Standard name | |
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Human Ortholog | |
Description | Subunit of DNA polymerase delta (polymerase III); essential for cell viability; involved in DNA replication and DNA repair; forms a complex with Rev3p, Rev7p and Pol32p; relocalizes to the cytosol in response to hypoxia |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 |
Bud | 0 | 0 | 1 | 0 | 1 | 4 | 6 | 17 | – | 7 | 6 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 1 | 2 | 2 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 1 | 1 | 0 | 13 | 22 | 15 | – | 22 | 20 | 27 | 0 | 0 | 0 | 1 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 11 | 9 | 15 | 16 | 21 | 27 | 35 | 36 | – | 35 | 23 | 25 | 32 | 43 | 52 | 21 | 10 | 14 | 1 | 2 | 1 | 1 | 3 | 2 |
Endoplasmic Reticulum | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 2 | 2 | 8 | 10 | 8 | 0 | 0 | 1 | 0 | 3 | 2 |
Endosome | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 3 | 2 | 0 | 0 | 2 | 3 | 2 | 5 | 0 | 1 | 6 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2 | 1 | 0 | 2 | 0 | 0 | 3 | 0 | 2 | 0 | 0 | 1 |
Mitochondria | 10 | 10 | 134 | 73 | 140 | 298 | 439 | 377 | – | 401 | 413 | 445 | 2 | 4 | 0 | 23 | 27 | 10 | 4 | 1 | 2 | 0 | 2 | 6 |
Nucleus | 230 | 210 | 381 | 278 | 274 | 410 | 467 | 486 | – | 434 | 417 | 430 | 230 | 280 | 261 | 417 | 385 | 351 | 225 | 204 | 380 | 116 | 123 | 134 |
Nuclear Periphery | 0 | 1 | 0 | 1 | 3 | 1 | 14 | 6 | – | 5 | 3 | 6 | 0 | 0 | 0 | 7 | 4 | 5 | 0 | 0 | 2 | 0 | 0 | 0 |
Nucleolus | 1 | 3 | 5 | 3 | 8 | 17 | 27 | 58 | – | 27 | 26 | 26 | 1 | 0 | 0 | 6 | 4 | 2 | 4 | 5 | 11 | 1 | 2 | 4 |
Peroxisomes | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 1 | 2 | 0 | 4 | 3 | 5 | – | 3 | 3 | 0 | 1 | 4 | 3 | 4 | 1 | 2 | 2 | 1 | 6 | 0 | 1 | 3 |
Vac/Vac Membrane | 9 | 11 | 2 | 1 | 7 | 8 | 12 | 8 | – | 9 | 14 | 10 | 2 | 2 | 4 | 22 | 16 | 14 | 9 | 10 | 29 | 19 | 42 | 44 |
Unique Cell Count | 250 | 227 | 438 | 306 | 343 | 536 | 683 | 686 | 631 | 582 | 607 | 264 | 331 | 321 | 462 | 413 | 382 | 263 | 236 | 453 | 145 | 190 | 215 | |
Labelled Cell Count | 264 | 244 | 541 | 376 | 454 | 782 | 1025 | 1008 | 943 | 925 | 973 | 272 | 340 | 326 | 511 | 457 | 408 | 263 | 236 | 453 | 145 | 190 | 215 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.9 | 5.8 | 4.1 | 4.3 | 4.0 | 3.9 | 3.6 | 3.6 | – | 3.3 | 3.3 | 3.3 | 6.1 | 6.2 | 6.2 | 7.0 | 7.0 | 7.4 | 6.5 | 7.0 | 6.8 |
Std Deviation (1e-4) | 0.9 | 0.8 | 0.8 | 1.3 | 1.2 | 1.2 | 1.6 | 1.4 | – | 0.9 | 1.0 | 0.8 | 1.3 | 1.3 | 1.4 | 1.4 | 1.3 | 1.5 | 1.4 | 1.7 | 1.6 |
Intensity Change (Log2) | – | – | – | 0.04 | -0.06 | -0.1 | -0.22 | -0.21 | – | -0.33 | -0.33 | -0.34 | 0.57 | 0.57 | 0.57 | 0.77 | 0.75 | 0.83 | 0.66 | 0.75 | 0.72 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.2 | 1.8 | 1.2 | 1.3 | 1.4 | – | 1.6 | 0.4 | 0.6 | 4.5 | 5.0 | 6.1 | 0.9 | -0.9 | 0.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | -9.7 | -10.5 | -10.9 | -10.1 | -9.0 | -10.5 |
Nucleus | 1.6 | -2.7 | -4.2 | -7.1 | -6.3 | – | -6.9 | -5.9 | -6.2 | 0.1 | -0.9 | -2.1 | 1.5 | 3.0 | 2.3 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0.2 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 1.9 | 0 | – | 0 | 2.5 | 1.8 | 0 | 0 | 0 | 4.0 | 3.5 | 3.3 |