Standard name
Human Ortholog
Description Splicing factor; component of the U4/U6-U5 snRNP complex
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.11 0.1 0.16 0.24 0.17 0.12 0.12 0.13 0.08 0.12 0.08 0.22 0.27 0.26 0.22 0.16 0.13 0 0.11 0.11 0.12 0.09 0.11
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.37 0.43 0.45 0.66 0.49 0.72 0.74 0.76 0.77 0.91 0.76 0.84 0.08 0.06 0.05 0.12 0.08 0.06 0 0 0 0 0 0
Nucleus 0.78 0.48 0.5 0.41 0.42 0.41 0.55 0.53 0.51 0.42 0.51 0.51 0.58 0.55 0.55 0.53 0.62 0.67 0.76 0.63 0.53 0.52 0.46 0.32
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.06
Vac/Vac Membrane 0.1 0.25 0.05 0 0.05 0 0 0 0 0 0 0 0.13 0.17 0.18 0.21 0.18 0.21 0.13 0.16 0.26 0.18 0.24 0.28
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 4
Bud 0 0 0 0 0 3 0 2 1 1 3 1 0 0 1 1 1 0 0 1 0 9 10 23
Bud Neck 0 0 0 0 0 0 0 2 1 0 1 0 0 0 0 1 0 0 0 0 0 0 2 10
Bud Site 1 0 0 0 1 0 3 7 1 4 7 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 4 8 2 16 20 16 23 33 19 12 21 12 112 166 176 61 37 14 4 10 7 48 47 61
Endoplasmic Reticulum 0 0 0 1 0 0 3 0 0 0 0 0 1 3 1 15 16 4 0 0 0 0 1 4
Endosome 0 1 0 0 0 0 0 0 0 0 0 0 14 8 20 7 10 2 2 0 0 5 9 15
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 1 1 1 3 2
Mitochondria 61 32 9 66 41 67 139 202 109 138 128 123 38 36 36 33 18 7 3 1 1 8 16 24
Nucleus 128 36 10 41 35 38 104 140 72 64 86 75 292 339 372 150 141 74 141 64 35 203 241 183
Nuclear Periphery 1 0 0 2 0 0 2 0 0 2 1 3 0 0 0 1 0 0 0 0 0 1 2 2
Nucleolus 12 1 0 0 1 0 2 0 0 0 0 1 2 1 1 0 1 0 2 1 0 6 14 13
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 1 0 1
SpindlePole 1 0 0 0 2 0 2 6 2 0 1 0 0 0 1 2 1 2 1 2 1 21 33 36
Vac/Vac Membrane 17 19 1 1 4 4 1 6 4 4 3 1 64 107 122 58 42 23 24 16 17 72 123 159
Unique Cell Count 165 75 20 100 84 93 189 265 142 152 168 146 503 620 677 282 228 111 187 103 67 393 524 566
Labelled Cell Count 225 97 22 127 104 128 279 398 209 225 251 218 523 661 733 331 267 126 187 103 67 393 524 566


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 4.0 3.6 3.5 3.8 3.3 3.3 3.2 3.4 2.7 3.2 2.8 4.8 4.7 4.7 5.0 5.9 6.0 4.3 4.3 5.0
Std Deviation (1e-4) 0.9 0.7 0.4 1.3 1.4 1.3 1.2 1.1 1.6 0.8 1.3 0.7 1.3 1.5 1.4 1.1 1.3 1.3 1.4 1.1 1.5
Intensity Change (Log2) – – – -0.05 0.07 -0.13 -0.14 -0.16 -0.09 -0.4 -0.17 -0.34 0.43 0.4 0.39 0.49 0.72 0.75 0.26 0.26 0.47


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.3204 3.2445 3.2715 3.3945 3.308 3.3701 0.9379 1.3601 1.3664 1.172 0.9149 1.405 2.1735 2.6555 2.2874 2.5633 2.7023 2.4068
Actin 0.0793 0.0004 0.0083 0.0034 0.0035 0.0027 0.0088 0.0003 0.0119 0.0033 0.0063 0.0023 0.0216 0.0004 0.0013 0.0002 0.0352 0.0004
Bud 0.0022 0.0015 0.0051 0.0178 0.0006 0.0012 0.0002 0.0001 0.001 0.0005 0.0001 0.0001 0.0002 0.0067 0.0004 0.0154 0.0132 0.0001
Bud Neck 0.017 0.0005 0.0026 0.001 0.0019 0.0045 0.0018 0.0005 0.0014 0.0013 0.0007 0.0033 0.002 0.0007 0.0007 0.0005 0.002 0.0027
Bud Periphery 0.0049 0.0001 0.0007 0.0071 0.0013 0.0009 0.0002 0.0001 0.002 0.0009 0.0002 0.0001 0.0002 0.001 0.0001 0.0011 0.0012 0.0001
Bud Site 0.0103 0.0039 0.0017 0.0009 0.001 0.0008 0.0059 0.0021 0.0038 0.004 0.0002 0.0002 0.0007 0.003 0.0006 0.0005 0.0013 0.0002
Cell Periphery 0.001 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0.0002 0.0002 0.0001 0 0 0.0001 0.0001 0 0.0001 0.0002 0
Cytoplasm 0.0302 0.0562 0.0691 0.0427 0.0121 0.0446 0.0283 0.0648 0.0363 0.0373 0.0396 0.0383 0.0448 0.086 0.0365 0.0458 0.095 0.0407
Cytoplasmic Foci 0.0172 0.0015 0.0067 0.0389 0.0167 0.0059 0.013 0.0028 0.0035 0.0037 0.0089 0.0042 0.0154 0.0026 0.0017 0.0037 0.0233 0.0027
Eisosomes 0.0008 0 0.0001 0.0001 0.0001 0.0001 0.0005 0.0002 0.0002 0.0002 0.0001 0.0001 0.0002 0.0001 0 0 0.0002 0
Endoplasmic Reticulum 0.008 0.0018 0.0022 0.0012 0.0039 0.0017 0.0059 0.0022 0.0042 0.001 0.0011 0.002 0.0046 0.0019 0.0014 0.0012 0.0029 0.0009
Endosome 0.0207 0.0022 0.0082 0.0058 0.0711 0.0048 0.0068 0.0019 0.0073 0.0032 0.01 0.0054 0.0075 0.0022 0.002 0.0014 0.0065 0.002
Golgi 0.0067 0.0001 0.0021 0.0021 0.0133 0.005 0.0031 0.0001 0.0025 0.001 0.0033 0.0025 0.0022 0.0001 0.0002 0 0.0009 0.0001
Lipid Particles 0.011 0.0004 0.0047 0.0036 0.0305 0.0033 0.0087 0.0005 0.006 0.0056 0.0279 0.0043 0.0019 0.0003 0.0001 0.0002 0.0017 0.0007
Mitochondria 0.0131 0.0005 0.0016 0.048 0.0145 0.0012 0.0013 0.0005 0.007 0.0031 0.001 0.0013 0.0014 0.0014 0.0006 0.0009 0.0049 0.0005
None 0.0954 0.1446 0.1355 0.207 0.1202 0.0893 0.198 0.3659 0.2518 0.3649 0.2931 0.1961 0.0876 0.2072 0.0926 0.1217 0.2144 0.0572
Nuclear Periphery 0.0507 0.0106 0.014 0.0125 0.0086 0.0085 0.0128 0.0178 0.0164 0.0132 0.0149 0.0107 0.0191 0.0142 0.0125 0.0085 0.0304 0.0072
Nucleolus 0.0154 0.0083 0.0082 0.0074 0.0164 0.0198 0.0075 0.0076 0.0064 0.0068 0.0091 0.0156 0.0064 0.0078 0.0064 0.0053 0.0097 0.0137
Nucleus 0.5439 0.7284 0.6574 0.4895 0.6015 0.7342 0.6496 0.4936 0.6018 0.5148 0.5161 0.6557 0.754 0.6214 0.8138 0.755 0.4559 0.8148
Peroxisomes 0.0175 0.0001 0.0084 0.0049 0.0122 0.0057 0.0226 0.0002 0.0044 0.0073 0.0094 0.0046 0.0011 0.0002 0.0001 0.0001 0.0094 0.0056
Punctate Nuclear 0.0435 0.0359 0.0613 0.1032 0.0652 0.063 0.0228 0.0369 0.0278 0.0265 0.057 0.0519 0.0268 0.0402 0.028 0.0374 0.0868 0.0494
Vacuole 0.0068 0.0025 0.0018 0.001 0.0031 0.0021 0.0016 0.0014 0.003 0.0009 0.0005 0.0009 0.0017 0.0022 0.0007 0.0007 0.0031 0.0009
Vacuole Periphery 0.0044 0.0003 0.0005 0.0016 0.0023 0.0004 0.0004 0.0003 0.0009 0.0003 0.0004 0.0002 0.0006 0.0004 0.0003 0.0002 0.0017 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.9196 4.9389 7.3298 11.5228 12.7916 8.6772 10.5226 13.241 13.3208 10.0708
Translational Efficiency 1.5815 2.3075 1.6209 1.2321 0.7278 1.4183 1.2528 1.1887 1.0479 1.0356

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✔
Standard name
Human Ortholog
Description Splicing factor; component of the U4/U6-U5 snRNP complex
Localization
Cell Percentages nucleus (85%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Prp4

Prp4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Prp4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available