ORF
Human Ortholog
Description Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; predicted to be palmitoylated
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0 0.06 0.09 0.11 0.21 0.2 0.25 0.29 – 0.21 0.26 0 0 0 0.09 0.13 0.14 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0.05 0 0 – 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0.05 0.19 0.14 0.12 0.1 0.07 – 0.07 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Mitochondria 0.1 0.1 0 0.1 0 0.13 0.13 0.11 0.32 – 0.36 0.22 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.08 0.16 0.12 0.05 0 0 0.05 0 0 – 0 0 0.07 0 0.05 0.05 0.05 0.05 0 0.05 0 0.05 0 0
Nuclear Periphery 0.16 0.07 0.05 0 0 0 0 0 0 – 0 0 0.09 0.1 0.11 0.07 0 0.07 0.13 0 0.07 0 0.06 0.06
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0.05 0 0 0.05 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.81 0.81 0.86 0.8 0.69 0.62 0.54 0.58 0.41 – 0.48 0.47 0.87 0.93 0.9 0.83 0.81 0.79 0.75 0.84 0.82 0.86 0.83 0.8
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 3 0 7 2 1 – 3 6 0 0 0 1 0 1 0 1 1 0 0 0
Bud 0 1 1 1 4 6 8 10 9 – 11 9 1 0 0 0 0 0 0 0 0 0 4 6
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 0 0 1 5 8 3 – 0 5 0 0 0 0 1 2 0 0 0 0 0 0
Cytoplasm 8 3 16 15 30 72 103 91 92 – 66 112 16 13 20 17 16 21 1 1 3 0 0 1
Endoplasmic Reticulum 1 0 1 1 6 4 27 13 11 – 12 32 2 2 3 7 1 4 0 0 2 0 4 3
Endosome 1 0 2 9 53 46 59 38 24 – 23 28 3 8 9 6 0 4 4 3 8 11 20 23
Golgi 0 2 0 0 0 1 7 2 2 – 0 2 0 3 0 8 8 5 1 0 2 0 0 4
Mitochondria 15 14 10 17 11 45 69 39 102 – 113 94 15 4 15 7 3 3 1 1 8 2 4 5
Nucleus 13 23 33 8 9 7 24 15 9 – 8 15 30 19 30 9 6 7 5 8 6 15 16 17
Nuclear Periphery 25 10 13 5 2 4 11 3 7 – 7 3 37 54 72 12 1 11 21 4 20 5 29 32
Nucleolus 3 4 0 2 6 9 16 1 4 – 9 4 3 5 6 3 2 4 1 0 0 2 3 3
Peroxisomes 0 0 0 0 0 2 0 1 0 – 0 2 0 0 1 0 0 0 0 0 0 0 1 0
SpindlePole 0 0 0 0 15 11 19 17 8 – 10 10 0 0 0 2 0 1 0 1 0 0 1 2
Vac/Vac Membrane 125 119 243 132 195 208 274 214 132 – 151 200 374 498 570 152 101 118 126 129 253 266 437 410
Unique Cell Count 154 147 282 164 281 336 512 370 322 317 424 429 537 630 183 125 149 168 155 311 309 527 515
Labelled Cell Count 191 176 320 190 334 416 629 454 404 413 523 481 606 726 224 139 181 168 155 311 309 527 515


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.8 7.8 7.7 6.8 7.6 6.6 6.8 7.0 6.2 – 6.1 6.4 8.7 8.9 9.0 12.5 12.5 12.5 7.3 7.6 8.4
Std Deviation (1e-4) 1.4 1.9 1.1 1.2 1.3 1.5 1.3 1.3 1.1 – 1.3 1.4 5.0 1.4 1.6 3.1 3.0 3.4 1.5 1.5 1.9
Intensity Change (Log2) – – – -0.19 -0.01 -0.22 -0.19 -0.15 -0.31 – -0.35 -0.26 0.17 0.2 0.22 0.7 0.7 0.7 -0.07 -0.02 0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 2.7 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 1.4 2.2 5.6 5.5 6.5 7.3 – 5.4 7.0 -1.2 -2.4 -1.8 1.5 2.5 3.0
Endoplasmic Reticulum 0 0 0 3.6 2.8 2.7 – 2.9 4.4 0 0 0 0 0 0
Endosome 0 7.3 6.0 5.5 5.0 4.1 – 4.0 3.8 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 2.9 0.2 4.3 4.5 3.4 8.9 – 9.7 6.8 0 0 -1.0 0.2 0 0
Nucleus -2.4 -3.8 -4.8 -3.7 -3.7 -4.3 – -4.4 -4.2 -2.2 -4.6 -3.8 -2.5 -2.2 -2.4
Nuclear Periphery -0.8 -2.9 -2.6 -1.9 -3.1 -1.7 – -1.6 -3.4 2.0 2.7 3.3 0.9 0 1.2
Nucleolus 0 0 0 3.0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 3.9 3.1 3.3 3.6 0 – 0 0 0 0 0 0 0 0
Vacuole -1.6 -4.8 -6.8 -9.2 -7.8 -11.4 – -9.9 -10.5 0.4 3.0 1.9 -0.9 -1.4 -1.9