Standard name | |
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Human Ortholog | |
Description | Mitochondrial glycerol-3-phosphate dehydrogenase; expression is repressed by both glucose and cAMP and derepressed by non-fermentable carbon sources in a Snf1p, Rsf1p, Hap2/3/4/5 complex dependent manner |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 2 | 4 | 4 | 1 | 0 | 0 | – | 0 | 0 | 1 | 2 | 4 | 3 | 5 | 11 | 3 | 6 | 1 | 19 | 2 | 3 | 1 | 6 |
Bud | 11 | 10 | 1 | 4 | 9 | 10 | 8 | – | 7 | 8 | 7 | 12 | 0 | 3 | 0 | 16 | 5 | 10 | 3 | 3 | 0 | 1 | 1 | 1 |
Bud Neck | 2 | 0 | 2 | 1 | 0 | 0 | 0 | – | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 1 | 2 | 1 |
Bud Site | 2 | 1 | 0 | 0 | 0 | 0 | 4 | – | 3 | 2 | 7 | 5 | 0 | 0 | 0 | 1 | 2 | 8 | – | – | – | – | – | – |
Cell Periphery | 5 | 17 | 3 | 4 | 5 | 6 | 3 | – | 1 | 3 | 4 | 1 | 1 | 3 | 7 | 9 | 14 | 12 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 15 | 17 | 27 | 17 | 15 | 11 | 30 | – | 10 | 11 | 20 | 5 | 2 | 29 | 55 | 19 | 18 | 15 | 1 | 3 | 2 | 2 | 4 | 1 |
Endoplasmic Reticulum | 4 | 10 | 6 | 2 | 6 | 7 | 3 | – | 4 | 1 | 3 | 6 | 6 | 4 | 10 | 6 | 5 | 3 | 2 | 4 | 0 | 1 | 1 | 2 |
Endosome | 18 | 44 | 15 | 10 | 30 | 15 | 15 | – | 1 | 10 | 0 | 0 | 7 | 15 | 18 | 20 | 26 | 22 | 31 | 40 | 24 | 13 | 10 | 18 |
Golgi | 4 | 22 | 3 | 7 | 16 | 4 | 7 | – | 0 | 2 | 0 | 0 | 17 | 16 | 23 | 56 | 73 | 59 | 16 | 46 | 3 | 3 | 9 | 8 |
Mitochondria | 330 | 322 | 40 | 117 | 156 | 234 | 271 | – | 229 | 225 | 296 | 276 | 23 | 52 | 23 | 20 | 26 | 22 | 274 | 352 | 169 | 122 | 156 | 184 |
Nucleus | 3 | 0 | 2 | 2 | 2 | 8 | 10 | – | 5 | 10 | 3 | 8 | 0 | 1 | 2 | 4 | 6 | 2 | 1 | 1 | 0 | 0 | 1 | 2 |
Nuclear Periphery | 3 | 6 | 1 | 1 | 2 | 5 | 7 | – | 3 | 4 | 12 | 8 | 1 | 0 | 0 | 0 | 4 | 0 | 3 | 3 | 0 | 0 | 0 | 3 |
Nucleolus | 3 | 1 | 0 | 0 | 3 | 4 | 5 | – | 8 | 2 | 4 | 4 | 0 | 1 | 0 | 14 | 8 | 12 | 1 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 4 | 28 | 5 | 14 | 3 | 6 | 4 | – | 0 | 1 | 2 | 1 | 1 | 11 | 3 | 40 | 36 | 55 | 10 | 16 | 11 | 8 | 3 | 9 |
SpindlePole | 9 | 11 | 15 | 12 | 7 | 7 | 14 | – | 8 | 13 | 8 | 9 | 2 | 4 | 3 | 48 | 42 | 51 | 4 | 3 | 1 | 2 | 3 | 4 |
Vac/Vac Membrane | 46 | 84 | 25 | 9 | 43 | 29 | 28 | – | 12 | 19 | 22 | 13 | 12 | 19 | 19 | 36 | 31 | 52 | 25 | 24 | 10 | 8 | 15 | 15 |
Unique Cell Count | 366 | 438 | 129 | 160 | 243 | 289 | 334 | 252 | 260 | 324 | 299 | 58 | 126 | 136 | 235 | 241 | 246 | 385 | 530 | 233 | 177 | 218 | 266 | |
Labelled Cell Count | 459 | 575 | 149 | 204 | 298 | 346 | 409 | 291 | 312 | 389 | 351 | 76 | 161 | 168 | 300 | 299 | 329 | 385 | 530 | 233 | 177 | 218 | 266 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.9 | 6.0 | 5.9 | 6.2 | 6.8 | 5.9 | 6.1 | – | 5.1 | 5.0 | 4.5 | 4.6 | 6.9 | 6.9 | 6.5 | 10.8 | 11.4 | 11.5 | 5.2 | 5.9 | 6.0 |
Std Deviation (1e-4) | 0.7 | 1.1 | 1.1 | 1.7 | 1.4 | 1.4 | 1.7 | – | 1.5 | 1.2 | 1.4 | 0.9 | 0.9 | 1.2 | 1.1 | 2.6 | 3.0 | 3.0 | 1.1 | 1.3 | 1.4 |
Intensity Change (Log2) | – | – | – | 0.09 | 0.22 | 0.0 | 0.05 | – | -0.2 | -0.24 | -0.37 | -0.34 | 0.23 | 0.24 | 0.15 | 0.88 | 0.96 | 0.97 | -0.16 | 0.02 | 0.04 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.7 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.6 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.5 | 1.2 |
Cytoplasm | -2.4 | -4.3 | -5.6 | -3.5 | – | -5.3 | -5.2 | -4.6 | -7.0 | -3.1 | 0.4 | 3.4 | -3.5 | -3.8 | -4.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0.9 | 0 | 0 | 0 |
Endosome | -1.6 | 0.2 | -2.4 | -2.8 | – | -5.2 | -2.9 | 0 | 0 | 0.1 | 0.1 | 0.4 | -1.0 | -0.2 | -0.8 |
Golgi | 0 | 1.8 | 0 | 0 | – | 0 | 0 | 0 | 0 | 5.5 | 3.2 | 4.0 | 5.3 | 6.3 | 5.4 |
Mitochondria | 7.1 | 6.1 | 9.9 | 10.3 | – | 12.1 | 11.1 | 0 | 0 | 1.2 | 1.7 | -2.7 | -5.5 | -4.8 | -5.5 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 1.7 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1.6 | 0 | 3.6 | 3.2 | 4.6 |
SpindlePole | -1.2 | -3.4 | -3.9 | -3.0 | – | -3.3 | -2.4 | 0 | 0 | -1.8 | -2.6 | -3.0 | 2.1 | 1.5 | 2.2 |
Vacuole | -3.6 | -0.4 | -2.6 | -3.3 | – | -4.6 | -3.5 | -4.0 | -5.0 | 0.2 | -0.9 | -1.2 | -1.0 | -1.7 | 0.4 |