Standard name | |
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Human Ortholog | |
Description | ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 5 | 1 | 5 | 4 | 8 | 11 | 8 | 5 | 7 | 4 | 6 | 25 | 27 | 32 | 9 | 9 | 0 | 0 | 0 | 1 | 0 | 1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 4 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 7 | 3 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 1 | 0 | 2 | 0 | 3 | 4 | 0 | 2 | 9 | 7 | 9 | 1 | 0 | 0 | 2 | 2 | 2 | 0 | 0 | 0 | 1 | 0 | 1 |
Nucleus | 42 | 58 | 146 | 65 | 99 | 88 | 200 | 142 | 124 | 120 | 110 | 106 | 141 | 174 | 176 | 168 | 95 | 98 | 53 | 77 | 122 | 88 | 145 | 158 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1 | 7 | 7 | 8 | 3 | 9 | 16 | 4 | 15 | 18 | 23 | 19 | 2 | 4 | 7 | 19 | 17 | 12 | 3 | 1 | 28 | 1 | 14 | 17 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 1 | 3 | 3 | 1 | 4 | 18 | 24 | 28 | 19 | 13 | 13 | 11 | 4 | 5 | 10 | 29 | 26 | 24 | 0 | 4 | 9 | 8 | 10 | 32 |
Unique Cell Count | 43 | 60 | 155 | 70 | 110 | 118 | 241 | 179 | 162 | 156 | 152 | 144 | 151 | 206 | 219 | 247 | 144 | 144 | 59 | 85 | 164 | 102 | 173 | 218 |
Labelled Cell Count | 44 | 69 | 161 | 77 | 111 | 123 | 257 | 187 | 170 | 169 | 162 | 151 | 154 | 208 | 226 | 263 | 156 | 156 | 59 | 85 | 164 | 102 | 173 | 218 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 30.0 | 39.6 | 34.1 | 27.2 | 33.9 | 27.1 | 28.6 | 28.9 | 26.5 | 26.2 | 23.6 | 22.9 | 32.2 | 33.6 | 31.5 | 44.0 | 41.8 | 44.3 | 36.7 | 32.4 | 35.4 |
Std Deviation (1e-4) | 6.7 | 9.9 | 10.0 | 7.1 | 13.8 | 6.4 | 7.2 | 8.2 | 7.7 | 7.8 | 7.6 | 6.9 | 9.8 | 13.4 | 12.4 | 17.4 | 14.7 | 18.3 | 13.8 | 10.4 | 11.9 |
Intensity Change (Log2) | – | – | – | -0.33 | -0.01 | -0.33 | -0.26 | -0.24 | -0.36 | -0.38 | -0.53 | -0.58 | -0.09 | -0.02 | -0.11 | 0.37 | 0.29 | 0.37 | 0.11 | -0.07 | 0.05 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0.1 | 1.2 | 0.8 | 0 | 0.6 | 0 | 0.4 | 3.0 | 3.1 | 3.3 | 1.2 | 1.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -0.4 | -1.3 | -4.6 | -3.3 | -3.9 | -4.4 | -4.3 | -5.1 | -4.9 | -0.3 | -2.9 | -3.8 | -6.2 | -6.2 | -5.8 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 1.1 | 0.9 | -1.2 | 1.7 | 2.3 | 3.1 | 2.7 | 0 | 0 | -0.7 | 1.3 | 2.3 | 1.4 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 4.1 | 3.1 | 4.3 | 3.4 | 2.6 | 2.6 | 2.3 | 0 | 0 | 1.4 | 3.5 | 4.7 | 4.4 |