Standard name | |
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Human Ortholog | |
Description | Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 |
Bud | 0 | 0 | 4 | 1 | 0 | 2 | 9 | 16 | 5 | 8 | 3 | 12 | 0 | 3 | 11 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 3 |
Bud Neck | 0 | 0 | 3 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 7 | 9 | 15 | 21 | 16 | 32 | 9 | 20 | 0 | 0 | 10 | 9 | 7 | 8 | 1 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 202 | 179 | 156 | 117 | 225 | 279 | 304 | 333 | 173 | 234 | 149 | 193 | 156 | 230 | 237 | 154 | 126 | 144 | 194 | 179 | 133 | 74 | 182 | 207 |
Endoplasmic Reticulum | 1 | 0 | 1 | 0 | 1 | 4 | 2 | 6 | 1 | 5 | 3 | 5 | 0 | 1 | 1 | 10 | 6 | 10 | 0 | 0 | 2 | 0 | 0 | 2 |
Endosome | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 3 | 1 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 6 | 18 | 9 | 14 | 12 | 55 | 66 | 121 | 94 | 102 | 98 | 135 | 1 | 3 | 6 | 4 | 0 | 0 | 11 | 6 | 11 | 1 | 0 | 5 |
Nucleus | 0 | 0 | 1 | 0 | 0 | 2 | 2 | 2 | 2 | 2 | 3 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 |
Nuclear Periphery | 1 | 2 | 1 | 0 | 0 | 0 | 2 | 2 | 4 | 0 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 1 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 |
Vac/Vac Membrane | 3 | 1 | 0 | 1 | 4 | 2 | 23 | 53 | 18 | 14 | 13 | 13 | 0 | 3 | 2 | 4 | 2 | 5 | 3 | 0 | 1 | 4 | 5 | 3 |
Unique Cell Count | 208 | 187 | 161 | 121 | 233 | 305 | 357 | 451 | 234 | 319 | 230 | 287 | 156 | 238 | 247 | 161 | 131 | 150 | 217 | 192 | 161 | 90 | 201 | 237 |
Labelled Cell Count | 214 | 200 | 175 | 133 | 250 | 354 | 424 | 559 | 314 | 398 | 282 | 384 | 157 | 243 | 271 | 183 | 143 | 171 | 217 | 192 | 161 | 90 | 201 | 237 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.1 | 3.6 | 3.8 | 3.3 | 4.9 | 5.1 | 5.5 | 5.7 | 5.5 | 5.8 | 5.6 | 5.3 | 5.0 | 6.6 | 6.6 | 6.7 | 7.2 | 7.3 | 4.7 | 4.5 | 5.4 |
Std Deviation (1e-4) | 2.3 | 0.8 | 1.5 | 1.0 | 2.1 | 1.7 | 2.1 | 1.9 | 2.1 | 2.2 | 2.1 | 2.3 | 1.4 | 2.5 | 2.7 | 2.1 | 2.5 | 3.5 | 1.2 | 1.5 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.18 | 0.39 | 0.44 | 0.54 | 0.6 | 0.55 | 0.61 | 0.56 | 0.49 | 0.4 | 0.81 | 0.82 | 0.82 | 0.94 | 0.96 | 0.31 | 0.24 | 0.52 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 20.3906 | 29.9745 | 20.8799 | 40.06 | 2.8787 | 25.089 | 1.8039 | 2.7298 | 2.7234 | 1.6608 | 2.3483 | 2.2225 | 2.6896 | 2.855 | 2.1273 | 1.8236 | 2.8721 | 2.3111 |
Actin | 0.0117 | 0.0047 | 0.0115 | 0.0021 | 0.0022 | 0.01 | 0.0308 | 0.0011 | 0.0029 | 0.0003 | 0.0243 | 0.0077 | 0.0311 | 0.0657 | 0.0473 | 0.0012 | 0.0007 | 0.0022 |
Bud | 0.0055 | 0.0172 | 0.0297 | 0.0112 | 0.0022 | 0.0031 | 0.0015 | 0.0036 | 0.0077 | 0.0006 | 0.0032 | 0.0024 | 0.0013 | 0.0008 | 0.0012 | 0.0001 | 0.0003 | 0.003 |
Bud Neck | 0.0017 | 0.0008 | 0.0044 | 0.0013 | 0.0005 | 0.0024 | 0.0064 | 0.0002 | 0.0009 | 0.0001 | 0.0019 | 0.0029 | 0.0012 | 0.0008 | 0.0006 | 0.0002 | 0.0001 | 0.0006 |
Bud Periphery | 0.0134 | 0.0059 | 0.02 | 0.0083 | 0.002 | 0.0027 | 0.0019 | 0.0021 | 0.0046 | 0.0006 | 0.0038 | 0.0025 | 0.0031 | 0.0008 | 0.0011 | 0 | 0.0011 | 0.0049 |
Bud Site | 0.0061 | 0.0229 | 0.02 | 0.0016 | 0.0013 | 0.0006 | 0.0055 | 0.0132 | 0.0049 | 0.0002 | 0.0183 | 0.0018 | 0.0069 | 0.0322 | 0.0031 | 0.0002 | 0.0001 | 0.0002 |
Cell Periphery | 0.0138 | 0.0089 | 0.0122 | 0.0171 | 0.0011 | 0.003 | 0.0002 | 0.0002 | 0.0005 | 0 | 0.0003 | 0.0002 | 0.0015 | 0.0012 | 0.0002 | 0.0001 | 0 | 0.0001 |
Cytoplasm | 0.6109 | 0.7633 | 0.6734 | 0.6092 | 0.7406 | 0.7833 | 0.4176 | 0.6577 | 0.5896 | 0.4388 | 0.5209 | 0.671 | 0.4323 | 0.6502 | 0.5772 | 0.6484 | 0.3906 | 0.59 |
Cytoplasmic Foci | 0.0331 | 0.0205 | 0.0131 | 0.0225 | 0.0292 | 0.0265 | 0.031 | 0.0077 | 0.0097 | 0.0076 | 0.0147 | 0.0146 | 0.0329 | 0.0021 | 0.0052 | 0.0073 | 0.0128 | 0.0268 |
Eisosomes | 0.0004 | 0.0001 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0021 | 0.0002 | 0.0003 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0301 | 0.0027 | 0.0051 | 0.0173 | 0.0048 | 0.0067 | 0.0056 | 0.0017 | 0.0062 | 0.0013 | 0.0022 | 0.0041 | 0.0339 | 0.0013 | 0.0012 | 0.0024 | 0.0014 | 0.0011 |
Endosome | 0.0164 | 0.007 | 0.0059 | 0.0053 | 0.0156 | 0.0115 | 0.0207 | 0.0012 | 0.0051 | 0.0013 | 0.0195 | 0.0036 | 0.031 | 0.0003 | 0.002 | 0.003 | 0.002 | 0.0018 |
Golgi | 0.003 | 0.0011 | 0.0066 | 0.0005 | 0.0019 | 0.0032 | 0.0202 | 0.0002 | 0.0005 | 0.0001 | 0.0044 | 0.0016 | 0.0155 | 0.0004 | 0.0044 | 0.0003 | 0.0009 | 0.0005 |
Lipid Particles | 0.0101 | 0.0008 | 0.0079 | 0.0013 | 0.0103 | 0.0063 | 0.019 | 0.0001 | 0.0005 | 0.0002 | 0.0036 | 0.0011 | 0.0382 | 0.0002 | 0.003 | 0.0004 | 0.0087 | 0.0003 |
Mitochondria | 0.0123 | 0.0007 | 0.0131 | 0.001 | 0.0196 | 0.0089 | 0.0197 | 0.0002 | 0.0085 | 0.0003 | 0.0022 | 0.0021 | 0.034 | 0.0001 | 0.0059 | 0.0002 | 0.0046 | 0.0004 |
None | 0.0943 | 0.0844 | 0.0951 | 0.0581 | 0.0882 | 0.0537 | 0.3801 | 0.3044 | 0.3424 | 0.5436 | 0.3443 | 0.2693 | 0.2905 | 0.2412 | 0.338 | 0.3305 | 0.5521 | 0.3639 |
Nuclear Periphery | 0.0284 | 0.0022 | 0.0045 | 0.016 | 0.0178 | 0.0091 | 0.0036 | 0.0009 | 0.0031 | 0.0007 | 0.0021 | 0.0026 | 0.0073 | 0.0002 | 0.0004 | 0.0012 | 0.0008 | 0.001 |
Nucleolus | 0.002 | 0.0008 | 0.0061 | 0.0008 | 0.0137 | 0.0091 | 0.0006 | 0.0001 | 0.0003 | 0.0001 | 0.0041 | 0.0006 | 0.0008 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 |
Nucleus | 0.009 | 0.0043 | 0.0078 | 0.0087 | 0.0113 | 0.0075 | 0.0138 | 0.0017 | 0.0032 | 0.0015 | 0.0059 | 0.0031 | 0.0021 | 0.0004 | 0.0007 | 0.0015 | 0.0006 | 0.001 |
Peroxisomes | 0.0055 | 0.0013 | 0.0063 | 0.0004 | 0.002 | 0.0017 | 0.017 | 0.0003 | 0.0004 | 0.0002 | 0.0061 | 0.0042 | 0.0276 | 0.0009 | 0.0063 | 0.0002 | 0.0208 | 0.0006 |
Punctate Nuclear | 0.0054 | 0.0036 | 0.0026 | 0.0061 | 0.0096 | 0.0076 | 0.0022 | 0.0023 | 0.0023 | 0.0019 | 0.003 | 0.0027 | 0.0022 | 0.0002 | 0.0012 | 0.0022 | 0.0011 | 0.0007 |
Vacuole | 0.0735 | 0.0437 | 0.0502 | 0.1817 | 0.0116 | 0.0335 | 0.0017 | 0.0008 | 0.0061 | 0.0005 | 0.0139 | 0.0013 | 0.0032 | 0.0009 | 0.0005 | 0.0003 | 0.0005 | 0.0007 |
Vacuole Periphery | 0.0134 | 0.0032 | 0.0044 | 0.0296 | 0.0144 | 0.0096 | 0.0007 | 0.0001 | 0.0007 | 0.0001 | 0.0012 | 0.0004 | 0.0013 | 0 | 0.0001 | 0.0001 | 0.0003 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 16.1526 | 5.5619 | 4.2859 | 9.0833 | 8.0668 | 13.8716 | 8.5976 | 9.2699 | 15.5452 | 12.9258 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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