Standard name
Human Ortholog
Description Ski complex component and TPR protein; mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay; mutations in the human ortholog, TTC37, causes Syndromic diarrhea/Trichohepatoenteric (SD/THE) syndrome

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0.05 0.07 0.07 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0
Cytoplasm 0.89 0.98 0.94 0.96 0.95 0.86 0.75 0.68 0.66 0.6 0.56 0.59 0.97 0.99 0.97 0.94 0.94 0.9 0.93 0.92 0.89 0.94 0.92 0.87
Endoplasmic Reticulum 0 0 0 0 0 0 0.05 0 0 0 0.06 0 0 0 0 0 0.06 0 0 0 0 0 0 0.09
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0.08 0.11 0.16 0 0.24 0.32 0.37 0.48 0.51 0.56 0.56 0 0 0.05 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.07 0 0 0 0 0 0 0.1 0 0.05 0 0.06 0 0 0 0 0 0.07 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 0 3 4 1 3 3 0 2 1 1 1 1 2 0 0 0 0 0 0
Bud 1 0 4 2 4 4 8 7 2 9 7 5 2 0 6 1 1 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0 0
Cell Periphery 3 0 2 4 11 13 18 24 13 25 13 11 5 6 4 47 19 32 0 0 0 0 0 0
Cytoplasm 240 62 134 183 286 263 365 382 167 229 111 154 185 263 297 766 347 678 273 70 136 69 287 284
Endoplasmic Reticulum 4 0 1 1 6 7 22 6 7 10 11 5 1 1 4 30 21 25 8 1 2 1 11 30
Endosome 1 0 0 0 2 0 2 5 1 1 0 0 0 0 0 1 0 1 0 0 0 0 0 0
Golgi 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 3 0 0 0 0 1 0
Mitochondria 15 5 16 30 13 72 158 206 122 195 110 146 1 2 15 15 7 10 4 1 5 0 1 1
Nucleus 0 0 0 0 2 1 0 1 1 0 2 0 2 0 0 8 6 12 1 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 1 0 2 3 0 1 2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1 0 0 0 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 18 0 1 1 6 0 14 57 4 18 4 17 0 1 1 32 8 55 2 1 5 1 8 4
Unique Cell Count 271 63 143 191 301 305 487 562 254 381 197 262 190 267 305 818 369 750 294 76 153 74 312 327
Labelled Cell Count 287 67 158 221 331 362 590 695 323 491 262 340 198 274 328 903 410 818 294 76 153 74 312 327


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.9 9.7 6.8 7.1 7.4 6.9 6.6 6.6 5.9 6.1 5.6 5.4 11.0 10.3 9.1 12.1 12.7 13.8 10.2 11.0 11.9
Std Deviation (1e-4) 2.0 2.0 1.1 1.5 1.3 1.3 1.2 1.4 1.2 1.4 1.3 1.2 2.0 1.5 1.4 2.2 2.4 2.6 1.4 2.0 2.2
Intensity Change (Log2) 0.06 0.13 0.03 -0.05 -0.04 -0.21 -0.16 -0.27 -0.32 0.69 0.6 0.43 0.84 0.9 1.02 0.58 0.7 0.81


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 1.6 1.9 2.4 2.3 0 0 0 0 2.2 1.9 1.6
Cytoplasm 0.9 0.6 -2.3 -4.9 -6.2 -6.2 -7.4 -7.6 -7.4 1.6 2.6 1.9 0 0.1 -1.3
Endoplasmic Reticulum 0 0 0 2.1 0 0 0 2.4 0 0 0 0 0 2.5 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.2 -2.7 3.1 5.0 5.9 7.4 8.3 8.4 8.7 -4.4 -4.9 -2.4 0 -4.6 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 3.7 0 2.2 0 2.7 0 0 0 0 0 3.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.9818 8.6448 6.5878 5.9502 7.3369 7.6543 7.5928 11.7008 10.8959 10.4194 9.7838 10.4821 1.8078 6.2907 5.6112 6.4179 5.4253 5.9462
Actin 0.0503 0.0186 0.0231 0.0024 0.0001 0.0004 0.0104 0.0026 0.004 0.0003 0.0003 0.0008 0.0335 0.0015 0.0012 0.0001 0.0002 0.0017
Bud 0.0011 0.0006 0.0024 0.0011 0.0004 0.0002 0.0008 0.0003 0.0005 0.0011 0.0008 0.0016 0.0005 0.0007 0.0011 0.0008 0.0002 0.0005
Bud Neck 0.0028 0.0005 0.0014 0.0008 0.0004 0.0016 0.0007 0.0006 0.0013 0.0008 0.0007 0.001 0.0006 0.0002 0.0003 0.0004 0.0003 0.0023
Bud Periphery 0.0021 0.0004 0.0031 0.0026 0 0.0001 0.0006 0.0002 0.0003 0.0003 0.0005 0.0012 0.0005 0.0005 0.0002 0.0001 0 0.0003
Bud Site 0.0209 0.0019 0.0037 0.0007 0.0001 0.0001 0.0063 0.0026 0.0015 0.0003 0.0007 0.0003 0.0013 0.0014 0.0009 0.0002 0.0001 0.0001
Cell Periphery 0.0051 0.0006 0.0015 0.0005 0 0.0001 0.0005 0.0005 0.0002 0.0001 0.0003 0.0001 0.0005 0.0008 0.0002 0 0 0.0002
Cytoplasm 0.5709 0.8241 0.8109 0.5887 0.9656 0.8104 0.7732 0.9523 0.9031 0.9189 0.7987 0.893 0.3317 0.8931 0.933 0.9225 0.9615 0.8414
Cytoplasmic Foci 0.0252 0.005 0.0118 0.0155 0.0071 0.0071 0.0192 0.0058 0.0138 0.01 0.0116 0.012 0.0581 0.0158 0.0178 0.0165 0.0096 0.0205
Eisosomes 0.0004 0.0002 0.0002 0 0 0 0.0001 0 0 0 0 0 0.0004 0 0 0 0 0
Endoplasmic Reticulum 0.1094 0.029 0.0316 0.0093 0.0118 0.133 0.0485 0.0049 0.0127 0.0069 0.0167 0.027 0.0144 0.0103 0.0043 0.0015 0.0039 0.0436
Endosome 0.0542 0.0154 0.0366 0.1372 0.0101 0.0313 0.0659 0.0089 0.0314 0.0336 0.0623 0.0346 0.1156 0.0212 0.0312 0.0305 0.0169 0.0588
Golgi 0.0148 0.0036 0.0101 0.0415 0.0009 0.0028 0.0075 0.0013 0.0073 0.0011 0.002 0.0032 0.0133 0.0048 0.0019 0.0005 0.0006 0.0116
Lipid Particles 0.0114 0.0003 0.0032 0.0639 0 0 0.0093 0.0009 0.0046 0 0.0002 0.0002 0.2142 0.0001 0.0001 0 0 0.0001
Mitochondria 0.0627 0.0024 0.0105 0.0933 0.0008 0.0039 0.0064 0.0018 0.0022 0.001 0.0015 0.0132 0.0077 0.0047 0.0004 0.0002 0.0005 0.0118
None 0.0061 0.0004 0.0012 0.001 0.0003 0.0006 0.0026 0.0009 0.0008 0.0014 0.0006 0.0008 0.0285 0.0401 0.001 0.0011 0.0016 0.0013
Nuclear Periphery 0.0079 0.0014 0.004 0.0133 0.0002 0.0026 0.0077 0.0015 0.0018 0.0019 0.0064 0.0025 0.1101 0.0008 0.0006 0.0015 0.001 0.0009
Nucleolus 0.0004 0.0048 0.0069 0.0003 0 0 0.0011 0.0025 0 0 0.0027 0 0.0053 0 0 0 0 0
Nucleus 0.0027 0.085 0.0164 0.0015 0.0003 0.0012 0.0045 0.0042 0.0012 0.0019 0.0153 0.0016 0.0245 0.0006 0.0007 0.0019 0.0012 0.001
Peroxisomes 0.0036 0.0001 0.0053 0.0009 0 0 0.0034 0.0001 0.0025 0.0001 0.0001 0.0001 0.0054 0.0001 0.0001 0.0001 0.0001 0.0001
Punctate Nuclear 0.0045 0.0001 0.0004 0.0013 0 0.0001 0.0007 0.0004 0.0003 0.0001 0.0001 0.0001 0.0224 0.0001 0.0001 0.0002 0.0002 0.0001
Vacuole 0.0355 0.0048 0.0126 0.0143 0.001 0.0028 0.0244 0.0063 0.009 0.0169 0.0617 0.0039 0.0066 0.0026 0.0037 0.0185 0.0015 0.0024
Vacuole Periphery 0.0079 0.0009 0.0031 0.01 0.0007 0.0018 0.0063 0.0013 0.0014 0.0035 0.0169 0.0027 0.0049 0.0007 0.0011 0.0035 0.0006 0.0014

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 26.6932 19.5027 26.9006 32.0061 25.1247 23.8567 27.1428 30.7675 29.3827 36.5702
Translational Efficiency 1.2077 1.0967 0.8756 0.8056 1.1184 1.1627 0.9079 0.6335 0.7621 0.7753

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Ski complex component and TPR protein; mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay; mutations in the human ortholog, TTC37, causes Syndromic diarrhea/Trichohepatoenteric (SD/THE) syndrome
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-5

Ski3

Ski3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ski3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available