Standard name
Human Ortholog
Description Protein required for rDNA silencing and mitotic rDNA condensation; stimulates Cdc14p phosphatase activity and biphasic release to promote rDNA repeat segregation; required for condensin recruitment to the replication fork barrier site; TOF2 has a paralog, NET1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.1 0.12 0.13 0.11 0.15 0.14 0.09 0.12 0.15 0.13 0.06 0.05 0.06 0 0 0 0 0 0 0 0 0.05
Bud Site 0 0 0 0 0 0.08 0.05 0.06 0.06 0.09 0.1 0.07 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0 0 0 0 0 0 0 0.09 0.14 0.17 0.07 0.07 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0.12 0.06 0.07 0.08 0.06 0.1 0.08
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.1 0.18 0 0 0 0.09 0.09 0 0.15 0.12 0.18 0.09 0.05 0 0 0 0 0.05 0 0 0 0 0 0
Nucleus 0.25 0.28 0.25 0.14 0.1 0.1 0.1 0.12 0.11 0.08 0.07 0.09 0.1 0.06 0.1 0.09 0.11 0.12 0.06 0.1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.67 0.53 0.34 0.41 0.3 0.43 0.35 0.4 0.49 0.49 0.46 0.44 0.65 0.55 0.52 0.69 0.59 0.6 0.44 0.46 0.38 0.22 0.18 0.21
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0 0
SpindlePole 0.4 0.38 0.72 0.77 0.81 0.78 0.72 0.78 0.74 0.72 0.66 0.75 0.49 0.6 0.51 0.64 0.61 0.6 0.36 0.27 0.4 0.62 0.54 0.5
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1
Bud 0 1 1 5 8 3 9 6 8 10 12 13 0 1 1 0 0 0 0 3 0 1 2 4
Bud Neck 3 1 26 22 34 28 51 46 24 34 36 37 14 11 13 3 5 1 2 2 5 1 7 11
Bud Site 2 2 2 4 10 19 17 21 15 26 24 19 3 1 1 1 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 3 8 6 13 4 11 11 3 7 7 9 21 30 38 15 12 12 1 0 1 0 1 1
Endoplasmic Reticulum 1 0 1 0 0 0 0 0 0 0 0 0 0 1 0 4 1 2 0 0 0 0 1 1
Endosome 2 3 4 0 1 2 0 0 0 0 0 1 9 9 7 13 10 18 9 18 23 10 20 19
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 1 1 0 1 4
Mitochondria 13 40 7 8 1 22 29 11 41 34 44 25 13 3 3 5 3 7 1 3 1 0 2 4
Nucleus 31 64 64 26 27 25 32 40 30 22 17 25 24 13 24 20 18 19 8 26 10 4 6 4
Nuclear Periphery 1 2 1 0 0 0 1 0 0 1 1 0 1 1 0 1 1 0 0 2 2 0 0 0
Nucleolus 84 119 88 78 80 108 115 135 131 143 112 124 160 119 120 148 101 92 62 122 107 37 40 51
Peroxisomes 0 0 0 1 2 0 0 3 0 0 0 0 7 4 12 1 10 2 1 1 1 1 3 4
SpindlePole 50 86 186 147 218 196 239 265 201 208 159 214 122 131 117 137 104 92 50 71 113 107 118 123
Vac/Vac Membrane 0 1 1 0 2 0 0 0 0 0 0 0 0 0 0 6 5 8 1 2 5 3 5 5
Unique Cell Count 125 225 258 190 270 252 332 340 270 289 241 285 247 218 229 213 171 153 142 264 283 174 218 247
Labelled Cell Count 190 322 389 297 396 408 505 538 453 485 412 467 374 324 336 355 273 253 142 264 283 174 218 247


Nucleolus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 5.1 5.5 5.0 5.5 4.2 4.4 4.7 3.9 3.7 3.5 4.1 5.1 5.6 5.1 8.9 9.6 8.7 5.2 5.4 5.5
Std Deviation (1e-4) 0.8 0.8 1.0 1.3 1.4 0.7 1.0 1.1 0.8 0.7 0.8 1.0 1.1 1.5 1.4 1.9 2.6 2.3 1.6 1.6 1.6
Intensity Change (Log2) – – – -0.15 0.0 -0.38 -0.32 -0.22 -0.51 -0.59 -0.67 -0.43 -0.12 0.0 -0.11 0.69 0.79 0.65 -0.1 -0.03 -0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0.5 0.9 0 0 0 0 0 0 0 -1.8 -2.0 -1.8 -3.9 -2.8 -3.7
Bud Site 0 2.3 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 1.0 -1.1 0.1 0.1 -1.6 -0.5 -0.1 0 2.6 4.3 5.1 2.0 1.9 2.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 1.5 1.7 1.1 2.6 2.5 4.4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.9 0 0 0 0 0 0 0 0 1.5 0 0 -0.3 0 1.0
Nucleus -2.9 -4.5 -4.4 -5.0 -4.2 -4.1 -5.5 -5.4 -5.0 -4.5 -5.6 -4.1 -4.4 -3.7 -3.0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1.5 -1.1 0 0 0 0 0 0 0 6.9 4.5 4.1 7.6 5.1 5.1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 3.7 0 0 0
SpindlePole 1.3 2.3 0 0 0 0 0 0 0 -5.2 -2.8 -4.8 -1.8 -2.4 -2.5
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0