Standard name
Human Ortholog
Description Mitochondrial protein required for translation of the COB mRNA; forms a complex with Cbp3p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex; Cbp3p-Cbp6p complex is sequestered if assembly of Complex III is blocked, downregulating COB mRNA translation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0.06 0.05
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.05 0 0 0 0 – 0 0 0 0 0 0 0 0 0.12 0.12 0.16 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0.08 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0.1 0.06 0.2 0.46 0.39 0.36 0.05 0.1 0 0.08 0.13 0.11
Mitochondria 0.86 0.91 0.97 0.97 0.96 0.94 – 0.93 0.95 0.94 0.94 0.95 0.9 0.94 0.84 0.08 0.08 0.05 0.82 0.81 0.88 0.78 0.7 0.67
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0
Nuclear Periphery 0 0 0.06 0 0 0.13 – 0.08 0.16 0.12 0.12 0.1 0 0 0 0 0.05 0 0 0 0 0 0 0
Nucleolus 0 0 0.05 0 0 0.13 – 0.16 0.12 0.13 0.11 0.09 0 0 0 0.06 0.09 0.07 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0.06 0.11 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.27 0.15 0.06 0 0 0.05 – 0.08 0.06 0 0 0 0.13 0.08 0.09 0.27 0.23 0.18 0.07 0 0 0 0 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 4 0 0 0 0 0 – 0 0 0 2 0 1 1 4 8 3 1 7 2 1 6 14 11
Bud 3 0 2 1 5 4 – 6 6 7 1 7 0 1 1 4 1 5 2 0 0 1 1 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 1 – 2 1 3 2 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 14 13 5 1 2 2 – 0 2 1 2 1 4 0 6 17 12 16 2 1 0 0 0 0
Cytoplasm 1 1 1 2 2 3 – 2 2 4 0 1 1 3 1 7 8 5 0 0 0 0 0 0
Endoplasmic Reticulum 6 2 0 0 2 3 – 1 0 3 1 1 2 1 2 1 1 5 3 1 0 0 3 1
Endosome 4 0 0 0 1 0 – 0 0 0 1 1 3 0 3 1 1 2 6 2 1 2 4 4
Golgi 24 5 1 0 1 0 – 0 0 1 1 0 22 11 41 66 39 36 37 27 7 15 32 27
Mitochondria 578 230 180 114 122 228 – 270 264 302 209 276 194 169 170 11 8 5 575 215 156 144 180 167
Nucleus 3 1 1 0 0 2 – 2 0 0 0 0 1 0 1 2 1 8 1 0 0 0 0 1
Nuclear Periphery 29 9 12 1 5 31 – 24 45 38 27 29 7 7 4 2 5 3 5 0 1 1 0 0
Nucleolus 30 9 9 5 4 31 – 46 33 42 25 26 9 5 5 8 9 7 1 1 1 0 1 2
Peroxisomes 3 3 2 1 1 0 – 1 0 0 0 0 3 6 4 8 6 11 6 4 4 6 7 7
SpindlePole 1 0 0 0 1 1 – 0 1 1 1 1 0 0 0 2 0 0 1 0 0 0 0 0
Vac/Vac Membrane 178 37 12 3 4 11 – 23 17 13 10 7 28 15 19 39 23 18 51 8 0 5 7 20
Unique Cell Count 670 254 185 117 127 243 290 278 320 223 290 216 180 202 144 99 100 706 268 177 186 256 249
Labelled Cell Count 878 310 225 128 150 317 377 371 415 282 351 275 219 261 176 117 122 706 268 177 186 256 249


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.4 14.4 6.6 9.0 9.6 8.4 – 8.1 7.8 8.1 8.1 8.0 10.7 10.9 10.5 13.2 12.3 11.9 11.8 13.0 12.6
Std Deviation (1e-4) 2.1 2.8 1.1 1.8 1.8 1.8 – 1.8 1.9 2.0 2.2 1.9 1.8 2.1 1.7 3.2 2.7 2.7 2.6 2.6 2.8
Intensity Change (Log2) – – – 0.44 0.53 0.34 – 0.28 0.24 0.28 0.28 0.27 0.68 0.72 0.67 0.99 0.88 0.84 0.83 0.97 0.93


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0.2 3.3 3.2 4.1
Cytoplasm 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 4.1 3.0 6.2 10.1 9.0 8.5
Mitochondria 0.1 -0.6 -1.7 – -2.0 -1.2 -1.5 -1.7 -1.2 -3.0 -1.6 -4.4 -16.3 -15.1 -15.6
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -2.3 -1.0 2.1 – 0.7 3.1 2.0 1.9 1.3 -1.5 -1.1 -2.2 -2.3 -0.5 -1.3
Nucleolus -0.2 -0.7 2.8 – 3.7 2.6 3.0 2.3 1.7 -0.3 -1.0 -1.3 0.3 1.4 0.7
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.5 -1.3 -0.9 – 0.6 -0.2 -1.2 -0.9 -2.2 2.2 0.7 1.1 5.1 4.1 3.0