Standard name | |
---|---|
Human Ortholog | |
Description | Alternate beta-subunit of the Snf1p kinase complex; may confer substrate specificity; vacuolar protein containing KIS (Kinase-Interacting Sequence) and ASC (Association with Snf1 kinase Complex) domains involved in protein interactions |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 2 | 1 |
Bud | 1 | 0 | 0 | 1 | 1 | 0 | 2 | 1 | 3 | 12 | 16 | 4 | 4 | 0 | 4 | 0 | 0 | 0 | 0 | 1 | 4 | 2 | 2 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 5 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 4 | 2 | 4 | 4 | 3 | 2 | 6 | 3 | 5 | 7 | 1 | 1 | 2 | 3 | 7 | 7 | 17 | 14 | 0 | 0 | 0 | 1 | 0 | 0 |
Cytoplasm | 169 | 135 | 241 | 251 | 317 | 259 | 378 | 307 | 301 | 300 | 196 | 297 | 230 | 321 | 412 | 265 | 237 | 206 | 159 | 138 | 204 | 140 | 242 | 210 |
Endoplasmic Reticulum | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 1 | 1 | 12 | 12 | 8 | 2 | 0 | 2 | 9 | 11 | 9 |
Endosome | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 2 | 2 | 2 | 3 | 2 | 0 | 0 | 1 | 0 | 1 | 1 | 3 | 0 | 2 | 0 | 1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3 | 11 | 0 | 0 | 2 | 16 | 43 | 16 | 79 | 96 | 94 | 49 | 2 | 2 | 1 | 6 | 1 | 3 | 2 | 1 | 5 | 6 | 9 | 7 |
Nucleus | 1 | 0 | 1 | 1 | 0 | 3 | 3 | 8 | 4 | 2 | 2 | 1 | 0 | 0 | 1 | 4 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 1 | 3 | 2 | 2 | 1 | 3 | 1 | 3 | 1 | 2 | 1 | 0 | 13 | 15 | 5 | 2 | 2 | 4 | 16 | 15 | 15 |
Unique Cell Count | 173 | 141 | 242 | 255 | 320 | 267 | 398 | 320 | 334 | 350 | 254 | 328 | 240 | 322 | 416 | 278 | 250 | 215 | 177 | 153 | 229 | 184 | 291 | 261 |
Labelled Cell Count | 179 | 149 | 247 | 260 | 327 | 283 | 436 | 339 | 399 | 422 | 318 | 358 | 245 | 328 | 428 | 308 | 287 | 239 | 177 | 153 | 229 | 184 | 291 | 261 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.8 | 4.4 | 6.1 | 5.6 | 5.9 | 4.9 | 5.0 | 5.5 | 4.4 | 4.4 | 4.3 | 4.8 | 5.9 | 6.0 | 6.1 | 8.8 | 8.8 | 8.6 | 5.5 | 5.5 | 6.0 |
Std Deviation (1e-4) | 1.0 | 0.8 | 1.5 | 1.0 | 1.2 | 1.5 | 1.5 | 1.6 | 1.2 | 1.2 | 1.5 | 1.2 | 1.2 | 0.9 | 1.4 | 1.7 | 1.6 | 1.4 | 1.1 | 1.0 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.12 | -0.05 | -0.3 | -0.28 | -0.16 | -0.48 | -0.48 | -0.5 | -0.34 | -0.05 | -0.02 | -0.0 | 0.53 | 0.53 | 0.49 | -0.14 | -0.16 | -0.03 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 3.4 | 3.9 | – |