Standard name
Human Ortholog
Description Nucleolar protein required for 60S ribosome subunit biogenesis; constituent of 66S pre-ribosomal particles; physically interacts with Nop8p and the exosome subunit Rrp43p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0.05 0 – 0 0 0 0 0 0.08 0.07 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.05 0.09 0.18 – 0.12 0.14 0.14 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.06 0 0.11 0 – 0.06 0.06 0.08 0.12 0.12 0.11 0.15 0.07 0.13 0.08 0 0 0 0.08 0.08 0.07
Nucleus 0.46 0.57 0.5 0 0.08 0.06 0.08 – 0.11 0.12 0.12 0.12 0.13 0.08 0.05 0.18 0.14 0.15 0.23 0.35 0.22 0.08 0.06 0.07
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.53 0.49 0.47 0.95 0.86 0.73 0.84 – 0.82 0.83 0.81 0.77 0.89 0.84 0.87 0.8 0.84 0.84 0.64 0.57 0.65 0.68 0.66 0.64
Peroxisomes 0 0 0 0.06 0.06 0 0.11 – 0 0.07 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.07 0.07 0.13 0.17 – 0.07 0.13 0.08 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0.05 0.05 0 0 0.1 0 – 0 0 0 0 0 0 0 0.05 0 0.05 0.06 0.07 0.07 0.11 0.12 0.15
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0 2 3
Bud Neck 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud Site 0 0 0 0 0 2 0 – 1 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 1 6 3 11 20 12 – 9 6 4 5 2 21 19 2 1 5 0 0 0 1 0 2
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 6 2 0 0 0 1 0 0 0
Endosome 0 0 0 9 23 37 108 – 64 69 37 15 2 3 1 0 0 0 3 0 0 2 4 4
Golgi 0 0 0 0 4 8 0 – 0 1 0 0 0 0 0 1 0 0 0 0 1 1 0 2
Mitochondria 5 4 2 16 14 43 9 – 32 28 21 19 21 29 43 23 34 23 0 0 0 12 23 24
Nucleus 102 126 101 10 33 23 51 – 61 61 31 20 23 21 14 59 39 43 59 97 54 12 18 26
Nuclear Periphery 0 0 3 0 0 4 4 – 7 4 6 0 0 0 0 0 2 0 7 1 10 2 3 5
Nucleolus 118 109 95 252 364 291 512 – 444 422 212 126 159 223 244 257 227 246 162 160 164 112 202 229
Peroxisomes 6 1 0 16 24 6 66 – 23 33 11 2 4 4 13 2 1 1 0 0 0 0 0 1
SpindlePole 0 0 0 18 29 50 105 – 40 66 21 14 0 4 5 0 0 1 2 1 0 0 8 4
Vac/Vac Membrane 11 12 10 0 12 41 15 – 5 7 5 6 1 2 1 17 9 14 15 18 16 18 36 52
Unique Cell Count 224 223 204 265 425 396 606 542 506 262 163 179 266 281 322 271 293 255 282 252 165 306 362
Labelled Cell Count 245 253 217 324 514 525 882 687 697 348 207 212 307 341 367 315 333 255 282 252 165 306 362


Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 32.7 31.4 24.3 24.6 23.3 17.9 16.7 – 15.0 14.9 13.2 13.6 31.0 28.3 31.9 47.1 48.9 48.5 28.7 28.5 30.3
Std Deviation (1e-4) 9.3 9.4 6.7 9.8 8.7 11.9 4.9 – 4.3 5.2 3.9 3.8 13.1 12.0 17.2 19.4 16.0 16.6 7.4 9.5 10.3
Intensity Change (Log2) – – – 0.02 -0.06 -0.44 -0.54 – -0.69 -0.7 -0.88 -0.83 0.36 0.22 0.39 0.96 1.01 1.0 0.24 0.23 0.32


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 -0.3 1.2 0 – 0 0 0 0 0 2.3 1.9 0 0 0
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 3.4 4.5 6.5 – 5.1 5.6 5.6 4.4 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.8 1.7 4.4 0 – 2.9 2.7 3.5 4.4 4.4 4.3 5.4 3.2 4.7 3.4
Nucleus -11.6 -12.0 -12.5 -13.0 – -11.3 -10.8 -9.0 -7.5 -7.7 -10.2 -11.4 -7.6 -8.3 -8.4
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 11.9 10.3 6.5 10.8 – 9.6 10.0 7.8 6.0 8.7 8.6 9.5 7.9 8.6 8.8
Peroxisomes 3.6 3.5 0 4.9 – 3.0 3.7 0 0 0 0 3.1 0 0 0
SpindlePole 3.8 3.8 5.3 6.4 – 4.0 5.4 4.1 4.3 0 0 0 0 0 0
Vacuole 0 -1.3 2.3 -1.7 – 0 0 -1.8 -0.6 -2.5 -2.8 0 0.2 -0.9 -0.1