Standard name
Human Ortholog
Description Essential protein involved in vesicle-mediated ER to Golgi transport; binds membranes and functions during vesicle docking to the Golgi; required for assembly of the ER-to-Golgi SNARE complex
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.07 0.05 0.12 0 0.06 0 – 0 0 0 0 0 0.13 0.08 0 0.15 0.22 0.18 0 0.11 0 0 0.12 0.12
Bud 0 0 0.09 0.11 0.09 0.09 – 0.11 0.09 0.12 0.13 0.11 0 0.06 0 0 0.09 0.07 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0.05 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0.05 0 0 0 0 0 – 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.09 0.13 0 0 0.15 0 – 0.08 0 0 0 0 0.07 0.08 0.22 0.12 0.13 0.17 0.15 0.12 0.28 0.14 0.13 0.12
Golgi 0.23 0.29 0.05 0 0.08 0 – 0 0 0 0 0 0.55 0.25 0.29 0.57 0.57 0.56 0.59 0.63 0.42 0.65 0.59 0.62
Mitochondria 0.86 0.84 1.0 0.86 0.76 0.82 – 0.68 0.8 0.81 0.83 0.83 0.75 0.7 0.52 0.09 0.07 0.11 0 0 0.05 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.05 0 0.05 0.28 0.3 0.26 – 0.32 0.2 0.24 0.19 0.27 0.06 0.32 0.32 0.08 0.1 0.1 0.15 0.09 0.24 0.17 0.13 0.08
SpindlePole 0 0 0 0.05 0 0.08 – 0.14 0.14 0.12 0.12 0.1 0 0 0.05 0.12 0.09 0.09 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 15 2 8 12 24 8 – 20 7 12 8 14 39 35 14 28 39 34 9 6 0 1 33 45
Bud 5 0 6 35 36 46 – 94 45 72 63 76 11 30 17 8 16 13 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 1 1 4 – 9 8 12 10 22 0 0 1 1 1 0 – – – – – –
Cell Periphery 0 0 1 0 3 1 – 2 3 3 2 0 0 0 0 1 0 4 0 0 0 0 0 0
Cytoplasm 4 1 0 8 6 6 – 9 9 1 6 4 6 11 16 10 9 12 0 0 0 0 0 0
Endoplasmic Reticulum 10 1 0 0 1 1 – 1 0 1 0 1 20 2 0 5 4 5 0 0 0 0 1 2
Endosome 19 5 0 9 57 17 – 72 20 26 7 13 22 39 112 23 23 32 34 7 23 10 36 46
Golgi 49 11 3 5 33 1 – 3 0 6 1 1 162 118 145 104 100 106 136 35 34 51 170 231
Mitochondria 181 32 64 282 297 403 – 596 403 484 388 599 223 326 263 16 13 20 9 0 4 1 6 7
Nucleus 0 0 0 1 0 0 – 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 1 3 – 0 3 3 2 2 0 0 2 0 1 0 1 0 0 0 0 0
Nucleolus 3 1 0 4 1 10 – 42 14 16 12 18 0 4 3 2 2 1 0 0 0 0 0 0
Peroxisomes 11 1 3 93 118 129 – 283 98 145 90 192 17 149 164 15 17 19 33 5 20 13 36 31
SpindlePole 4 0 0 15 17 40 – 127 68 75 54 74 2 14 24 22 16 18 1 0 0 0 0 2
Vac/Vac Membrane 1 1 0 1 1 4 – 1 5 3 2 1 4 0 6 12 5 7 1 1 0 0 0 2
Unique Cell Count 210 38 64 329 390 490 881 502 600 468 720 296 464 505 184 176 190 232 57 83 79 288 375
Labelled Cell Count 302 55 85 466 596 673 1259 684 859 645 1018 506 728 767 247 247 271 232 57 83 79 288 375


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.3 10.6 5.4 6.4 6.9 5.8 – 5.7 5.3 5.5 5.2 5.1 9.4 9.7 9.8 12.8 13.1 12.9 8.1 9.5 9.7
Std Deviation (1e-4) 1.4 1.8 0.8 1.1 1.0 0.8 – 1.0 1.4 1.0 0.9 1.0 1.2 1.6 1.8 2.8 3.1 2.5 1.3 1.6 1.5
Intensity Change (Log2) – – – 0.26 0.37 0.11 – 0.09 -0.03 0.04 -0.06 -0.07 0.8 0.86 0.87 1.25 1.29 1.27 0.59 0.82 0.85


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0.1 -1.4 0 0.5 1.7 1.0
Bud 0.3 0 0 – 0.3 -0.1 0.6 0.9 0.3 0 0 0 0 -0.1 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 3.3 0 – 0 0 0 0 0 0 0 4.2 3.0 3.0 3.5
Golgi 0 1.0 0 – 0 0 0 0 0 7.3 3.7 4.1 7.2 7.2 7.1
Mitochondria -3.2 -4.4 -3.7 – -5.4 -3.9 -3.9 -3.6 -3.6 -4.4 -5.1 -7.3 -13.5 -13.6 -13.2
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 4.0 4.3 3.8 – 4.6 2.9 3.6 2.9 3.9 0 4.5 4.6 0 1.2 1.3
SpindlePole 0 0 0 – 3.3 3.1 3.0 2.9 2.7 0 0 0 2.9 0 0
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0