Standard name
Human Ortholog
Description Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; interacts with a highly conserved 40-residue sequence of Snf2p; relocates to the cytosol under hypoxic conditions
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0.06 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0.05 0 – 0 0 0.05 0 0 0.09 0.15 0.06 0.07 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.15 0.23 0.05 0.07 0 0.45 0.06 – 0.3 0.23 0.3 0.28 0 0 0 0.09 0.07 0.08 0 0 0 0 0 0
Nucleus 0.88 0.79 0.92 0.89 0.89 0.78 0.87 – 0.78 0.82 0.72 0.82 0.93 0.88 0.81 0.86 0.84 0.8 0.72 0.74 0.63 0.65 0.65 0.61
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.07 0 0 0 0 0 0 – 0.07 0.09 0.13 0.18 0 0 0 0 0 0 0.12 0.11 0.14 0.21 0.19 0.2
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.07 0.06 0 0 0 – 0 0 0 0 0 0 0 0.05 0.06 0.05 0 0 0.06 0 0.05 0.05
Vac/Vac Membrane 0 0.06 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.05 0.09 0.1 0.07 0.07 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 1 1 1 4 – 4 6 1 0 0 1 0 3 0 1 1 0 0 0 4 4
Bud Neck 0 0 0 0 0 1 0 – 1 3 0 0 1 0 0 0 0 0 0 0 0 1 2 3
Bud Site 0 0 0 1 0 8 1 – 8 10 5 10 0 0 0 1 0 1 – – – – – –
Cell Periphery 1 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0
Cytoplasm 3 1 3 12 20 16 24 – 14 12 14 4 18 59 114 22 20 20 0 0 0 0 2 2
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 2 4 4 8 0 0 0 0 1 0
Endosome 2 2 0 6 8 2 9 – 2 1 1 0 1 2 11 9 8 12 7 1 1 2 11 6
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 1 1 1 0 1 1 1 0
Mitochondria 27 23 4 21 16 156 31 – 106 100 79 48 13 11 2 33 19 23 1 1 1 2 3 7
Nucleus 157 79 78 259 397 270 487 – 273 349 189 141 458 571 624 302 237 222 148 89 78 166 422 377
Nuclear Periphery 1 0 0 0 3 1 6 – 3 4 3 0 0 2 2 1 1 3 2 0 0 0 1 3
Nucleolus 12 3 0 3 12 8 20 – 26 40 34 31 17 9 23 5 4 5 23 12 17 52 120 126
Peroxisomes 0 0 0 0 0 0 1 – 0 0 0 0 0 2 3 0 1 3 0 0 0 0 0 1
SpindlePole 3 1 6 16 12 12 23 – 4 7 5 2 11 15 8 17 18 14 4 3 7 8 31 32
Vac/Vac Membrane 3 6 0 2 9 2 6 – 3 1 0 1 1 0 6 11 12 5 10 10 12 16 45 44
Unique Cell Count 179 100 85 290 447 347 559 348 428 263 172 495 647 771 352 282 278 205 121 125 256 651 618
Labelled Cell Count 209 115 91 321 478 477 612 444 533 331 237 520 673 795 409 326 318 205 121 125 256 651 618


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 4.8 5.0 5.4 6.1 4.4 5.3 – 4.9 5.0 5.3 4.9 5.5 5.2 5.9 6.2 6.6 6.4 4.8 5.2 5.3
Std Deviation (1e-4) 0.7 0.7 1.0 1.3 1.6 1.5 1.2 – 1.5 1.3 1.7 1.1 0.9 1.1 1.4 1.1 1.1 1.4 1.1 1.1 1.3
Intensity Change (Log2) – – – 0.11 0.3 -0.19 0.1 – -0.01 0.01 0.1 -0.04 0.15 0.07 0.23 0.31 0.4 0.35 -0.05 0.05 0.08


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –