Standard name
Human Ortholog
Description Putative mitochondrial ribosomal protein of the small subunit; has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency; localizes to vacuole in response to H2O2
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0
Bud 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.1 0.09 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0.07 0.1 0 0 0 0 0.05 0 0 0
Golgi 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0.1 0.13 0.11 0.23 0.42 0.45 0.06 0.07 0 0.11 0.08 0.12
Mitochondria 0.91 0.85 0.98 0.96 0.88 0.93 0.92 0.89 0.94 0.94 0.96 0.97 0.87 0.9 0.82 0.13 0.06 0.09 0.8 0.76 0.83 0.66 0.66 0.66
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.05 0 0 0.05 0.07 0.13 0.15 0.09 0.15 0.1 0.1 0.13 0 0 0 0 0.08 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0.07 0.08 0.1 0.1 0.07 0.09 0.06 0.07 0 0 0 0.07 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.1 0.13 0.22 0.16 0 0 0 0.07 0.09 0.08
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.16 0.17 0.06 0 0 0 0 0.05 0.05 0 0 0 0.09 0 0.1 0.23 0.06 0.15 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 4 1 0 0 1 0 1 1 1 1 1 0 0 0 5 1 3 6 21 2 9 13 11
Bud 7 6 31 9 16 24 15 25 19 14 5 14 2 4 3 7 3 3 2 4 1 0 3 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 2
Bud Site 0 0 6 1 1 6 5 2 4 3 10 4 0 0 0 0 0 0 – – – – – –
Cell Periphery 23 30 18 9 4 3 0 2 6 3 3 10 9 6 11 16 5 8 0 0 0 0 0 2
Cytoplasm 3 14 7 10 12 9 11 18 11 11 8 6 7 9 8 14 3 4 1 2 1 0 1 0
Endoplasmic Reticulum 5 5 2 4 3 2 2 5 5 2 8 6 1 6 2 7 2 0 1 0 2 0 0 0
Endosome 5 19 2 1 30 3 1 9 2 4 0 0 10 13 23 18 2 3 17 27 26 5 11 8
Golgi 25 30 0 0 4 3 1 2 0 0 4 0 26 44 38 41 21 38 27 47 17 17 25 37
Mitochondria 422 554 549 312 512 589 823 797 772 896 554 650 218 294 274 23 3 8 383 527 482 106 205 212
Nucleus 5 1 1 0 3 1 4 10 2 3 1 2 0 0 0 3 0 1 0 0 0 1 1 0
Nuclear Periphery 22 20 24 15 40 83 130 77 125 96 59 84 7 10 9 1 4 0 2 4 2 0 3 1
Nucleolus 10 13 23 9 39 52 87 89 59 86 37 44 3 8 6 12 0 0 1 2 0 0 4 2
Peroxisomes 4 23 5 1 22 6 10 7 6 7 3 3 11 19 32 24 11 14 11 12 25 10 26 23
SpindlePole 2 2 2 0 14 13 5 10 4 8 3 2 3 2 4 14 2 3 0 6 1 1 3 0
Vac/Vac Membrane 76 110 36 14 24 18 25 41 37 28 21 12 23 13 33 41 3 13 18 25 8 2 10 13
Unique Cell Count 463 650 560 326 580 634 891 900 817 956 577 671 250 328 334 180 50 85 477 693 579 161 314 319
Labelled Cell Count 612 831 707 385 724 813 1119 1095 1053 1162 717 838 320 428 443 226 60 98 477 693 579 161 314 319


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.1 9.0 4.9 6.2 7.0 6.2 6.4 6.8 6.6 6.8 6.9 6.9 9.6 9.4 9.5 9.5 9.4 9.5 9.9 10.9 11.3
Std Deviation (1e-4) 1.4 1.8 1.0 1.6 1.4 1.5 1.6 1.7 1.8 1.9 2.0 1.9 1.6 1.7 1.7 2.5 2.4 2.7 2.2 2.3 2.5
Intensity Change (Log2) – – – 0.35 0.51 0.33 0.37 0.47 0.42 0.47 0.5 0.5 0.97 0.94 0.95 0.96 0.93 0.96 1.01 1.16 1.21


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud -1.9 -2.4 -1.4 -4.1 -2.7 -3.1 -4.5 -4.5 -3.2 -3.1 -3.2 -3.5 -0.9 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 -0.2 0 0 0 0 0.9 0 0 0 0 0 0 0
Cell Periphery -0.4 -3.1 -3.6 -5.4 -4.8 -3.5 -4.7 -3.4 -2.0 0.3 -1.2 0.1 3.2 0 0
Cytoplasm 1.9 1.1 0.3 0 1.1 0.2 -0.2 0.2 -0.6 0 1.6 1.3 4.6 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 4.9 0 0 0 0 0 0 0 0 4.0 5.7 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 7.8 8.9 8.2 11.6 0 16.3
Mitochondria -2.0 -6.5 -4.2 -4.6 -6.6 -3.3 -3.8 -2.0 -1.3 -6.4 -5.5 -8.6 -23.8 -21.3 -22.2
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.2 1.9 5.3 6.2 3.1 6.5 4.0 3.8 5.1 -1.0 -0.9 -1.2 -2.4 0 0
Nucleolus -1.0 1.9 2.9 4.0 4.0 2.4 3.6 1.7 1.9 -2.2 -1.3 -1.9 1.4 0 0
Peroxisomes 0 3.2 0.1 0.4 0 0 0 0 0 0 4.3 6.3 7.5 0 0
SpindlePole 0 3.0 2.6 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.3 -1.7 -3.0 -3.3 -1.6 -1.5 -3.3 -2.2 -4.2 1.4 -1.6 1.9 6.2 0 2.9