| Standard name | |
|---|---|
| Human Ortholog | |
| Description | Homodimeric subunit of autophagy-specific PtdIns-3-kinase complex I; required for the integrity of the active PtdIns-3-kinase complex I by maintaining an association between Vps15p-Vps34p and Atg14p-Vps30p subcomplexes; localizes to the pre-autophagosomal structure (PAS) in an Atg14p-dependent manner; ATG38 is non-essential but is required for macroautophagy |
Micrographs
Sub-cellular Localization
Yeast GFP Assignment
Protein Abundance
Localization Change
External localization resources
| ensLOC | DeepLoc | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
| Cortical Patches | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 3 | 3 |
| Bud | 0 | 0 | 0 | 3 | 1 | 3 | 5 | 2 | 5 | 8 | 8 | 4 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 8 | 8 |
| Bud Neck | 0 | 0 | 3 | 5 | 6 | 9 | 14 | 21 | 3 | 10 | 2 | 10 | 7 | 7 | 2 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 1 |
| Bud Site | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
| Cell Periphery | 0 | 0 | 3 | 0 | 0 | 1 | 1 | 2 | 0 | 0 | 1 | 3 | 0 | 3 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| Cytoplasm | 105 | 80 | 174 | 136 | 260 | 280 | 323 | 374 | 176 | 306 | 160 | 339 | 158 | 317 | 375 | 44 | 26 | 49 | 62 | 64 | 107 | 105 | 214 | 206 |
| Endoplasmic Reticulum | 0 | 4 | 5 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 4 | 1 | 7 | 3 | 0 | 1 | 0 | 3 | 3 |
| Endosome | 1 | 0 | 2 | 3 | 7 | 4 | 11 | 3 | 0 | 3 | 2 | 7 | 2 | 4 | 17 | 7 | 8 | 21 | 18 | 11 | 18 | 6 | 23 | 45 |
| Golgi | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 3 | 0 | 0 | 0 | 0 | 2 |
| Mitochondria | 6 | 31 | 0 | 2 | 1 | 16 | 28 | 21 | 63 | 64 | 93 | 117 | 1 | 0 | 0 | 4 | 4 | 1 | 14 | 15 | 33 | 11 | 20 | 31 |
| Nucleus | 1 | 1 | 0 | 1 | 0 | 4 | 2 | 4 | 2 | 3 | 1 | 1 | 1 | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 0 | 1 | 2 | 3 |
| Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 1 | 0 | 2 | 2 |
| Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| SpindlePole | 1 | 0 | 2 | 31 | 30 | 12 | 22 | 26 | 5 | 25 | 8 | 35 | 3 | 12 | 12 | 0 | 3 | 7 | 3 | 0 | 1 | 1 | 12 | 15 |
| Vac/Vac Membrane | 12 | 4 | 1 | 1 | 4 | 5 | 5 | 4 | 1 | 1 | 2 | 4 | 1 | 3 | 12 | 15 | 19 | 33 | 47 | 30 | 19 | 21 | 68 | 97 |
| Unique Cell Count | 124 | 106 | 184 | 173 | 302 | 313 | 376 | 418 | 209 | 366 | 222 | 445 | 165 | 344 | 406 | 62 | 53 | 97 | 166 | 128 | 193 | 158 | 374 | 432 |
| Labelled Cell Count | 128 | 120 | 190 | 183 | 312 | 335 | 413 | 460 | 255 | 421 | 277 | 526 | 173 | 354 | 425 | 76 | 64 | 121 | 166 | 128 | 193 | 158 | 374 | 432 |
Yeast GFP Assignment
Protein Abundance
| Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Cell GFP Intensity (1e-4) | 4.1 | 3.2 | 5.1 | 4.8 | 5.2 | 4.2 | 4.0 | 4.3 | 3.5 | 3.6 | 3.6 | 3.7 | 4.7 | 4.7 | 4.7 | 5.1 | 6.1 | 6.7 | 5.3 | 5.6 | 5.7 |
| Std Deviation (1e-4) | 0.7 | 0.4 | 0.9 | 1.5 | 1.4 | 1.4 | 1.2 | 1.4 | 1.4 | 1.1 | 1.3 | 1.4 | 1.0 | 0.9 | 1.1 | 1.5 | 1.5 | 1.6 | 1.5 | 1.6 | 1.8 |
| Intensity Change (Log2) | – | – | – | -0.07 | 0.04 | -0.28 | -0.34 | -0.22 | -0.54 | -0.49 | -0.51 | -0.44 | -0.11 | -0.1 | -0.1 | 0.01 | 0.28 | 0.4 | 0.05 | 0.16 | 0.17 |
Localization Change
| Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
| Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
| Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
| SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
| Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
| Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
| Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
| Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 8.2 |
External localization resources
