Standard name | |
---|---|
Human Ortholog | |
Description | Phosphatidylinositol 3,5-bisphosphate (PtdIns[3,5]P) phosphatase; required for efficient mating and response to osmotic shock; physically associates with and regulated by Vac14p; contains a SAC1-like domain; homologous to human FIG4, which is associated with CMT4J, a form of Charcot-Marie-Tooth disorder |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 0 | 6 | 0 | 0 | 0 | 0 | 2 | 0 | – | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 3 | 0 | 1 | 3 |
Bud | 4 | 2 | 11 | 2 | 5 | 4 | 1 | 7 | 13 | – | 15 | 8 | 3 | 3 | 7 | 2 | 0 | 2 | 1 | 1 | 1 | 5 | 6 | 8 |
Bud Neck | 0 | 0 | 10 | 1 | 1 | 1 | 7 | 12 | 3 | – | 4 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | – | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 85 | 101 | 179 | 138 | 187 | 240 | 257 | 356 | 193 | – | 136 | 250 | 172 | 286 | 351 | 30 | 14 | 4 | 29 | 32 | 64 | 22 | 31 | 30 |
Endoplasmic Reticulum | 6 | 6 | 2 | 4 | 1 | 1 | 2 | 0 | 0 | – | 1 | 1 | 1 | 0 | 0 | 3 | 1 | 0 | 5 | 1 | 4 | 6 | 7 | 11 |
Endosome | 27 | 8 | 12 | 3 | 11 | 11 | 1 | 4 | 1 | – | 2 | 3 | 30 | 58 | 57 | 8 | 1 | 6 | 77 | 44 | 54 | 46 | 59 | 50 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 2 | 0 | 2 | 3 |
Mitochondria | 32 | 25 | 5 | 4 | 3 | 35 | 31 | 30 | 83 | – | 92 | 55 | 9 | 8 | 8 | 4 | 0 | 1 | 27 | 20 | 44 | 15 | 21 | 19 |
Nucleus | 0 | 0 | 0 | 1 | 1 | 3 | 6 | 5 | 4 | – | 1 | 4 | 0 | 1 | 2 | 0 | 1 | 1 | 1 | 1 | 2 | 1 | 1 | 0 |
Nuclear Periphery | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 2 | 0 | 3 | 2 | 3 | 4 |
Nucleolus | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 |
Peroxisomes | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 3 | 3 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 7 | 0 | 7 | 4 | 11 | 6 | 4 | 15 | 3 | – | 1 | 5 | 1 | 1 | 7 | 2 | 0 | 1 | 5 | 2 | 5 | 1 | 4 | 2 |
Vac/Vac Membrane | 27 | 59 | 24 | 9 | 19 | 13 | 12 | 11 | 16 | – | 13 | 7 | 63 | 142 | 90 | 14 | 3 | 9 | 72 | 113 | 92 | 122 | 168 | 195 |
Unique Cell Count | 161 | 177 | 227 | 161 | 227 | 288 | 293 | 405 | 262 | 197 | 292 | 252 | 414 | 481 | 55 | 18 | 16 | 232 | 225 | 288 | 232 | 316 | 340 | |
Labelled Cell Count | 195 | 203 | 258 | 166 | 239 | 315 | 322 | 443 | 317 | 266 | 341 | 280 | 503 | 528 | 65 | 20 | 24 | 232 | 225 | 288 | 232 | 316 | 340 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.5 | 4.5 | 5.2 | 4.6 | 5.1 | 4.1 | 4.0 | 4.4 | 3.7 | – | 3.5 | 3.9 | 4.8 | 4.9 | 4.7 | 7.3 | 6.3 | 6.6 | 4.5 | 4.8 | 5.4 |
Std Deviation (1e-4) | 0.8 | 0.6 | 1.2 | 1.1 | 1.6 | 1.4 | 1.1 | 1.1 | 1.1 | – | 1.1 | 1.3 | 1.1 | 1.5 | 1.3 | 2.3 | 0.9 | 1.1 | 1.6 | 2.1 | 3.5 |
Intensity Change (Log2) | – | – | – | -0.17 | -0.01 | -0.35 | -0.37 | -0.25 | -0.49 | – | -0.57 | -0.39 | -0.1 | -0.07 | -0.13 | 0.49 | 0.29 | 0.35 | -0.21 | -0.11 | 0.06 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 2.9 | – | – |