Standard name | |
---|---|
Human Ortholog | |
Description | Sorting nexin family member; required for the cytoplasm-to-vacuole targeting (Cvt) pathway and for endosomal sorting; has a Phox homology domain that binds phosphatidylinositol-3-phosphate; interacts with Snx4p; potential Cdc28p substrate |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 7 | 8 | 5 | 6 | 5 | 1 | 8 | 15 | 3 | 7 | 7 | 5 | 6 | 5 | 0 | 20 | 9 | 18 | 6 | 5 | 3 | 0 | 1 | 2 |
Bud | 7 | 2 | 2 | 1 | 2 | 7 | 9 | 11 | 13 | 19 | 8 | 10 | 2 | 0 | 1 | 2 | 0 | 3 | 1 | 1 | 1 | 0 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 1 | 2 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 0 | 1 | 0 | 0 | 1 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 58 | 50 | 39 | 25 | 30 | 26 | 48 | 43 | 30 | 40 | 31 | 30 | 23 | 49 | 79 | 36 | 36 | 46 | 27 | 13 | 12 | 11 | 13 | 19 |
Endoplasmic Reticulum | 6 | 9 | 2 | 1 | 2 | 5 | 4 | 3 | 2 | 1 | 1 | 0 | 3 | 3 | 1 | 6 | 8 | 5 | 5 | 2 | 2 | 0 | 2 | 2 |
Endosome | 157 | 109 | 48 | 49 | 66 | 51 | 68 | 44 | 30 | 42 | 28 | 42 | 60 | 69 | 71 | 95 | 75 | 135 | 223 | 157 | 86 | 78 | 100 | 95 |
Golgi | 0 | 5 | 0 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 13 | 10 | 7 | 2 | 6 | 1 | 0 | 1 | 0 |
Mitochondria | 35 | 2 | 1 | 10 | 3 | 21 | 25 | 27 | 58 | 59 | 73 | 61 | 0 | 0 | 0 | 17 | 8 | 11 | 20 | 5 | 24 | 10 | 13 | 9 |
Nucleus | 0 | 1 | 1 | 0 | 1 | 1 | 2 | 4 | 1 | 0 | 4 | 2 | 1 | 0 | 0 | 2 | 2 | 0 | 0 | 1 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 2 | 3 | 0 | 12 | 6 | 5 | 9 | 13 | 6 | 13 | 12 | 10 | 0 | 1 | 4 | 11 | 7 | 10 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 7 | 2 | 1 | 5 | 7 | 5 | 9 | 2 | 3 | 3 | 4 | 5 | 2 | 3 | 9 | 1 | 1 | 6 | 0 | 1 | 0 | 0 | 0 | 0 |
SpindlePole | 45 | 24 | 19 | 67 | 70 | 31 | 72 | 62 | 18 | 49 | 18 | 50 | 23 | 30 | 30 | 61 | 57 | 76 | 13 | 8 | 6 | 10 | 8 | 13 |
Vac/Vac Membrane | 19 | 18 | 11 | 8 | 11 | 3 | 9 | 4 | 6 | 8 | 2 | 6 | 11 | 7 | 8 | 35 | 31 | 28 | 12 | 14 | 14 | 9 | 21 | 9 |
Unique Cell Count | 272 | 183 | 111 | 146 | 164 | 126 | 201 | 176 | 132 | 182 | 147 | 171 | 105 | 135 | 163 | 212 | 180 | 257 | 319 | 224 | 162 | 127 | 171 | 158 |
Labelled Cell Count | 343 | 233 | 129 | 187 | 205 | 156 | 269 | 229 | 172 | 243 | 191 | 225 | 132 | 167 | 205 | 301 | 246 | 347 | 319 | 224 | 162 | 127 | 171 | 158 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.5 | 7.4 | 7.1 | 6.6 | 7.7 | 6.6 | 6.6 | 7.0 | 5.6 | 6.1 | 5.7 | 6.1 | 7.4 | 7.5 | 7.6 | 10.5 | 11.8 | 9.8 | 6.6 | 6.9 | 7.3 |
Std Deviation (1e-4) | 0.7 | 0.9 | 1.6 | 1.6 | 1.8 | 1.7 | 1.4 | 1.5 | 1.2 | 1.2 | 1.2 | 1.6 | 1.2 | 1.1 | 1.3 | 2.1 | 3.4 | 2.4 | 1.5 | 1.2 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.11 | 0.11 | -0.11 | -0.11 | -0.03 | -0.36 | -0.22 | -0.32 | -0.22 | 0.06 | 0.07 | 0.1 | 0.56 | 0.72 | 0.46 | -0.11 | -0.04 | 0.04 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 1.3 | 0 | 0 | 0.1 | 0 | 0.4 | 0 | 0 | 1.6 | 0.2 | 0.9 |
Bud | 0 | 0 | 0 | 0 | 1.8 | 2.6 | 2.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -3.3 | -3.2 | -2.5 | -2.1 | -2.0 | -2.1 | -2.5 | -2.5 | -3.4 | -2.1 | 0.2 | 2.2 | -3.7 | -2.9 | -3.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -1.6 | -0.5 | -0.4 | -1.6 | -3.2 | -3.4 | -3.6 | -4.2 | -3.3 | 2.0 | 1.2 | 0.1 | 0.3 | -0.3 | 1.6 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 4.2 | 3.5 | 4.0 | 7.8 | 6.5 | 8.6 | 6.9 | 0 | 0 | 0 | 2.6 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 3.1 | 0 | 0 | 0 | 2.9 | 0 | 0 | 3.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 4.8 | 4.4 | 1.4 | 3.5 | 3.3 | -0.8 | 1.9 | -1.1 | 2.3 | 0.9 | 1.0 | 0.3 | 2.3 | 2.7 | 2.5 |
Vacuole | -1.3 | -1.0 | -2.5 | -1.9 | -2.8 | -1.6 | -1.9 | -3.1 | -2.2 | 0.1 | -1.4 | -1.6 | 1.6 | 1.7 | 0.3 |