Standard name
Human Ortholog
Description Receptor protein for the cytoplasm-to-vacuole targeting (Cvt) pathway; delivers cargo proteins aminopeptidase I (Ape1p) and alpha-mannosidase (Ams1p) to the phagophore assembly site for packaging into Cvt vesicles; interaction with Atg19p during the Cvt pathway requires phosphorylation by Hrr25p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0.05 0 0.09 0.05 0.07 0.11 0.14 0.12 0.12 0.19 0 0 0 0.05 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0.08 0.06 0.07 0.05 0.09 0.06 0 0.06 0.06 0.07 0 0.06 0.11 0.05 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0.05 0.06 0.06 0.06 0.07 0 0.05 0 0 0 0 0.06 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.73 0.75 0.8 0.88 0.57 0.48 0.4 0.35 0.29 0.34 0.26 0.39 0.75 0.73 0.77 0.35 0.37 0.36 0.49 0.57 0.5 0.61 0.58 0.61
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.08 0.05 0 0.05 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.15 0 0 0.11 0.06 0.12 0.24 0.23 0.27 0.3 0.36 0.36 0 0 0 0.06 0 0 0.11 0.07 0.2 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.06 0 0 0 0.06 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0.07 0.07 0 0 0 0.06 0 0 0 0 0 0 0.05 0 0 0 0 0 0
SpindlePole 0.17 0.16 0.11 0.08 0.36 0.39 0.42 0.48 0.45 0.38 0.38 0.32 0.23 0.24 0.2 0.58 0.56 0.54 0.2 0.25 0.13 0.22 0.24 0.23
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 1 0 1 0 2 0 0 0 0 1 0 0 0 0 0 2 1 5 1 0 2
Bud 6 3 6 1 13 7 16 21 21 22 15 25 4 3 3 15 11 8 4 5 2 3 7 2
Bud Neck 7 8 5 1 12 8 15 9 13 11 4 8 20 19 8 17 26 11 1 4 0 1 1 5
Bud Site 7 4 1 0 3 7 13 11 9 13 3 7 2 0 0 4 15 8 – – – – – –
Cell Periphery 1 4 0 1 0 0 0 0 0 0 0 1 0 0 0 0 2 2 0 0 0 0 1 0
Cytoplasm 153 165 105 121 82 70 86 69 44 65 32 53 233 191 222 104 92 77 111 136 66 90 119 153
Endoplasmic Reticulum 1 0 0 0 0 1 0 0 0 0 0 0 0 0 1 9 6 10 4 1 0 0 0 1
Endosome 0 12 3 3 6 6 2 8 3 2 5 1 1 4 4 19 8 17 11 7 6 5 7 7
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 1 1 1 1 1 0 0 0 1
Mitochondria 32 4 5 15 9 18 52 45 41 57 45 49 1 0 0 17 11 6 25 15 26 2 7 7
Nucleus 2 0 0 0 0 0 6 0 0 2 1 1 0 0 0 4 0 0 0 0 0 0 0 0
Nuclear Periphery 1 2 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Nucleolus 3 0 2 0 2 8 5 6 3 12 2 6 1 3 0 14 7 9 1 0 1 0 0 0
Peroxisomes 0 4 3 5 4 10 15 7 4 8 8 6 0 0 0 7 4 10 1 0 0 0 0 0
SpindlePole 35 35 15 11 52 57 91 95 68 72 47 43 71 64 58 169 138 115 45 59 17 31 48 58
Vac/Vac Membrane 0 1 4 0 2 0 2 2 3 1 2 1 0 0 0 4 4 1 6 2 0 1 4 8
Unique Cell Count 209 219 131 138 144 145 215 197 152 190 125 135 310 262 287 293 246 213 227 238 133 147 206 253
Labelled Cell Count 249 243 149 159 185 193 304 275 209 265 164 201 336 284 296 384 325 275 227 238 133 147 206 253


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 2.9 3.9 5.3 3.7 3.9 4.2 3.7 3.6 3.8 3.4 3.2 3.0 5.0 5.1 5.0 6.0 5.4 5.7 5.1 5.5 5.6
Std Deviation (1e-4) 0.7 0.8 3.9 1.9 1.6 3.3 2.4 1.8 2.2 1.8 1.5 0.8 0.9 1.0 1.1 2.3 1.5 2.0 1.2 1.4 1.6
Intensity Change (Log2) – – – -0.52 -0.43 -0.34 -0.49 -0.55 -0.47 -0.65 -0.7 -0.8 -0.07 -0.04 -0.08 0.19 0.03 0.12 -0.04 0.06 0.08


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –