Standard name
Human Ortholog
Description Protein required for uridine thiolation of Lys(UUU) and Glu(UUC) tRNAs; required for the thiolation of uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Cytoplasm 0.96 0.99 0.99 0.89 0.99 0.96 0.76 0.84 0.73 0.72 0.55 0.57 1.0 0.99 1.0 0.78 0.8 0.85 0.96 0.94 0.91 0.92 0.94 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.14 0.08 0.05 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Mitochondria 0 0 0 0.17 0 0.26 0.48 0.33 0.5 0.56 0.66 0.64 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0.06 0.08 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 2 0 0 1 0 0 0 1
Bud 0 1 0 0 0 1 1 3 2 5 2 1 2 1 1 0 0 0 0 2 0 0 0 0
Bud Neck 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 8 1 2 2 2 0 0 0 2 5 5 3 2 4 6 9 3 0 0 0 0 0 0
Cytoplasm 301 386 68 48 93 98 99 139 48 92 80 74 180 190 194 138 123 94 294 372 79 109 135 162
Endoplasmic Reticulum 2 3 1 0 0 0 0 1 0 1 3 1 0 0 0 7 4 8 1 2 0 2 0 3
Endosome 0 0 0 1 0 0 1 1 0 1 2 0 1 0 0 25 13 6 4 3 0 1 2 1
Golgi 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 4 5 0 1 1 0 0 0
Mitochondria 10 0 2 9 3 27 62 54 33 71 97 82 1 1 0 2 4 3 1 1 0 0 0 1
Nucleus 1 0 0 0 0 0 1 0 0 0 0 2 0 0 2 0 4 1 0 0 0 0 0 0
Nuclear Periphery 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 3 3 0 0 0 0 0 0 0 7 1 3 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 9 9 1 0 0 0 0 1 0
Vac/Vac Membrane 1 1 0 3 0 0 4 2 0 3 1 2 0 0 0 10 12 5 0 7 0 0 0 2
Unique Cell Count 312 390 69 54 94 102 130 165 66 127 146 129 180 191 194 176 153 111 308 397 87 118 144 177
Labelled Cell Count 318 399 72 63 98 129 172 204 83 176 192 167 187 194 201 205 185 129 308 397 87 118 144 177


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.9 7.3 6.1 6.2 5.5 4.6 3.9 4.5 4.4 3.6 4.1 3.8 6.7 6.2 6.2 8.2 9.3 9.7 6.2 6.3 7.1
Std Deviation (1e-4) 0.6 1.0 1.3 2.1 1.7 0.9 1.3 2.1 2.2 0.7 1.7 1.2 0.9 1.2 1.2 1.5 1.7 2.4 1.5 1.5 1.2
Intensity Change (Log2) – – – 0.02 -0.17 -0.41 -0.65 -0.45 -0.49 -0.75 -0.59 -0.69 0.12 0.01 0.01 0.42 0.59 0.66 0.01 0.04 0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -2.3 0.2 -0.9 -4.1 -3.1 -4.3 -4.5 -6.5 -6.1 1.6 0.8 1.7 -3.9 -3.6 -3.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 3.3 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0