Standard name | |
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Human Ortholog | |
Description | Ski complex component and TPR protein; mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay; mutations in the human ortholog, TTC37, causes Syndromic diarrhea/Trichohepatoenteric (SD/THE) syndrome |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 4 | 1 | 3 | 3 | 0 | 2 | 1 | 1 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 1 | 0 | 4 | 2 | 4 | 4 | 8 | 7 | 2 | 9 | 7 | 5 | 2 | 0 | 6 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 0 | 2 | 4 | 11 | 13 | 18 | 24 | 13 | 25 | 13 | 11 | 5 | 6 | 4 | 47 | 19 | 32 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 240 | 62 | 134 | 183 | 286 | 263 | 365 | 382 | 167 | 229 | 111 | 154 | 185 | 263 | 297 | 766 | 347 | 678 | 273 | 70 | 136 | 69 | 287 | 284 |
Endoplasmic Reticulum | 4 | 0 | 1 | 1 | 6 | 7 | 22 | 6 | 7 | 10 | 11 | 5 | 1 | 1 | 4 | 30 | 21 | 25 | 8 | 1 | 2 | 1 | 11 | 30 |
Endosome | 1 | 0 | 0 | 0 | 2 | 0 | 2 | 5 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 3 | 0 | 0 | 0 | 0 | 1 | 0 |
Mitochondria | 15 | 5 | 16 | 30 | 13 | 72 | 158 | 206 | 122 | 195 | 110 | 146 | 1 | 2 | 15 | 15 | 7 | 10 | 4 | 1 | 5 | 0 | 1 | 1 |
Nucleus | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 1 | 1 | 0 | 2 | 0 | 2 | 0 | 0 | 8 | 6 | 12 | 1 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 3 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 18 | 0 | 1 | 1 | 6 | 0 | 14 | 57 | 4 | 18 | 4 | 17 | 0 | 1 | 1 | 32 | 8 | 55 | 2 | 1 | 5 | 1 | 8 | 4 |
Unique Cell Count | 271 | 63 | 143 | 191 | 301 | 305 | 487 | 562 | 254 | 381 | 197 | 262 | 190 | 267 | 305 | 818 | 369 | 750 | 294 | 76 | 153 | 74 | 312 | 327 |
Labelled Cell Count | 287 | 67 | 158 | 221 | 331 | 362 | 590 | 695 | 323 | 491 | 262 | 340 | 198 | 274 | 328 | 903 | 410 | 818 | 294 | 76 | 153 | 74 | 312 | 327 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.9 | 9.7 | 6.8 | 7.1 | 7.4 | 6.9 | 6.6 | 6.6 | 5.9 | 6.1 | 5.6 | 5.4 | 11.0 | 10.3 | 9.1 | 12.1 | 12.7 | 13.8 | 10.2 | 11.0 | 11.9 |
Std Deviation (1e-4) | 2.0 | 2.0 | 1.1 | 1.5 | 1.3 | 1.3 | 1.2 | 1.4 | 1.2 | 1.4 | 1.3 | 1.2 | 2.0 | 1.5 | 1.4 | 2.2 | 2.4 | 2.6 | 1.4 | 2.0 | 2.2 |
Intensity Change (Log2) | – | – | – | 0.06 | 0.13 | 0.03 | -0.05 | -0.04 | -0.21 | -0.16 | -0.27 | -0.32 | 0.69 | 0.6 | 0.43 | 0.84 | 0.9 | 1.02 | 0.58 | 0.7 | 0.81 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 1.6 | 1.9 | 2.4 | 2.3 | 0 | 0 | 0 | 0 | 2.2 | 1.9 | 1.6 |
Cytoplasm | 0.9 | 0.6 | -2.3 | -4.9 | -6.2 | -6.2 | -7.4 | -7.6 | -7.4 | 1.6 | 2.6 | 1.9 | 0 | 0.1 | -1.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 2.1 | 0 | 0 | 0 | 2.4 | 0 | 0 | 0 | 0 | 0 | 2.5 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1.2 | -2.7 | 3.1 | 5.0 | 5.9 | 7.4 | 8.3 | 8.4 | 8.7 | -4.4 | -4.9 | -2.4 | 0 | -4.6 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 3.7 | 0 | 2.2 | 0 | 2.7 | 0 | 0 | 0 | 0 | 0 | 3.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.9818 | 8.6448 | 6.5878 | 5.9502 | 7.3369 | 7.6543 | 7.5928 | 11.7008 | 10.8959 | 10.4194 | 9.7838 | 10.4821 | 1.8078 | 6.2907 | 5.6112 | 6.4179 | 5.4253 | 5.9462 |
Actin | 0.0503 | 0.0186 | 0.0231 | 0.0024 | 0.0001 | 0.0004 | 0.0104 | 0.0026 | 0.004 | 0.0003 | 0.0003 | 0.0008 | 0.0335 | 0.0015 | 0.0012 | 0.0001 | 0.0002 | 0.0017 |
Bud | 0.0011 | 0.0006 | 0.0024 | 0.0011 | 0.0004 | 0.0002 | 0.0008 | 0.0003 | 0.0005 | 0.0011 | 0.0008 | 0.0016 | 0.0005 | 0.0007 | 0.0011 | 0.0008 | 0.0002 | 0.0005 |
Bud Neck | 0.0028 | 0.0005 | 0.0014 | 0.0008 | 0.0004 | 0.0016 | 0.0007 | 0.0006 | 0.0013 | 0.0008 | 0.0007 | 0.001 | 0.0006 | 0.0002 | 0.0003 | 0.0004 | 0.0003 | 0.0023 |
Bud Periphery | 0.0021 | 0.0004 | 0.0031 | 0.0026 | 0 | 0.0001 | 0.0006 | 0.0002 | 0.0003 | 0.0003 | 0.0005 | 0.0012 | 0.0005 | 0.0005 | 0.0002 | 0.0001 | 0 | 0.0003 |
Bud Site | 0.0209 | 0.0019 | 0.0037 | 0.0007 | 0.0001 | 0.0001 | 0.0063 | 0.0026 | 0.0015 | 0.0003 | 0.0007 | 0.0003 | 0.0013 | 0.0014 | 0.0009 | 0.0002 | 0.0001 | 0.0001 |
Cell Periphery | 0.0051 | 0.0006 | 0.0015 | 0.0005 | 0 | 0.0001 | 0.0005 | 0.0005 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0005 | 0.0008 | 0.0002 | 0 | 0 | 0.0002 |
Cytoplasm | 0.5709 | 0.8241 | 0.8109 | 0.5887 | 0.9656 | 0.8104 | 0.7732 | 0.9523 | 0.9031 | 0.9189 | 0.7987 | 0.893 | 0.3317 | 0.8931 | 0.933 | 0.9225 | 0.9615 | 0.8414 |
Cytoplasmic Foci | 0.0252 | 0.005 | 0.0118 | 0.0155 | 0.0071 | 0.0071 | 0.0192 | 0.0058 | 0.0138 | 0.01 | 0.0116 | 0.012 | 0.0581 | 0.0158 | 0.0178 | 0.0165 | 0.0096 | 0.0205 |
Eisosomes | 0.0004 | 0.0002 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0004 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.1094 | 0.029 | 0.0316 | 0.0093 | 0.0118 | 0.133 | 0.0485 | 0.0049 | 0.0127 | 0.0069 | 0.0167 | 0.027 | 0.0144 | 0.0103 | 0.0043 | 0.0015 | 0.0039 | 0.0436 |
Endosome | 0.0542 | 0.0154 | 0.0366 | 0.1372 | 0.0101 | 0.0313 | 0.0659 | 0.0089 | 0.0314 | 0.0336 | 0.0623 | 0.0346 | 0.1156 | 0.0212 | 0.0312 | 0.0305 | 0.0169 | 0.0588 |
Golgi | 0.0148 | 0.0036 | 0.0101 | 0.0415 | 0.0009 | 0.0028 | 0.0075 | 0.0013 | 0.0073 | 0.0011 | 0.002 | 0.0032 | 0.0133 | 0.0048 | 0.0019 | 0.0005 | 0.0006 | 0.0116 |
Lipid Particles | 0.0114 | 0.0003 | 0.0032 | 0.0639 | 0 | 0 | 0.0093 | 0.0009 | 0.0046 | 0 | 0.0002 | 0.0002 | 0.2142 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 |
Mitochondria | 0.0627 | 0.0024 | 0.0105 | 0.0933 | 0.0008 | 0.0039 | 0.0064 | 0.0018 | 0.0022 | 0.001 | 0.0015 | 0.0132 | 0.0077 | 0.0047 | 0.0004 | 0.0002 | 0.0005 | 0.0118 |
None | 0.0061 | 0.0004 | 0.0012 | 0.001 | 0.0003 | 0.0006 | 0.0026 | 0.0009 | 0.0008 | 0.0014 | 0.0006 | 0.0008 | 0.0285 | 0.0401 | 0.001 | 0.0011 | 0.0016 | 0.0013 |
Nuclear Periphery | 0.0079 | 0.0014 | 0.004 | 0.0133 | 0.0002 | 0.0026 | 0.0077 | 0.0015 | 0.0018 | 0.0019 | 0.0064 | 0.0025 | 0.1101 | 0.0008 | 0.0006 | 0.0015 | 0.001 | 0.0009 |
Nucleolus | 0.0004 | 0.0048 | 0.0069 | 0.0003 | 0 | 0 | 0.0011 | 0.0025 | 0 | 0 | 0.0027 | 0 | 0.0053 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.0027 | 0.085 | 0.0164 | 0.0015 | 0.0003 | 0.0012 | 0.0045 | 0.0042 | 0.0012 | 0.0019 | 0.0153 | 0.0016 | 0.0245 | 0.0006 | 0.0007 | 0.0019 | 0.0012 | 0.001 |
Peroxisomes | 0.0036 | 0.0001 | 0.0053 | 0.0009 | 0 | 0 | 0.0034 | 0.0001 | 0.0025 | 0.0001 | 0.0001 | 0.0001 | 0.0054 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Punctate Nuclear | 0.0045 | 0.0001 | 0.0004 | 0.0013 | 0 | 0.0001 | 0.0007 | 0.0004 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0224 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 |
Vacuole | 0.0355 | 0.0048 | 0.0126 | 0.0143 | 0.001 | 0.0028 | 0.0244 | 0.0063 | 0.009 | 0.0169 | 0.0617 | 0.0039 | 0.0066 | 0.0026 | 0.0037 | 0.0185 | 0.0015 | 0.0024 |
Vacuole Periphery | 0.0079 | 0.0009 | 0.0031 | 0.01 | 0.0007 | 0.0018 | 0.0063 | 0.0013 | 0.0014 | 0.0035 | 0.0169 | 0.0027 | 0.0049 | 0.0007 | 0.0011 | 0.0035 | 0.0006 | 0.0014 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 26.6932 | 19.5027 | 26.9006 | 32.0061 | 25.1247 | 23.8567 | 27.1428 | 30.7675 | 29.3827 | 36.5702 |
Translational Efficiency | 1.2077 | 1.0967 | 0.8756 | 0.8056 | 1.1184 | 1.1627 | 0.9079 | 0.6335 | 0.7621 | 0.7753 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✔ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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