Standard name
Human Ortholog
Description Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain an S1 RNA binding domain; human homolog EXOSC1 partially complements yeast csl4 null mutant, and can complement inviability of strain in which expression of CSL4 is repressed
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.12 0.07 0.07 0 0 0 0 0 0
Cytoplasm 0 0 0.08 0.08 0.05 0.07 0.07 – 0.06 0.07 0 0.06 0.13 0.18 0.21 0.13 0.13 0.14 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.15 0.28 0.22 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.09 0 0 – 0.25 0.1 0.18 0.17 0 0 0 0 0.06 0.07 0 0 0 0 0 0
Nucleus 0.65 0.61 0.62 0.84 0.68 0.69 0.65 – 0.62 0.68 0.67 0.65 0.65 0.61 0.67 0.18 0.16 0.15 0.43 0.52 0.41 0.38 0.31 0.27
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.07 0 0.06 0 0 0 0 0 0
Nucleolus 0.29 0.26 0.22 0 0.07 0.07 0.08 – 0.13 0.09 0.08 0.14 0.18 0.13 0.07 0.07 0.07 0 0.41 0.32 0.42 0.3 0.34 0.37
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0.08 0.05 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.05
Vac/Vac Membrane 0.12 0.17 0.12 0.1 0.16 0.2 0.21 – 0.11 0.12 0.11 0.08 0.07 0.1 0.06 0.22 0.19 0.26 0 0.07 0 0.17 0.19 0.18
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 5 1 6 0 0 0 1 1 1
Bud 0 0 0 0 0 0 2 – 4 4 1 3 0 0 0 8 4 5 15 7 2 13 14 15
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 3
Bud Site 0 0 0 0 0 2 0 – 6 2 5 8 0 0 0 0 1 0 – – – – – –
Cell Periphery 0 1 1 0 0 0 0 – 0 0 0 1 0 0 0 38 26 23 0 0 0 0 0 0
Cytoplasm 8 15 16 15 9 19 24 – 21 21 12 21 54 109 127 43 46 47 0 5 0 10 8 7
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 9 9 14 0 0 0 2 2 1
Endosome 4 11 6 1 2 5 5 – 1 2 3 5 8 18 16 8 7 11 15 14 11 19 22 27
Golgi 1 1 0 0 0 1 2 – 0 0 0 0 1 4 1 48 96 78 2 3 2 3 2 3
Mitochondria 2 7 0 1 16 2 10 – 86 33 58 58 1 0 0 14 22 24 1 2 0 3 3 3
Nucleus 246 303 128 166 117 200 238 – 213 215 215 227 264 365 404 56 55 52 204 313 112 231 214 184
Nuclear Periphery 0 0 0 0 0 0 0 – 3 0 1 1 0 2 1 22 5 21 1 0 2 5 8 12
Nucleolus 110 131 46 4 12 19 29 – 43 30 27 50 74 76 44 22 23 9 197 188 113 182 235 251
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 1 0 1 18 29 19 0 0 0 0 0 0
SpindlePole 0 3 0 0 0 0 1 – 0 0 0 0 1 1 2 4 6 8 16 15 12 20 30 35
Vac/Vac Membrane 44 86 24 19 28 59 76 – 37 38 35 29 27 62 37 70 67 89 21 39 10 100 132 119
Unique Cell Count 378 495 207 197 171 290 364 342 316 321 351 405 601 607 319 347 348 478 598 272 604 691 680
Labelled Cell Count 416 558 221 206 184 307 387 414 345 357 403 431 637 633 365 397 406 478 598 272 604 691 680


Cytoplasm, Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.8 11.1 14.2 13.5 8.2 10.5 9.3 – 8.1 8.9 8.6 8.3 13.6 13.8 12.7 10.2 12.4 11.6 14.6 14.6 14.5
Std Deviation (1e-4) 3.0 2.2 3.8 4.1 2.4 3.0 2.5 – 2.4 2.4 2.5 2.3 4.0 4.8 4.0 2.7 3.7 3.6 4.6 3.7 4.7
Intensity Change (Log2) – – – -0.07 -0.79 -0.43 -0.61 – -0.8 -0.66 -0.72 -0.77 -0.06 -0.04 -0.15 -0.47 -0.2 -0.29 0.04 0.05 0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 4.9 3.7 3.4
Cytoplasm 0 -1.0 -0.5 -0.5 – -0.7 -0.5 -2.0 -0.8 2.1 3.6 4.3 2.0 2.0 2.1
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 2.9
Endosome 0 0 0 0 – 0 0 0 0 0 0.1 -0.2 -0.3 0 0.2
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 5.9 8.3 7.3
Mitochondria 0 4.5 0 0 – 7.9 4.8 6.5 6.2 0 0 0 3.1 3.7 3.9
Nucleus 5.1 1.3 1.7 0.8 – 0.1 1.5 1.2 0.7 0.8 -0.3 1.2 -10.4 -11.1 -11.4
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 3.9 0 3.6
Nucleolus -6.2 -4.1 -5.1 -4.8 – -3.0 -4.0 -4.5 -2.4 -1.2 -3.3 -5.9 -5.1 -5.4 -7.5
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 3.5 4.3 3.4
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole -0.6 1.3 2.6 2.8 – -0.3 0.1 -0.2 -1.3 -2.1 -0.5 -2.6 3.0 2.4 4.0