Standard name
Human Ortholog
Description Putative 1,3-beta-glucanosyltransferase; has similarity go other GAS family members; low abundance, possibly inactive member of the GAS family of GPI-containing proteins; localizes to the cell wall; mRNA induced during sporulation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0.08 0.09 0.05 0.08 0.1 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0.06 0 0.06 0 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0.05 0.09 0.07 0.05 0 0.08 0.06 0 0.05 0.07 0.08 0.11 0 0 0 0 0 0 0 0 0
Cytoplasm 0.63 0.58 0.66 0.55 0.45 0.37 0.38 0.43 0.47 0.53 0.69 0.68 0.39 0.58 0.64 0.36 0.44 0.35 0 0.08 0.08 0.07 0.11 0.05
Endoplasmic Reticulum 0.32 0.39 0.28 0.44 0.43 0.48 0.47 0.34 0.36 0.24 0.14 0.13 0.57 0.4 0.31 0 0 0 0.64 0.63 0.66 0.81 0.74 0.59
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0 0 0 0 0.1 0.08 0.09 0.11 0.1 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.23 0.18 0.22 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0.05 0 0 0.08 0.06 0.09 0.1 0.05 0.1 0.08 0.13 0 0 0 0.62 0.6 0.55 0.3 0.21 0.17 0.06 0.1 0.26
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 3 8 24 28 19 32 35 21 12 10 7 9 6 2 0 0 0 3 4 3 2 9
Bud 0 1 7 2 5 20 10 26 13 24 11 10 3 4 3 1 0 0 0 3 3 2 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 3
Bud Site 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 4 6 10 26 22 20 15 29 24 9 14 13 31 41 0 1 0 0 0 0 0 2 1
Cytoplasm 12 88 127 110 133 121 161 179 173 222 167 192 71 222 244 89 53 34 0 14 17 9 34 19
Endoplasmic Reticulum 6 60 53 88 129 156 198 142 132 100 33 36 104 155 120 4 4 2 39 110 151 118 236 210
Endosome 0 1 1 0 9 6 5 12 0 7 2 1 2 7 4 6 2 0 0 0 2 0 2 5
Golgi 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 5 5 1 0 3 0 0 2 3
Mitochondria 1 3 3 2 2 14 44 34 32 47 25 27 0 0 1 0 1 0 0 1 3 0 1 2
Nucleus 0 0 0 0 0 0 0 0 0 6 0 4 1 0 1 56 22 21 0 0 0 0 0 0
Nuclear Periphery 0 1 0 1 0 2 5 4 4 3 3 3 0 0 1 1 0 0 0 0 1 0 0 0
Nucleolus 0 0 0 0 0 0 1 1 0 0 0 0 1 0 1 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 1
SpindlePole 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 7 8 2 25 21 39 41 19 41 19 36 2 2 9 152 73 53 18 36 39 8 31 90
Unique Cell Count 19 152 192 200 298 328 420 421 368 419 243 282 184 383 384 244 121 97 62 177 229 146 321 353
Labelled Cell Count 20 166 208 223 353 391 502 487 437 498 281 333 204 430 431 317 161 111 62 177 229 146 321 353


Cytoplasm, Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.2 8.7 7.2 7.7 9.4 8.5 7.7 8.0 7.9 7.3 6.9 6.9 9.5 8.8 8.0 12.7 14.2 16.2 8.5 8.2 8.3
Std Deviation (1e-4) 1.3 1.9 1.6 1.5 1.8 1.6 1.8 1.6 1.6 1.6 1.5 1.4 2.2 1.9 1.8 2.8 3.2 3.3 1.9 1.4 1.5
Intensity Change (Log2) – – – 0.09 0.39 0.24 0.09 0.15 0.13 0.01 -0.07 -0.07 0.41 0.29 0.15 0.82 0.98 1.17 0.23 0.18 0.2


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.5 3.1 3.2 1.8 3.0 3.5 2.0 1.9 1.3 0 0 0 0 0 0
Bud 0 0 1.2 -0.9 1.3 -0.1 1.1 0.5 -0.1 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.9 2.4 1.7 0.9 0.3 2.2 1.4 0.3 1.0 1.7 2.3 3.1 0 0 0
Cytoplasm -2.3 -4.7 -6.4 -6.4 -5.4 -4.3 -3.1 0.6 0.4 -5.4 -1.9 -0.6 -6.2 -3.9 -5.0
Endoplasmic Reticulum 3.4 3.5 4.5 4.6 1.5 2.0 -1.0 -3.6 -4.1 5.7 3.0 0.9 -8.0 -5.4 -5.2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 1.7 3.8 3.1 3.3 4.0 3.7 3.5 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 7.1 6.1 6.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 1.8 1.1 2.2 2.4 0.5 2.4 1.6 3.2 0 0 -1.2 12.5 11.0 9.9