Standard name
Human Ortholog
Description Translation termination factor eRF3; has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that modifies cellular fitness, alters translational fidelity by affecting reading frame selection, and results in a nonsense suppressor phenotype; many stress-response genes are repressed in the presence of [PSI(+)]
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0.05 0.07 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0.09 0.05 0 0.07 0.11 0.08 0.06 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 1.0 0.99 0.97 0.96 0.96 0.87 0.91 0.86 0.87 0.84 0.73 0.88 0.96 0.96 0.99 – – – 0.98 0.94 0.92 0.91 0.94 0.88
Endoplasmic Reticulum 0 0 0 0 0.05 0.09 0.08 0.09 0.07 0.09 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 – – – 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 – – – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 1 1 6 7 2 0 0 0 – – – 0 0 0 0 0 1
Bud 0 0 0 0 0 0 1 0 2 0 0 0 0 0 0 – – – 0 2 1 2 2 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 2 4 2 0 9 7 5 4 14 8 5 3 5 5 – – – 0 0 0 0 0 0
Cytoplasm 62 206 131 54 76 87 141 117 47 108 69 72 135 143 144 – – – 83 197 123 110 148 147
Endoplasmic Reticulum 0 2 3 1 4 9 13 12 4 12 4 2 4 5 1 – – – 0 2 3 2 1 3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 1 1 0 0 1 0 0 0 4 2 1 0 1 0 – – – 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 1 0 1 6 2 0 0 0 – – – 0 0 0 0 0 2
Nucleus 0 0 0 0 0 0 0 0 0 0 2 1 1 0 0 – – – 0 1 0 1 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 1 0 1 0 0 0 1 0 0 6 0 1 1 1 – – – 0 2 2 2 1 1
Unique Cell Count 62 208 135 56 79 100 155 136 54 129 95 82 140 149 146 85 211 134 121 158 168
Labelled Cell Count 62 212 139 58 80 107 162 137 58 145 106 85 144 155 151 85 211 134 121 158 168


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 52.3 77.4 56.7 50.1 46.2 41.7 40.4 37.8 36.0 36.0 31.0 31.7 54.2 53.1 52.5 – – – 47.2 51.6 50.7
Std Deviation (1e-4) 11.0 15.8 11.0 9.7 11.3 11.0 9.3 10.4 10.2 9.0 9.8 8.7 17.5 18.8 20.8 – – – 15.9 15.6 20.8
Intensity Change (Log2) – – – -0.18 -0.3 -0.44 -0.49 -0.58 -0.66 -0.65 -0.87 -0.84 -0.06 -0.1 -0.11 – – – -0.26 -0.14 -0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cell Periphery 0 0 2.0 0.7 0 0 2.5 0 0 0 0 0 – – –
Cytoplasm -0.2 -0.3 -2.9 -2.1 -3.3 -2.7 -3.7 -5.4 -2.7 -0.3 -0.5 0.9 – – –
Endoplasmic Reticulum 0 0 2.3 2.3 2.4 0 2.5 0 0 0 0 0 – – –
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 – – –