ORF | |
---|---|
Human Ortholog | |
Description | Protein of unknown function; similar to Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; YKR075C has a paralog, YOR062C, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 2 | 4 | 0 | 2 | 1 |
Bud | 0 | 0 | 0 | 1 | 4 | 2 | 0 | 2 | 9 | 2 | 6 | 4 | 0 | 0 | 0 | – | 0 | 0 | 9 | 6 | 1 | 0 | 4 | 3 |
Bud Neck | 0 | 0 | 4 | 1 | 11 | 6 | 10 | 12 | 2 | 9 | 4 | 2 | 0 | 2 | 1 | – | 1 | 2 | 1 | 0 | 1 | 0 | 2 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 6 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 2 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 3 | – | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 142 | 174 | 296 | 195 | 227 | 327 | 265 | 320 | 99 | 169 | 67 | 159 | 326 | 368 | 400 | – | 176 | 164 | 115 | 142 | 228 | 184 | 257 | 219 |
Endoplasmic Reticulum | 1 | 4 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | – | 13 | 15 | 0 | 0 | 1 | 1 | 0 | 1 |
Endosome | 1 | 2 | 2 | 1 | 0 | 1 | 2 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | – | 3 | 0 | 3 | 6 | 5 | 3 | 5 | 4 |
Golgi | 0 | 1 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 1 | 4 | 2 | 0 | 0 | 0 |
Mitochondria | 13 | 12 | 0 | 9 | 20 | 49 | 33 | 57 | 255 | 119 | 271 | 206 | 0 | 0 | 0 | – | 4 | 5 | 5 | 14 | 27 | 3 | 13 | 7 |
Nucleus | 138 | 20 | 2 | 14 | 4 | 28 | 34 | 25 | 148 | 83 | 254 | 81 | 5 | 0 | 0 | – | 27 | 17 | 185 | 40 | 3 | 8 | 2 | 1 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 1 | 4 | 9 | 12 | 0 | 6 | 7 | 12 | 1 | 0 | 0 | – | 0 | 1 | 23 | 10 | 4 | 4 | 7 | 4 |
Vac/Vac Membrane | 84 | 33 | 3 | 11 | 6 | 25 | 18 | 30 | 22 | 14 | 11 | 42 | 1 | 2 | 0 | – | 69 | 23 | 59 | 29 | 5 | 8 | 10 | 3 |
Unique Cell Count | 341 | 231 | 304 | 225 | 248 | 400 | 341 | 415 | 405 | 345 | 479 | 406 | 330 | 368 | 402 | 260 | 205 | 416 | 266 | 301 | 222 | 321 | 256 | |
Labelled Cell Count | 379 | 248 | 312 | 233 | 275 | 444 | 374 | 459 | 539 | 405 | 631 | 510 | 334 | 372 | 405 | 295 | 228 | 416 | 266 | 301 | 222 | 321 | 256 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.3 | 4.0 | 4.6 | 4.5 | 3.9 | 3.9 | 3.9 | 4.3 | 3.5 | 3.8 | 3.8 | 3.7 | 4.5 | 4.7 | 4.3 | 4.3 | 5.3 | 5.4 | 4.7 | 5.1 | 5.4 |
Std Deviation (1e-4) | 0.7 | 1.2 | 1.1 | 1.4 | 1.0 | 1.1 | 1.2 | 1.2 | 1.0 | 1.0 | 0.8 | 0.7 | 0.6 | 0.9 | 0.8 | 1.3 | 1.4 | 1.3 | 1.2 | 1.4 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.05 | -0.24 | -0.24 | -0.23 | -0.1 | -0.38 | -0.29 | -0.28 | -0.32 | -0.02 | 0.02 | -0.1 | -0.11 | 0.2 | 0.22 | 0.02 | 0.14 | 0.23 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | 9.1 | 5.1 |