ORF | |
---|---|
Human Ortholog | |
Description | Protein of unknown function; non-essential gene; expression induced upon calcium shortage; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 2 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 |
Bud | 0 | 1 | 0 | 0 | 0 | 2 | 0 | – | 1 | 2 | 1 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 2 | 0 | 3 | 1 | 3 | – | 2 | 3 | 3 | 4 | 1 | 0 | 1 | 3 | 5 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 421 | 383 | 340 | 243 | 220 | 237 | 257 | – | 230 | 182 | 157 | 163 | 309 | 449 | 489 | 209 | 174 | 160 | 450 | 330 | 328 | 213 | 505 | 415 |
Endoplasmic Reticulum | 1 | 5 | 2 | 0 | 3 | 1 | 1 | – | 4 | 2 | 1 | 4 | 5 | 3 | 1 | 5 | 7 | 2 | 0 | 1 | 2 | 0 | 4 | 3 |
Endosome | 1 | 0 | 0 | 0 | 4 | 0 | 12 | – | 3 | 5 | 3 | 9 | 0 | 0 | 0 | 0 | 0 | 0 | 9 | 2 | 0 | 0 | 2 | 2 |
Golgi | 5 | 1 | 0 | 0 | 3 | 0 | 1 | – | 2 | 5 | 8 | 3 | 0 | 0 | 0 | 1 | 0 | 1 | 6 | 2 | 0 | 2 | 0 | 1 |
Mitochondria | 4 | 1 | 1 | 2 | 2 | 45 | 15 | – | 38 | 32 | 41 | 65 | 0 | 0 | 0 | 0 | 1 | 0 | 4 | 2 | 1 | 0 | 0 | 1 |
Nucleus | 8 | 12 | 9 | 14 | 34 | 53 | 99 | – | 88 | 107 | 125 | 133 | 3 | 5 | 4 | 8 | 5 | 4 | 42 | 41 | 8 | 4 | 2 | 6 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 0 |
Nucleolus | 3 | 0 | 0 | 0 | 0 | 0 | 0 | – | 2 | 0 | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 62 | 16 | 31 | 133 | 197 | 162 | 431 | – | 212 | 231 | 220 | 247 | 2 | 12 | 13 | 74 | 45 | 47 | 12 | 17 | 1 | 4 | 7 | 9 |
Unique Cell Count | 475 | 401 | 356 | 334 | 381 | 451 | 697 | 499 | 509 | 491 | 560 | 314 | 457 | 499 | 248 | 202 | 187 | 538 | 404 | 349 | 229 | 532 | 448 | |
Labelled Cell Count | 507 | 420 | 385 | 392 | 466 | 503 | 819 | 584 | 569 | 560 | 644 | 320 | 469 | 508 | 300 | 237 | 215 | 538 | 404 | 349 | 229 | 532 | 448 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 10.8 | 10.2 | 9.8 | 9.8 | 10.6 | 7.6 | 10.0 | – | 9.8 | 9.9 | 10.0 | 9.6 | 12.1 | 11.3 | 12.2 | 16.2 | 17.4 | 16.5 | 10.0 | 10.7 | 11.4 |
Std Deviation (1e-4) | 1.6 | 1.4 | 1.2 | 1.1 | 1.7 | 1.8 | 1.6 | – | 1.9 | 1.8 | 1.9 | 2.0 | 1.7 | 1.6 | 1.8 | 3.6 | 4.0 | 3.4 | 1.7 | 1.6 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.0 | 0.11 | -0.36 | 0.03 | – | -0.01 | 0.02 | 0.02 | -0.04 | 0.3 | 0.2 | 0.31 | 0.72 | 0.83 | 0.75 | 0.02 | 0.13 | 0.21 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -8.3 | -12.0 | -13.4 | -18.2 | – | -15.1 | -17.7 | -18.5 | -19.7 | 2.2 | 2.3 | 2.1 | -4.7 | -3.9 | -4.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 5.9 | 2.3 | – | 5.1 | 4.5 | 5.3 | 6.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 1.2 | 3.7 | 4.9 | 5.9 | – | 6.9 | 7.9 | 9.0 | 8.7 | -1.5 | -1.6 | -2.0 | 0.5 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 9.6 | 12.6 | 9.0 | 16.4 | – | 10.8 | 11.6 | 11.4 | 11.4 | -4.8 | -3.8 | -4.0 | 6.7 | 4.5 | 5.2 |