Standard name
Human Ortholog
Description GTPase-activating protein (GAP) for yeast Rab family members; involved in ER to Golgi trafficking; exhibits GAP activity toward Ypt1p that is stimulated by Gyl1p, also acts on Sec4p; interacts with Gyl1p, Rvs161p and Rvs167p; involved in recruiting Rvs167p to the bud tip during polarized growth; relocalizes from bud neck to cytoplasm upon DNA replication stress; GYP5 has a paralog, GYL1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.16 0.12 0 0.07 0.05 0.08 0 0 0 – 0 0 0 0 0 0.07 0.09 0.09 0.15 0.12 0.1 0.15 0.15 0.14
Bud 0.08 0.07 0 0 0.06 0.08 0.05 0.06 0 – 0.07 0.06 0 0 0 0 0 0 0.12 0.1 0 0.16 0.28 0.24
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.12 0.14 0.06 0.14 0.08 0.08
Bud Site 0.07 0.06 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0.05 0 0 0 0 0 – 0 0.05 0 0.05 0.05 0.05 0.09 0.09 0 0 0 0 0 0
Cytoplasm 0.73 0.66 0.85 0.81 0.83 0.76 0.8 0.78 0.76 – 0.67 0.68 0.92 0.99 0.97 0.72 0.74 0.78 0.4 0.4 0.5 0.24 0.24 0.32
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0.05 0.05 0 0 0 0 0 0
Endosome 0 0.07 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0.06 0 0.05 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.1 0.13 0.09 0.23 – 0.41 0.29 0 0 0 0 0 0 0 0.06 0.11 0.09 0.07 0.05
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0.1 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.05 0.08 0.08 0.1 0.12 0 0.05 0.08 0 – 0 0.05 0 0 0 0 0.05 0 0 0.05 0.07 0 0.06 0.06
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.13 0.07 0.1 0 0 0 0.05 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 40 35 10 14 13 22 17 15 9 – 5 5 6 0 1 22 18 21 44 41 27 25 68 57
Bud 19 19 3 8 14 23 19 23 6 – 19 15 7 2 6 5 0 2 35 34 10 27 125 94
Bud Neck 0 3 7 2 2 2 2 10 4 – 0 2 0 0 0 0 0 0 35 48 15 23 35 32
Bud Site 16 17 6 7 10 12 10 6 2 – 3 2 4 0 1 6 1 3 – – – – – –
Cell Periphery 3 7 8 10 8 1 4 6 11 – 8 11 13 19 20 17 18 22 1 3 3 1 3 4
Cytoplasm 178 192 212 173 201 213 309 291 224 – 173 165 308 368 428 224 153 189 117 132 133 40 108 127
Endoplasmic Reticulum 7 8 4 4 1 3 6 5 0 – 2 2 2 1 1 15 11 11 6 3 3 4 9 8
Endosome 3 19 0 2 1 1 4 2 4 – 0 2 7 3 4 11 6 3 17 18 7 7 13 11
Golgi 0 2 0 0 0 0 0 0 0 – 0 0 0 0 0 3 0 5 0 2 1 0 2 3
Mitochondria 6 9 0 2 2 29 49 33 69 – 106 69 5 1 0 11 3 4 10 20 29 15 31 20
Nucleus 1 0 1 1 3 1 4 6 2 – 2 2 0 0 2 4 1 3 2 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 1 0 0 1 – 2 0 0 0 0 0 0 0 1 0 0 0 0 0
Nucleolus 0 1 0 0 0 0 0 0 0 – 2 0 1 0 1 1 0 0 0 0 0 0 1 0
Peroxisomes 10 29 0 5 2 4 3 2 0 – 0 2 11 0 3 6 3 2 0 0 0 3 6 3
SpindlePole 12 22 21 21 28 11 20 30 6 – 6 12 8 1 2 13 10 8 8 15 18 6 26 22
Vac/Vac Membrane 0 5 3 0 2 1 3 7 8 – 3 4 1 0 2 40 14 23 3 7 6 8 12 9
Unique Cell Count 245 289 248 214 243 279 386 374 296 260 242 333 373 443 312 207 241 291 333 268 168 450 400
Labelled Cell Count 295 368 275 249 287 324 450 436 346 331 293 373 395 471 378 238 296 291 333 268 168 450 400


Ambiguous, Bud, Bud Neck, Cell Periphery, Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 5.9 6.5 5.8 6.5 4.9 5.5 5.6 5.0 – 4.4 5.1 5.8 6.0 6.1 9.5 10.3 9.7 5.4 5.4 5.9
Std Deviation (1e-4) 0.8 0.8 3.2 2.1 2.0 0.9 1.6 1.6 2.2 – 1.3 1.4 1.1 1.2 1.6 1.6 1.6 1.7 1.3 1.4 1.7
Intensity Change (Log2) – – – -0.18 -0.0 -0.42 -0.25 -0.22 -0.39 – -0.56 -0.35 -0.18 -0.12 -0.09 0.53 0.65 0.57 -0.28 -0.27 -0.15


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.2 0.7 1.8 0.2 0 -0.6 – 0 -1.3 -1.6 0 0 1.5 2.1 2.1
Bud 1.8 2.8 3.7 2.5 3.0 0 – 0 2.9 0 0 0 0 0 0
Bud Neck 0 0 0 0 -0.1 -1.2 – 0 0 0 0 0 0 0 0
Bud Site 0.6 1.1 1.2 0.1 0 0 – 0 0 0 0 0 -0.4 0 0
Cell Periphery 0.8 0 0 0 -1.3 0.3 – 0 0.8 0.4 1.1 0.8 1.3 2.5 2.7
Cytoplasm -1.3 -0.8 -2.7 -1.7 -2.4 -2.9 – -5.0 -4.5 2.7 6.5 5.4 -3.9 -3.1 -2.0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 2.1 2.2 1.9
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 5.2 5.8 4.8 8.1 – 0 9.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0.5 1.1 -2.2 -1.6 -0.2 -3.4 – 0 -1.5 -3.3 -5.4 -5.6 -2.1 -1.5 -2.4
Vacuole 0 0 0 0 0 1.2 – 0 0 0 0 0 5.1 3.1 4.1