Standard name
Human Ortholog
Description Cell wall mannoprotein that localizes to birth scars of daughter cells; linked to a beta-1,3- and beta-1,6-glucan heteropolymer through a phosphodiester bond; required for propionic acid resistance
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0.05 0 – 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0.09 0 – 0 0 0.05 0.07 0 0 0 0 0 0 0.07 0.1 0 0 0.08 0.07
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0.06 0 0.07 – 0.12 0.13 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.66 0.73 0.82 0.88 0.61 0.57 0.52 – 0.61 0.52 0.56 0.6 0.84 0.87 0.88 0.78 0.7 0.83 0.14 0.2 0.36 0.43 0.27 0.21
Endoplasmic Reticulum 0.14 0.11 0.1 0.1 0.17 0.17 0.22 – 0.19 0.24 0.17 0.1 0.11 0.07 0.05 0.07 0.09 0 0.38 0.27 0.18 0.13 0.16 0.1
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.26 0.05 – 0.14 0.15 0.19 0.25 0 0 0 0 0 0 0 0 0 0.06 0 0
Nucleus 0 0 0 0 0.06 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.25 0.21 0.11 0 0.17 0.08 0.2 – 0.06 0.07 0.11 0.06 0 0.05 0.07 0.22 0.32 0.18 0.3 0.3 0.33 0.21 0.35 0.43
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 3 0 0 0 4 7 – 6 11 5 3 1 0 2 0 0 0 3 9 0 4 1 1
Bud 0 6 0 0 0 8 5 – 1 6 6 8 0 0 1 0 0 0 22 62 3 7 22 20
Bud Neck 0 0 1 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 4 0 1 1 4
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 4 1 0 1 1 2 12 – 17 21 7 0 5 1 5 0 0 0 0 1 0 0 0 0
Cytoplasm 201 415 79 44 11 50 96 – 85 85 70 70 174 168 260 193 132 121 48 126 38 98 79 61
Endoplasmic Reticulum 44 60 10 5 3 15 41 – 27 40 22 12 23 14 16 16 16 5 128 167 18 28 48 28
Endosome 10 13 2 0 0 0 1 – 0 1 0 3 5 7 8 2 7 4 10 19 1 8 9 20
Golgi 0 0 0 0 0 0 1 – 0 1 1 0 1 0 0 1 0 1 3 1 0 3 2 6
Mitochondria 4 0 0 1 0 23 9 – 19 25 24 29 0 0 1 10 2 5 8 10 4 14 7 5
Nucleus 4 2 0 1 1 0 2 – 2 1 1 3 0 0 0 7 6 0 0 2 0 1 1 1
Nuclear Periphery 0 0 1 0 0 0 0 – 2 1 2 1 1 0 0 0 0 0 5 6 0 3 3 1
Nucleolus 1 0 0 0 0 0 0 – 0 1 0 1 0 0 0 1 1 0 0 0 0 0 0 3
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 1 1 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 0 0 0 0 1 – 0 0 0 1 1 1 1 0 1 0 1 11 0 3 7 9
Vac/Vac Membrane 75 122 11 1 3 7 36 – 9 12 14 7 9 10 20 54 61 26 101 186 35 47 105 127
Unique Cell Count 305 568 96 50 18 88 183 140 164 126 117 207 193 296 246 188 146 341 617 105 228 297 298
Labelled Cell Count 345 623 104 53 19 109 211 168 205 152 139 221 201 315 284 226 162 341 617 105 228 297 298


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.8 8.6 6.2 5.0 9.4 5.6 9.1 – 8.6 9.3 9.1 7.8 8.2 6.3 7.1 6.9 9.0 6.1 6.2 6.8 7.5
Std Deviation (1e-4) 3.6 4.4 2.6 0.9 3.1 2.1 5.0 – 3.5 4.4 4.2 4.0 4.8 3.1 3.6 2.7 18.5 1.9 5.7 5.1 6.6
Intensity Change (Log2) – – – -0.31 0.6 -0.14 0.56 – 0.48 0.58 0.55 0.34 0.4 0.02 0.19 0.16 0.54 -0.01 -0.01 0.14 0.28


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 3.5 3.7 0 0 0 0 0 0 0 0
Cytoplasm 0.9 -2.0 -3.8 -4.9 – -3.5 -4.9 -4.2 -3.6 0.4 1.1 1.4 -0.8 -2.2 0.1
Endoplasmic Reticulum -0.1 0 1.3 2.5 – 1.8 2.8 1.5 0 0.2 -0.9 -1.7 -1.2 -0.5 -2.2
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 5.4 0 – 3.8 4.0 4.5 5.2 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 -0.8 1.7 – -1.4 -1.1 -0.1 -1.4 -2.3 -1.9 -1.5 2.2 3.8 1.3