Standard name
Human Ortholog
Description Putative ATPase of the CDC48/PAS1/SEC18 (AAA) family; localized to the cortex of mother cells but not to daughter cells; relocalizes from cytoplasm to plasma membrane foci upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0.12 0.11 0.05 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0.1 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0.1 0 0 0 – 0 0 0 0 0.05 0.05 0.08 0.06 0.1 0.08 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.98 0.99 0.99 0.97 0.95 – 0.82 0.81 0.75 0.8 0.79 0.74 0.88 0.95 0.95 0.86 0.89 0.93 0.84 0.82 0.79 0.83
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.09 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.06 0.12 – 0.25 0.29 0.42 0.27 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0.11 0.05 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0.1 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 2 1 0 0 0 – 2 1 2 2 35 23 19 1 3 0 1 1 5 2 6 9
Bud 0 3 1 1 0 1 0 – 7 9 8 7 29 7 7 0 2 0 0 0 2 0 3 7
Bud Neck 0 0 4 0 4 3 2 – 5 0 3 6 0 2 0 1 1 0 1 1 4 5 7 8
Bud Site 0 0 1 0 0 0 0 – 1 0 1 0 6 0 1 0 0 0 – – – – – –
Cell Periphery 0 6 9 18 11 8 14 – 5 4 10 12 16 11 29 11 18 8 0 2 10 6 8 3
Cytoplasm 46 194 260 180 253 229 346 – 207 207 228 268 234 161 330 184 164 90 65 191 227 155 261 269
Endoplasmic Reticulum 1 1 2 0 0 0 3 – 2 2 3 1 2 0 0 17 7 5 0 0 1 2 7 4
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 2 3 1 2 0 3 0 2 0 1 3 1
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 3 0 0 0 0 1 1
Mitochondria 1 0 0 2 2 14 43 – 62 73 129 90 3 3 0 4 0 2 0 2 7 6 12 6
Nucleus 0 0 2 0 1 0 2 – 1 4 1 1 0 0 0 1 2 0 0 0 1 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0
Nucleolus 0 0 0 0 0 0 1 – 0 1 1 0 0 0 0 0 1 2 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 1 11 23 19 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 3 0 1 1 1 – 4 1 6 5 9 21 10 0 1 0 0 0 1 0 1 1
Vac/Vac Membrane 0 0 1 0 0 0 1 – 0 1 1 0 1 0 0 3 2 4 2 0 1 3 8 5
Unique Cell Count 47 197 266 181 255 237 366 252 254 306 337 296 217 373 193 172 105 74 207 270 188 330 325
Labelled Cell Count 48 204 285 202 272 256 413 296 303 393 393 348 254 416 224 201 117 74 207 270 188 330 325


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 5.1 5.6 5.6 5.7 4.6 4.4 – 3.8 3.6 3.4 4.0 5.0 5.0 5.3 6.4 7.1 7.5 4.9 4.9 5.4
Std Deviation (1e-4) 0.5 0.8 0.8 2.0 1.5 1.8 1.3 – 1.3 1.2 0.5 1.0 0.9 0.7 1.2 1.0 1.2 1.6 0.8 1.1 1.6
Intensity Change (Log2) – – – 0.01 0.03 -0.26 -0.35 – -0.55 -0.63 -0.72 -0.47 -0.15 -0.15 -0.08 0.2 0.35 0.42 -0.19 -0.18 -0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 5.3 4.9 3.0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 5.0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 2.9 0.6 0 0 – 0 0 0 0 1.2 0.9 2.3 1.2 3.0 0
Cytoplasm 1.4 1.4 -0.8 -2.0 – -6.0 -6.1 -7.8 -6.7 -6.8 -7.7 -4.3 -1.4 -1.4 -4.5
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 4.3 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 3.2 5.4 3.7 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 1.6 4.3 1.4 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0