Standard name
Human Ortholog
Description Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.13 0.18 0.08 0.08 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.21 0.44 0.47 0.38 0.55 0.51 0.54 0.69 0.65 0.64 0.76 0.09 0 0.14 0 0 0.07 0 0 0 0 0 0
Nucleus 0.86 0.89 0.86 0.76 0.68 0.75 0.72 0.72 0.69 0.72 0.66 0.66 0.85 0.79 0.69 0.81 0.79 0.76 0.75 0.87 0.78 0.77 0.76 0.52
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.11 0 0.05 0.13 0.18 0.13 0.13 0.13 0.13 0.13 0.1 0.09 0 0.06 0.1 0.05 0.08 0.06 0.07 0 0 0 0 0.06
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.07 0 0 0.08 0.1 0.14 0.28
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 1 3 3 2 4 4 4 6 1 0 1 0 0 0 0 2 1 0 0 2 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0 0 0 0 1 1
Bud Site 0 0 1 5 7 14 25 13 10 18 16 15 0 2 1 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 8 5 5 9 14 7 22 16 11 12 7 3 15 36 60 37 22 35 0 2 2 2 1 1
Endoplasmic Reticulum 1 1 0 0 0 0 0 0 0 0 0 1 0 0 0 13 8 13 1 0 0 1 0 1
Endosome 4 1 0 0 8 1 8 2 2 0 1 0 0 2 1 7 4 11 3 0 1 2 4 3
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 5 1 4 1 1 0 0 1 1
Mitochondria 5 67 98 146 147 257 328 366 230 276 166 222 13 6 47 18 4 30 2 5 0 1 2 12
Nucleus 200 287 191 237 264 347 463 488 229 307 172 193 116 217 232 383 230 344 175 283 177 81 225 146
Nuclear Periphery 4 4 6 4 9 10 21 10 8 13 15 14 0 7 10 7 8 12 2 1 1 0 0 3
Nucleolus 25 12 12 40 71 61 81 90 43 55 27 26 6 17 32 25 22 28 17 9 8 2 10 17
Peroxisomes 0 1 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 2 0 0 0 0 0 0
SpindlePole 0 0 3 7 19 3 16 11 6 4 4 1 0 3 2 1 3 6 12 2 9 0 4 7
Vac/Vac Membrane 3 4 1 3 1 3 6 2 1 1 3 6 0 4 12 21 26 31 10 12 17 10 40 79
Unique Cell Count 232 324 222 310 388 464 646 681 334 426 259 291 137 273 335 470 292 453 232 324 227 106 296 285
Labelled Cell Count 251 382 317 452 543 706 972 1002 544 690 419 483 150 297 399 517 328 516 232 324 227 106 296 285


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.7 4.9 4.1 4.1 4.1 3.8 3.8 3.8 3.5 3.4 3.5 3.1 5.9 6.2 5.2 8.7 9.2 9.5 5.5 5.5 5.7
Std Deviation (1e-4) 1.3 1.2 0.6 1.2 0.9 0.6 0.9 0.9 1.0 0.8 1.2 1.0 1.9 2.0 1.5 2.2 2.3 2.6 1.6 1.3 1.1
Intensity Change (Log2) – – – -0.01 0.02 -0.1 -0.11 -0.1 -0.24 -0.26 -0.22 -0.38 0.52 0.6 0.35 1.08 1.17 1.22 0.42 0.43 0.48


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 2.8 4.3 3.7