0 0 0
Bud 0.05 0 0 0 0 0 0 0.08 0.11 0 0.07 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.73 0.79 0.71 0.61 0.56 0.4 0.48 0.42 0.51 0.5 0.52 0.49 0.78 0.77 0.8 0.76 0.72 0.7 0.62 0.73 0.71 0.73 0.8 0.83
Cytoplasm 0.15 0.11 0.11 0.27 0.22 0.33 0.27 0.28 0.32 0.26 0.24 0.26 0.17 0.15 0.17 0.2 0.23 0.29 0 0 0 0 0 0
Endoplasmic Reticulum 0.19 0.16 0.23 0.19 0.32 0.26 0.2 0.15 0.06 0.1 0.07 0.09 0.14 0.2 0.14 0 0 0 0.26 0.17 0.21 0.19 0.1 0.06
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.05 0.15 0.08 0.2 0.21 0.28 0.33 0.35 0.42 0.46 0 0 0 0.1 0.09 0.08 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 51 119 65 52 62 67 96 114 79 117 80 107 119 74 68 29 17 22 5 11 9 9 13 8
Bud 15 13 10 8 7 14 13 38 34 15 24 21 14 8 8 4 6 2 4 3 0 1 3 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 4 2 1 4 6
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 229 316 179 120 141 125 168 198 165 183 177 187 537 601 651 245 194 180 220 349 204 251 351 344
Cytoplasm 46 44 28 53 55 104 95 133 102 93 81 98 119 120 142 65 62 74 14 13 4 6 11 10
Endoplasmic Reticulum 61 64 58 38 80 80 69 72 20 38 23 35 99 156 111 5 8 4 92 81 60 65 42 26
Endosome 0 0 0 0 0 1 0 0 0 0 0 0 2 0 0 0 0 1 0 0 0 0 1 0
Golgi 0 1 1 0 0 0 0 1 0 0 0 0 2 0 0 3 0 0 2 0 0 0 1 1
Mitochondria 8 12 13 29 20 64 75 129 106 126 144 173 2 0 0 33 23 21 2 5 4 3 1 6
Nucleus 0 0 1 0 2 0 1 1 0 1 1 3 1 0 2 1 1 1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 4 0 4 3 6 0 0 0 1 1 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 1 0 1 1 1 1 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 2 0 0 0 0 1 0 0 0 1 3 1 4 1 0 1 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 1 0 1 1 1 3 6 0 3 4 0 4 2 1 1 2 2
Unique Cell Count 313 401 252 196 251 313 352 468 321 363 342 380 690 784 813 321 270 258 354 477 290 345 439 415
Labelled Cell Count 410 570 357 301 368 456 518 693 506 579 535 633 901 966 986 390 316 306 354 477 290 345 439 415


Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.1 16.5 11.8 10.8 11.1 10.0 10.4 10.6 10.1 9.5 10.0 9.8 13.6 13.7 13.9 11.5 13.1 15.0 13.7 12.9 12.4
Std Deviation (1e-4) 3.3 4.4 3.0 2.8 2.5 2.5 2.3 2.4 2.1 1.9 2.2 2.1 3.1 2.7 2.7 2.3 3.2 3.3 3.2 2.5 2.2
Intensity Change (Log2) – – – -0.13 -0.08 -0.24 -0.18 -0.15 -0.22 -0.32 -0.24 -0.26 0.21 0.21 0.24 -0.03 0.15 0.34 0.21 0.13 0.07


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.2 -0.3 -1.2 0.4 -0.4 -0.3 1.7 -0.7 0.7 -2.9 -6.6 -7.3 -5.4 -6.1 -5.2
Bud 0.1 -0.7 0.3 -0.2 2.1 3.0 0.1 1.6 0.9 -1.7 0 0 -2.1 -1.2 -2.4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -2.2 -3.5 -7.4 -5.7 -7.4 -4.8 -5.1 -4.7 -5.4 2.2 1.8 3.0 1.4 0.2 -0.3
Cytoplasm 4.3 3.3 6.2 4.8 5.3 5.9 4.5 3.9 4.5 2.3 1.7 2.4 2.9 3.6 5.0
Endoplasmic Reticulum -0.9 2.2 0.7 -1.0 -2.5 -5.8 -4.2 -5.7 -4.8 -3.2 -1.1 -3.6 -8.2 -6.9 -7.4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.5 1.3 5.3 5.5 7.2 8.2 8.6 10.1 10.9 0 0 0 2.2 1.5 1.3
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 12.025 15.5008 13.325 13.305 12.8596 15.3362 13.2156 15.9845 13.7492 13.8438 15.3038 15.593 16.9913 17.074 16.5754 16.4595 17.409 18.9239
Actin 0.0259 0.0013 0.029 0.0513 0.0004 0.0007 0.0521 0.0073 0.0002 0 0.0023 0.0001 0.0255 0.0002 0.0001 0.0001 0 0.0001
Bud 0.0024 0.0004 0.0018 0.001 0.0002 0.0005 0.001 0.0003 0.0001 0.0001 0.0002 0.0001 0.0003 0.0001 0.0001 0.0001 0 0.0001
Bud Neck 0.0014 0.0005 0.0008 0.0013 0.0001 0.0006 0.0038 0.0003 0.0005 0.0003 0.0004 0.0011 0.0003 0.0005 0.0003 0.0016 0.0003 0.0004
Bud Periphery 0.0118 0.0025 0.0092 0.0046 0.0049 0.0107 0.004 0.0012 0.0019 0.0026 0.0012 0.0033 0.0013 0.001 0.0014 0.0037 0.0013 0.0037
Bud Site 0.008 0.018 0.0063 0.0039 0.0001 0.0002 0.0126 0.018 0.0137 0.0003 0.0005 0.0001 0.0017 0.0384 0.0016 0.0003 0.0001 0.0001
Cell Periphery 0.622 0.8303 0.8179 0.6223 0.7064 0.7526 0.6365 0.8278 0.8901 0.7798 0.6397 0.7898 0.7657 0.8132 0.8727 0.5343 0.7052 0.8364
Cytoplasm 0.042 0.0715 0.0487 0.0679 0.014 0.0249 0.0245 0.0392 0.0241 0.0145 0.0219 0.0199 0.0119 0.0399 0.0276 0.0203 0.0292 0.0123
Cytoplasmic Foci 0.0198 0.0001 0.0014 0.0031 0.0001 0 0.0075 0.0001 0 0 0.0069 0 0.0006 0 0 0 0 0
Eisosomes 0.004 0.001 0.0007 0.0031 0.0082 0.0006 0.0059 0.0004 0.0018 0.0003 0.0008 0.0003 0.0006 0.0005 0.0015 0.0006 0.0004 0.0004
Endoplasmic Reticulum 0.0934 0.0633 0.0529 0.2113 0.2446 0.1735 0.1738 0.0972 0.0573 0.1867 0.2552 0.1758 0.1648 0.0945 0.085 0.4271 0.2537 0.1366
Endosome 0.0305 0.0001 0.0013 0.0051 0.0003 0.0003 0.0104 0 0 0 0.0153 0 0.0012 0 0 0 0 0
Golgi 0.0114 0.0002 0.0009 0.0091 0.0004 0.0002 0.0039 0.0001 0.0001 0 0.0344 0.0001 0.0015 0 0 0.0001 0.0001 0
Lipid Particles 0.0038 0.0009 0.0007 0.0019 0.0019 0.0012 0.008 0.0003 0.0009 0.0014 0.003 0.0008 0.0014 0.0007 0.0005 0.0012 0.0014 0.0005
Mitochondria 0.0126 0.0004 0.0006 0.0024 0.0063 0.0203 0.0031 0.0001 0 0 0.0074 0.0001 0.0015 0 0 0 0 0
None 0.0032 0.0002 0.0006 0.0003 0.001 0.0001 0.0068 0.0001 0.0001 0 0.0001 0.0001 0.0006 0.0001 0.0001 0.0001 0 0
Nuclear Periphery 0.0367 0.0021 0.0071 0.0036 0.0027 0.003 0.0161 0.0029 0.0024 0.005 0.0027 0.0022 0.0074 0.0042 0.0027 0.004 0.0031 0.0028
Nucleolus 0.0009 0 0.0004 0 0.0001 0 0.0006 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.0343 0.0002 0.0051 0.0003 0.0002 0.0002 0.0034 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001
Peroxisomes 0.01 0 0.0037 0.0005 0 0 0.0082 0 0 0 0.0021 0 0.0005 0 0 0 0 0
Punctate Nuclear 0.0054 0 0.003 0.0001 0 0 0.0035 0 0 0 0.0001 0 0.0001 0 0 0 0 0
Vacuole 0.0173 0.0069 0.0076 0.0066 0.0078 0.0099 0.0129 0.0045 0.0067 0.0088 0.0056 0.0061 0.0129 0.0066 0.0062 0.0062 0.005 0.0064
Vacuole Periphery 0.0032 0.0001 0.0003 0.0002 0.0004 0.0005 0.0015 0 0 0 0.0003 0.0001 0.0001 0 0 0.0001 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.1989 22.1658 73.6241 51.0242 9.568 12.981 42.0221 72.931 61.757 17.6082
Translational Efficiency 0.9658 1.663 1.2954 1.562 1.5834 0.7289 1.2601 1.2408 1.1479 1.1192

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; specific for spermine; localizes to the plasma membrane; TPO3 has a paralog, TPO2, that arose from the whole genome duplication
Localization
Cell Percentages cell periphery (72%), ER (9%), mixed (17%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Tpo3

Tpo3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tpo3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available