Standard name | |
---|---|
Human Ortholog | |
Description | Dock family protein (Dedicator Of CytoKinesis), homolog of human DOCK1; upstream component for regulation through the small GTPase Rho5p; may form a complex with Lmo1p that acts as a GEF for Rho5p; interacts with Ino4p; cytoplasmic protein that relocates to mitochondria under oxidative stress; implicated in mitophagy; not an essential protein; DOCK proteins act as guanine nucleotide exchange factors |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 5 | 4 | 4 | 6 | 4 | 14 | 9 | 4 | 2 | 4 | 2 | 4 | 7 | 6 | 5 | – | – | – | 31 | 15 | 56 | 28 | 54 | 85 |
Bud | 2 | 2 | 22 | 20 | 19 | 46 | 49 | 50 | 31 | 56 | 30 | 41 | 7 | 4 | 21 | – | – | – | 7 | 1 | 7 | 4 | 11 | 7 |
Bud Neck | 0 | 0 | 11 | 7 | 13 | 18 | 8 | 20 | 9 | 11 | 8 | 9 | 0 | 0 | 1 | – | – | – | 12 | 14 | 16 | 10 | 17 | 27 |
Bud Site | 0 | 0 | 0 | 1 | 9 | 10 | 10 | 17 | 13 | 11 | 6 | 4 | 0 | 0 | 1 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 31 | 12 | 16 | 22 | 19 | 24 | 17 | 19 | 41 | 26 | 31 | 24 | 63 | 95 | 111 | – | – | – | 15 | 11 | 41 | 16 | 45 | 94 |
Cytoplasm | 274 | 185 | 509 | 364 | 501 | 596 | 626 | 671 | 475 | 538 | 318 | 325 | 386 | 456 | 535 | – | – | – | 190 | 127 | 269 | 223 | 262 | 254 |
Endoplasmic Reticulum | 1 | 5 | 2 | 1 | 0 | 2 | 1 | 1 | 1 | 1 | 1 | 2 | 1 | 3 | 4 | – | – | – | 38 | 6 | 16 | 5 | 25 | 32 |
Endosome | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 4 | – | – | – | 7 | 4 | 7 | 2 | 2 | 5 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 4 | 0 | 2 | 1 | 0 | 1 |
Mitochondria | 11 | 4 | 5 | 25 | 34 | 108 | 162 | 153 | 159 | 202 | 119 | 141 | 0 | 1 | 4 | – | – | – | 17 | 9 | 72 | 17 | 24 | 29 |
Nucleus | 0 | 1 | 0 | 0 | 0 | 2 | 1 | 4 | 2 | 5 | 1 | 4 | 0 | 1 | 1 | – | – | – | 0 | 1 | 0 | 1 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | – | – | – | 1 | 0 | 1 | 0 | 0 | 1 |
SpindlePole | 5 | 0 | 6 | 1 | 14 | 11 | 19 | 26 | 19 | 15 | 5 | 4 | 2 | 0 | 2 | – | – | – | 1 | 0 | 3 | 0 | 0 | 2 |
Vac/Vac Membrane | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 1 | 1 | 0 | 3 | 0 | 1 | 2 | – | – | – | 3 | 8 | 6 | 6 | 7 | 6 |
Unique Cell Count | 305 | 197 | 532 | 385 | 548 | 704 | 784 | 836 | 601 | 705 | 428 | 450 | 397 | 471 | 557 | 338 | 206 | 521 | 323 | 462 | 557 | |||
Labelled Cell Count | 342 | 214 | 575 | 447 | 613 | 831 | 903 | 971 | 753 | 871 | 521 | 562 | 467 | 570 | 693 | 338 | 206 | 521 | 323 | 462 | 557 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.3 | 5.4 | 3.8 | 3.6 | 3.8 | 3.5 | 3.5 | 3.6 | 3.2 | 3.1 | 3.2 | 3.2 | 5.6 | 5.7 | 5.1 | – | – | – | 5.7 | 5.9 | 6.2 |
Std Deviation (1e-4) | 1.0 | 0.7 | 0.8 | 0.9 | 1.1 | 0.8 | 0.9 | 1.3 | 1.1 | 0.9 | 0.9 | 1.1 | 0.9 | 1.1 | 1.0 | – | – | – | 1.7 | 1.5 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.09 | 0.01 | -0.11 | -0.13 | -0.06 | -0.25 | -0.28 | -0.26 | -0.26 | 0.57 | 0.59 | 0.42 | – | – | – | 0.6 | 0.63 | 0.72 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -2.1 | -3.3 | -0.3 | – | – | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -3.1 | -3.0 | – | – | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 7.0 | 8.6 | 8.7 | – | – | – |
Cytoplasm | -0.8 | -2.8 | -6.2 | -8.2 | -8.1 | -8.3 | -9.4 | -9.5 | -10.2 | 1.2 | 0.9 | 0.3 | – | – | – |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |