Standard name
Human Ortholog
Description AAA-ATPase involved in multivesicular body (MVB) protein sorting; ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 0 0 0 0 – – – – – – 0 0 0 0.11 0 0
Bud – 0 0 0 0 0 0 0 0 0 0 0.05 – – – – – – 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 – – – – – – 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – – – – –
Cell Periphery – 0 0 0 0 0 0 0 0 0 0 0 – – – – – – 0 0 0 0 0 0
Cytoplasm – 0.09 0 0 0.06 0.06 0 0 0 0 0 0 – – – – – – 0 0 0 0 0 0
Endoplasmic Reticulum – 0 0 0 0 0 0 0 0 0 0 0 – – – – – – 0 0 0 0.08 0 0.07
Endosome – 0.09 0.51 0.36 0.48 0.38 0.46 0.36 0.22 0.2 0.16 0.18 – – – – – – 0.53 0.53 0.65 0.11 0.39 0.36
Golgi – 0 0 0 0 0 0 0 0 0 0 0 – – – – – – 0 0 0 0.37 0.36 0.25
Mitochondria – 0.48 0.22 0.25 0.18 0.41 0.31 0.34 0.6 0.53 0.63 0.64 – – – – – – 0 0 0 0 0 0
Nucleus – 0 0 0 0 0 0 0 0 0 0 0 – – – – – – 0 0 0 0 0 0
Nuclear Periphery – 0 0 0 0 0 0 0 0.05 0 0 0 – – – – – – 0 0 0 0 0.14 0.06
Nucleolus – 0.35 0.25 0.28 0.21 0.26 0.2 0.23 0.19 0.28 0.21 0.21 – – – – – – 0 0.06 0 0 0.05 0
Peroxisomes – 0 0.21 0.19 0.31 0.19 0.27 0.26 0.09 0.15 0.14 0.09 – – – – – – 0 0 0.1 0 0 0
SpindlePole – 0.3 0.34 0.3 0.24 0.21 0.25 0.29 0.14 0.19 0.18 0.18 – – – – – – 0.27 0.19 0.16 0 0 0.09
Vac/Vac Membrane – 0 0 0 0 0 0 0 0 0 0 0 – – – – – – 0 0 0 0.23 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 1 0 1 1 0 1 2 0 0 1 – – – – – – 0 0 2 0 0 0
Bud – 0 3 5 2 2 6 7 2 4 5 10 – – – – – – 0 1 2 0 0 0
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 – – – – – – 0 0 0 0 0 0
Bud Site – 1 0 0 0 0 0 3 0 0 1 0 – – – – – – – – – – – –
Cell Periphery – 0 0 0 0 0 0 0 0 0 0 0 – – – – – – 0 0 0 0 0 0
Cytoplasm – 2 5 6 10 13 13 6 1 4 3 3 – – – – – – 0 1 0 0 0 0
Endoplasmic Reticulum – 0 0 0 0 0 0 0 1 0 1 0 – – – – – – 0 0 0 0 0 0
Endosome – 2 91 51 82 80 139 115 30 33 30 34 – – – – – – 18 39 125 0 1 3
Golgi – 0 0 0 0 0 0 0 0 0 0 0 – – – – – – 0 1 4 1 1 2
Mitochondria – 11 39 35 30 85 92 110 82 86 117 122 – – – – – – 1 2 0 0 0 0
Nucleus – 0 0 0 0 2 0 1 1 2 2 1 – – – – – – 0 1 0 0 0 0
Nuclear Periphery – 0 2 2 3 0 4 5 7 3 0 1 – – – – – – 0 0 1 0 0 0
Nucleolus – 8 44 39 35 55 59 72 26 46 39 41 – – – – – – 0 4 1 0 0 0
Peroxisomes – 1 38 27 53 40 82 84 12 25 26 17 – – – – – – 1 2 20 0 0 0
SpindlePole – 7 60 42 40 44 76 92 19 31 34 35 – – – – – – 9 13 30 0 0 0
Vac/Vac Membrane – 1 5 3 7 8 6 9 4 4 4 8 – – – – – – 0 1 0 0 0 0
Unique Cell Count 23 178 141 170 208 299 319 137 163 186 192 35 74 194 4 4 11
Labelled Cell Count 33 288 210 263 330 477 505 187 238 262 273 35 74 194 4 4 11


Endosome

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 7.5 7.7 7.8 7.9 7.0 7.0 7.0 6.3 5.9 6.4 6.3 – – – – – – – – –
Std Deviation (1e-4) – 1.5 1.4 1.8 2.1 1.7 1.7 1.6 1.4 1.3 1.7 1.4 – – – – – – – – –
Intensity Change (Log2) – – – 0.04 0.06 -0.14 -0.12 -0.14 -0.28 -0.37 -0.27 -0.27 – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 – – – – – –
Bud 0 0 0 0 0 0 0 0 1.8 – – – – – –
Bud Neck 0 0 0 0 0 0 0 0 0 – – – – – –
Bud Site 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – – – – – –
Cytoplasm 0 1.4 1.6 0.9 0 0 0 0 0 – – – – – –
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – – – – – –
Endosome -2.7 -0.5 -2.5 -1.0 -3.3 -5.3 -5.9 -7.1 -6.8 – – – – – –
Golgi 0 0 0 0 0 0 0 0 0 – – – – – –
Mitochondria 0.6 -1.0 4.0 2.1 2.9 6.9 5.9 7.9 8.1 – – – – – –
Nucleus 0 0 0 0 0 0 0 0 0 – – – – – –
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – – – – – –
Nucleolus 0.6 -0.9 0.4 -1.3 -0.5 -1.2 0.7 -0.9 -0.8 – – – – – –
Peroxisomes -0.5 2.1 -0.5 1.5 1.2 -3.0 -1.4 -1.8 -3.4 – – – – – –
SpindlePole -0.7 -2.1 -2.8 -1.9 -1.1 -4.0 -3.1 -3.4 -3.4 – – – – – –
Vacuole 0 0.7 0.6 0 0 0 0 0 0.7 – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0208 0.0005 0.0261 0.0607 0.0155 0.0005 0.0006 0.0001 0.018 0.0001 0.0001 0.0009 – – – – – –
Bud 0.0003 0.0005 0.009 0.0002 0.0008 0.0001 0.0001 0 0.0002 0 0 0 – – – – – –
Bud Neck 0.0014 0.0013 0.0014 0.0003 0.0007 0.0003 0.0002 0.002 0.0007 0.0003 0.0001 0.0002 – – – – – –
Bud Periphery 0.0002 0.0003 0.0119 0.0002 0.0015 0.0001 0.0001 0 0.0003 0 0 0 – – – – – –
Bud Site 0.0027 0.0038 0.0117 0.001 0.0017 0.0003 0.0003 0.0002 0.0011 0.0001 0 0.0001 – – – – – –
Cell Periphery 0.0002 0.0004 0.0005 0 0.0003 0 0 0 0.0002 0 0 0 – – – – – –
Cytoplasm 0.0205 0.0016 0.0067 0.0004 0.0043 0.0003 0.0019 0.0002 0.0008 0.0001 0.0001 0.0001 – – – – – –
Cytoplasmic Foci 0.2471 0.3119 0.2337 0.1632 0.2351 0.2175 0.2359 0.2412 0.1817 0.1744 0.203 0.1754 – – – – – –
Eisosomes 0.0002 0 0.0001 0.0001 0.0002 0 0 0 0.0001 0 0 0 – – – – – –
Endoplasmic Reticulum 0.0007 0.0002 0.0011 0.0001 0.005 0.0181 0.0009 0.0001 0.0004 0 0 0 – – – – – –
Endosome 0.152 0.1673 0.1171 0.2039 0.2385 0.0964 0.0705 0.0826 0.0876 0.0536 0.0315 0.0698 – – – – – –
Golgi 0.0437 0.0608 0.0521 0.1108 0.0608 0.0747 0.0455 0.0379 0.107 0.0343 0.0255 0.0528 – – – – – –
Lipid Particles 0.3625 0.288 0.3367 0.2257 0.2214 0.4466 0.5285 0.4926 0.3689 0.475 0.5573 0.5702 – – – – – –
Mitochondria 0.0054 0.0032 0.0152 0.002 0.0665 0.0035 0.0021 0.0012 0.0119 0.0005 0.0005 0.0012 – – – – – –
None 0.0017 0.0001 0.0012 0.0001 0.0092 0 0.0022 0 0.0003 0 0 0 – – – – – –
Nuclear Periphery 0.0052 0.0002 0.0009 0 0.0087 0.0001 0.011 0.0003 0.0001 0 0 0.0001 – – – – – –
Nucleolus 0.0072 0.007 0.0128 0.0001 0.001 0.0007 0.0016 0.001 0.0138 0.0063 0.0001 0.01 – – – – – –
Nucleus 0.0017 0.001 0.0088 0 0.0013 0.0001 0.0085 0.0001 0.0004 0.0003 0 0.0002 – – – – – –
Peroxisomes 0.1077 0.1096 0.0966 0.2298 0.1023 0.1363 0.0827 0.1335 0.2017 0.2502 0.1778 0.1008 – – – – – –
Punctate Nuclear 0.01 0.0121 0.035 0.0006 0.0051 0.0025 0.0043 0.0015 0.0011 0.0038 0.0032 0.0157 – – – – – –
Vacuole 0.0073 0.0263 0.0186 0.0004 0.0105 0.0014 0.0018 0.0031 0.0026 0.0006 0.0004 0.0016 – – – – – –
Vacuole Periphery 0.0013 0.0038 0.0028 0.0005 0.0096 0.0006 0.0012 0.0024 0.0011 0.0002 0.0002 0.0008 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 31.4899 33.78 63.5011 59.7126 44.6386 45.3203 46.6784 63.5218 64.845 49.7336
Translational Efficiency 0.6779 1.2134 0.9231 1.1313 0.6923 0.4946 1.0732 0.8963 0.8983 0.5766

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

No result is found for the queried gene(s). Please try another query.


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vps4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available