ORF
Human Ortholog
Description Protein of unknown function; non-essential gene; expression induced upon calcium shortage; protein abundance increases in response to DNA replication stress
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.89 0.96 0.96 0.73 0.58 0.53 0.37 – 0.46 0.36 0.32 0.29 0.98 0.98 0.98 0.84 0.86 0.86 0.84 0.82 0.94 0.93 0.95 0.93
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.1 0 – 0.08 0.06 0.08 0.12 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0.09 0.12 0.14 – 0.18 0.21 0.25 0.24 0 0 0 0 0 0 0.08 0.1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.13 0 0.09 0.4 0.52 0.36 0.62 – 0.42 0.45 0.45 0.44 0 0 0 0.3 0.22 0.25 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 – 2 0 0 3 0 0 0 0 0 0 0 1 0 0 2 0
Bud 0 1 0 0 0 2 0 – 1 2 1 7 0 0 0 0 0 0 1 1 0 0 0 2
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 2 0 3 1 3 – 2 3 3 4 1 0 1 3 5 1 0 0 0 0 0 0
Cytoplasm 421 383 340 243 220 237 257 – 230 182 157 163 309 449 489 209 174 160 450 330 328 213 505 415
Endoplasmic Reticulum 1 5 2 0 3 1 1 – 4 2 1 4 5 3 1 5 7 2 0 1 2 0 4 3
Endosome 1 0 0 0 4 0 12 – 3 5 3 9 0 0 0 0 0 0 9 2 0 0 2 2
Golgi 5 1 0 0 3 0 1 – 2 5 8 3 0 0 0 1 0 1 6 2 0 2 0 1
Mitochondria 4 1 1 2 2 45 15 – 38 32 41 65 0 0 0 0 1 0 4 2 1 0 0 1
Nucleus 8 12 9 14 34 53 99 – 88 107 125 133 3 5 4 8 5 4 42 41 8 4 2 6
Nuclear Periphery 1 0 0 0 0 1 0 – 0 0 0 1 0 0 0 0 0 0 1 0 1 0 2 0
Nucleolus 3 0 0 0 0 0 0 – 2 0 1 4 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Vac/Vac Membrane 62 16 31 133 197 162 431 – 212 231 220 247 2 12 13 74 45 47 12 17 1 4 7 9
Unique Cell Count 475 401 356 334 381 451 697 499 509 491 560 314 457 499 248 202 187 538 404 349 229 532 448
Labelled Cell Count 507 420 385 392 466 503 819 584 569 560 644 320 469 508 300 237 215 538 404 349 229 532 448


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.8 10.2 9.8 9.8 10.6 7.6 10.0 – 9.8 9.9 10.0 9.6 12.1 11.3 12.2 16.2 17.4 16.5 10.0 10.7 11.4
Std Deviation (1e-4) 1.6 1.4 1.2 1.1 1.7 1.8 1.6 – 1.9 1.8 1.9 2.0 1.7 1.6 1.8 3.6 4.0 3.4 1.7 1.6 1.9
Intensity Change (Log2) – – – -0.0 0.11 -0.36 0.03 – -0.01 0.02 0.02 -0.04 0.3 0.2 0.31 0.72 0.83 0.75 0.02 0.13 0.21

WT3RAP60RAP140RAP220RAP300RAP460RAP540RAP620RAP70005101520WT3HU80HU120HU16005101520WT3rpd3Δ_1rpd3Δ_2rpd3Δ_305101520WT1AF100AF140AF18005101520
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm -8.3 -12.0 -13.4 -18.2 – -15.1 -17.7 -18.5 -19.7 2.2 2.3 2.1 -4.7 -3.9 -4.1
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 5.9 2.3 – 5.1 4.5 5.3 6.5 0 0 0 0 0 0
Nucleus 1.2 3.7 4.9 5.9 – 6.9 7.9 9.0 8.7 -1.5 -1.6 -2.0 0.5 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 9.6 12.6 9.0 16.4 – 10.8 11.6 11.4 11.4 -4.8 -3.8 -4.0 6.7 4.5 5.2