Standard name | |
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Human Ortholog | |
Description | Protein that localizes to sites of polarized growth; required for selection and/or maintenance of polarized growth sites, may modulate signaling by the GTPases Cdc42p and Rho1p; contains LIM domains and has similarity to metazoan paxillin; relocalizes from bud neck to cytoplasm upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 65 | 38 | 60 | 29 | 36 | 35 | 27 | 15 | 19 | 17 | 6 | 9 | 10 | 6 | 1 | 16 | 16 | 3 | 32 | 12 | 15 | 3 | 20 | 13 |
Bud | 74 | 27 | 39 | 13 | 25 | 32 | 46 | 32 | 27 | 29 | 24 | 31 | 7 | 3 | 2 | 5 | 7 | 3 | 107 | 66 | 70 | 6 | 10 | 18 |
Bud Neck | 7 | 9 | 13 | 6 | 12 | 13 | 16 | 15 | 1 | 5 | 3 | 11 | 0 | 0 | 0 | 0 | 2 | 0 | 64 | 46 | 62 | 8 | 8 | 9 |
Bud Site | 38 | 21 | 44 | 26 | 12 | 28 | 14 | 6 | 10 | 7 | 3 | 4 | 11 | 2 | 1 | 9 | 7 | 1 | – | – | – | – | – | – |
Cell Periphery | 9 | 10 | 9 | 16 | 3 | 2 | 6 | 7 | 9 | 2 | 5 | 7 | 1 | 8 | 1 | 13 | 13 | 3 | 2 | 1 | 2 | 0 | 2 | 1 |
Cytoplasm | 153 | 174 | 212 | 222 | 272 | 317 | 430 | 431 | 216 | 212 | 219 | 288 | 87 | 190 | 228 | 53 | 63 | 9 | 116 | 142 | 169 | 23 | 80 | 94 |
Endoplasmic Reticulum | 2 | 8 | 6 | 2 | 3 | 3 | 2 | 0 | 2 | 5 | 2 | 4 | 1 | 0 | 0 | 2 | 3 | 0 | 2 | 1 | 1 | 1 | 8 | 5 |
Endosome | 22 | 7 | 1 | 1 | 1 | 3 | 3 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 5 | 4 | 2 | 1 | 1 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 0 | 0 | 0 |
Mitochondria | 14 | 11 | 2 | 6 | 7 | 62 | 83 | 66 | 130 | 114 | 121 | 104 | 3 | 1 | 4 | 5 | 3 | 4 | 3 | 3 | 17 | 2 | 5 | 9 |
Nucleus | 6 | 0 | 3 | 2 | 0 | 3 | 3 | 9 | 3 | 2 | 2 | 4 | 0 | 2 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 3 | 3 | 1 | 1 | 0 | 4 | 4 | 7 | 1 | 1 | 1 | 2 | 2 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 19 | 12 | 2 | 4 | 0 | 15 | 4 | 1 | 3 | 2 | 0 | 1 | 7 | 3 | 2 | 0 | 2 | 1 | 0 | 0 | 1 | 1 | 0 | 0 |
SpindlePole | 35 | 5 | 45 | 30 | 18 | 47 | 38 | 34 | 12 | 9 | 7 | 17 | 2 | 1 | 0 | 7 | 7 | 1 | 8 | 5 | 8 | 0 | 0 | 1 |
Vac/Vac Membrane | 4 | 3 | 6 | 4 | 3 | 4 | 3 | 23 | 2 | 8 | 5 | 2 | 0 | 0 | 0 | 3 | 10 | 0 | 4 | 8 | 7 | 1 | 4 | 3 |
Unique Cell Count | 320 | 264 | 332 | 292 | 340 | 448 | 568 | 560 | 336 | 332 | 327 | 418 | 112 | 199 | 233 | 87 | 101 | 18 | 353 | 297 | 368 | 54 | 143 | 160 |
Labelled Cell Count | 451 | 328 | 443 | 362 | 392 | 568 | 679 | 652 | 436 | 415 | 398 | 484 | 131 | 216 | 240 | 117 | 138 | 26 | 353 | 297 | 368 | 54 | 143 | 160 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.3 | 6.4 | 6.3 | 5.8 | 6.3 | 4.9 | 4.9 | 5.1 | 4.2 | 4.0 | 4.0 | 4.4 | 6.0 | 6.4 | 6.4 | 10.0 | 10.5 | 9.9 | 5.8 | 5.8 | 6.3 |
Std Deviation (1e-4) | 0.9 | 1.1 | 0.7 | 1.0 | 1.3 | 1.0 | 0.9 | 1.0 | 0.8 | 0.6 | 0.7 | 0.8 | 0.6 | 0.9 | 1.1 | 1.7 | 2.0 | 1.7 | 1.6 | 1.4 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.12 | -0.01 | -0.36 | -0.38 | -0.3 | -0.58 | -0.65 | -0.67 | -0.51 | -0.08 | 0.02 | 0.03 | 0.67 | 0.74 | 0.65 | -0.12 | -0.12 | 0.0 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -2.9 | -2.8 | -4.3 | 0 | -8.0 | 0 | 0 | 0 | 0 | -2.3 | -5.1 | -6.7 | 0.1 | -0.5 | 0 |
Bud | -3.3 | -1.9 | -2.2 | 0 | -3.2 | 0 | 0 | 0 | 0 | -1.7 | -4.2 | -4.9 | -1.6 | -1.4 | 0 |
Bud Neck | -1.3 | -0.3 | -0.8 | 0 | -1.0 | 0 | 0 | 0 | 0 | 0 | 0 | -3.1 | 0 | 0 | 0 |
Bud Site | -1.7 | -4.6 | -3.3 | 0 | -7.6 | 0 | 0 | 0 | 0 | -1.0 | -4.9 | -5.5 | -0.7 | -1.7 | 0 |
Cell Periphery | 1.8 | -1.8 | 0 | 0 | -1.6 | 0 | 0 | 0 | 0 | 0 | 0.8 | 0 | 0 | 4.1 | 0 |
Cytoplasm | 3.3 | 4.7 | 2.0 | 3.8 | 4.2 | 0.1 | 0 | 0.8 | 1.5 | 2.7 | 8.2 | 9.6 | -0.5 | -0.3 | -1.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 6.7 | 0 | 6.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 2.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | -1.3 | -3.7 | -1.3 | 0 | -3.8 | 0 | 0 | 0 | 0 | -3.5 | -5.2 | -5.9 | -1.4 | -1.8 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 1.9 | 0 | 0.5 | -0.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |