ORF
Human Ortholog
Description Putative protein of unknown function; deletion confers reduced fitness in saline
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.98 0.99 0.98 0.92 0.84 0.83 0.75 0.76 0.63 0.68 1.0 0.99 1.0 0.95 0.9 0.98 0.91 0.93 0.91 0.95 0.88 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.08 0.05 0.27 0.37 0.32 0.4 0.45 0.59 0.54 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 0 2
Bud 1 1 1 0 2 7 27 12 8 10 11 9 0 0 0 0 0 0 0 4 4 1 6 3
Bud Neck 0 0 1 0 0 0 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 1
Bud Site 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 3 3 1 1 7 3 3 3 3 1 1 1 1 3 6 4 0 0 0 0 0 0
Cytoplasm 161 300 321 275 311 421 564 429 345 340 232 246 211 253 352 81 121 96 185 280 283 208 279 299
Endoplasmic Reticulum 0 2 4 1 2 1 0 0 2 0 1 2 0 0 1 6 5 1 3 0 3 0 2 0
Endosome 0 0 1 0 0 2 0 1 3 2 0 2 0 1 0 1 3 0 1 3 3 1 3 3
Golgi 0 0 1 0 0 1 0 0 0 0 0 0 0 1 0 1 1 1 0 2 2 0 1 1
Mitochondria 5 10 0 22 17 123 249 163 184 199 217 195 0 0 1 3 4 1 6 4 2 0 2 2
Nucleus 0 0 0 0 0 0 5 1 1 0 2 2 1 4 0 1 3 1 0 0 1 1 4 2
Nuclear Periphery 1 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 1 0
Nucleolus 0 0 1 1 0 0 1 0 0 1 0 1 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
SpindlePole 0 0 0 0 2 0 1 2 2 2 3 4 0 0 0 0 0 0 0 1 1 0 3 2
Vac/Vac Membrane 0 0 0 0 0 6 20 6 18 3 14 8 0 0 1 0 6 0 0 2 2 0 3 1
Unique Cell Count 165 305 326 278 318 460 674 514 459 445 367 361 212 255 353 85 134 98 203 302 313 218 319 324
Labelled Cell Count 169 313 333 302 335 562 878 618 566 560 484 471 213 260 356 96 149 105 203 302 313 218 319 324


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 6.0 5.2 4.5 4.8 3.8 3.6 3.4 3.4 3.2 3.0 3.1 6.6 6.7 6.4 9.1 8.6 8.5 6.5 7.2 7.1
Std Deviation (1e-4) 1.0 1.4 1.5 1.2 1.6 1.5 1.4 1.0 1.2 0.9 1.0 1.4 1.4 1.4 1.3 1.7 2.0 1.7 1.5 1.7 1.5
Intensity Change (Log2) – – – -0.2 -0.13 -0.47 -0.55 -0.63 -0.6 -0.69 -0.81 -0.75 0.34 0.36 0.28 0.8 0.73 0.71 0.32 0.46 0.43


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.5 -0.6 -4.2 -6.9 -6.9 -9.0 -8.7 -11.5 -10.5 1.1 0.8 1.7 -1.8 -4.1 -0.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 3.1 0 3.6 0 3.6 0 0 0 0 0 0 0