Standard name
Human Ortholog
Description Protein of unknown function; component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0.05 – 0 0.05 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0 0 0.07 0.06 0 – 0 0 0.05 0 0.09 0.16 0.19 0.09 0.12 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.34 0 0 0.15 0 0.09 0.15 – 0.32 0.28 0.57 0.33 0 0 0 0.07 0.06 0 0 0 0 0 0 0
Nucleus 0.88 0.89 0.93 0.93 0.86 0.87 0.89 – 0.81 0.87 0.73 0.77 0.9 0.82 0.8 0.8 0.77 0.79 0.77 0.72 0.78 0.76 0.7 0.67
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.09 0 0 0 0 0 0 – 0 0 0.07 0.05 0 0 0 0.05 0 0.06 0.1 0.1 0.09 0.09 0.11 0.13
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.05 0.05 0 0 0.06 – 0 0.05 0 0 0.05 0 0.05 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.06 0.07 0.07 0.06 0 0.08 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0
Bud 0 0 0 1 0 0 0 – 0 3 1 3 0 0 0 0 0 0 0 5 1 0 1 3
Bud Neck 0 0 2 0 0 1 1 – 1 2 1 0 1 0 0 0 0 0 0 0 1 0 0 0
Bud Site 0 0 0 0 1 0 8 – 4 9 11 12 0 0 0 0 0 1 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 1 15 4 3 10 10 6 – 5 6 14 11 17 35 53 16 23 11 0 1 0 4 4 4
Endoplasmic Reticulum 0 0 0 0 1 0 0 – 1 0 0 0 1 1 0 5 10 4 0 0 0 0 0 1
Endosome 0 5 1 0 4 0 0 – 0 1 0 1 0 0 0 4 8 2 1 5 1 0 3 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 3 2 1 0 1 0 0 0 1
Mitochondria 47 6 1 12 4 14 25 – 38 55 166 93 1 0 0 14 11 5 1 3 0 1 4 3
Nucleus 123 270 94 75 131 138 152 – 96 169 214 215 167 185 218 150 149 106 113 245 94 119 167 154
Nuclear Periphery 1 0 0 0 0 1 0 – 0 2 0 1 0 0 1 0 1 0 0 0 0 0 1 1
Nucleolus 12 13 2 1 3 3 2 – 2 6 20 15 1 4 3 9 7 8 15 34 11 14 26 28
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 2 6 5 4 5 0 11 – 3 9 2 5 9 10 15 5 1 4 2 8 2 6 7 7
Vac/Vac Membrane 0 10 2 2 1 5 1 – 1 1 0 5 2 1 1 5 7 8 10 25 7 5 18 17
Unique Cell Count 139 304 101 81 153 158 170 119 195 293 281 185 225 273 188 194 135 147 339 121 156 239 231
Labelled Cell Count 186 325 111 98 160 172 206 151 263 429 361 200 236 292 211 219 150 147 339 121 156 239 231


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 6.1 5.9 4.9 6.4 5.2 5.0 – 4.7 4.5 4.2 4.5 6.3 6.2 6.0 7.9 8.3 8.2 5.7 5.9 6.2
Std Deviation (1e-4) 0.6 1.1 0.7 0.6 1.9 1.5 0.9 – 1.4 0.9 1.7 1.0 1.1 1.2 1.1 2.0 2.2 2.2 1.1 1.4 1.3
Intensity Change (Log2) – – – -0.27 0.11 -0.2 -0.25 – -0.34 -0.38 -0.5 -0.4 0.08 0.07 0.01 0.41 0.48 0.46 -0.06 0.0 0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.9 0.8 0 – 0 0 0 0 1.6 3.0 3.7 1.5 2.2 1.3
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.6 0 2.6 3.7 – 0 0 0 0 0 0 0 2.4 0 0
Nucleus -0.1 -1.8 -1.5 -1.0 – -2.7 -1.7 -4.2 -3.6 -0.8 -2.6 -3.1 -3.0 -3.5 -3.1
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.5 – 0 0 0 0 0 0 0.2 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.5247 -0.0417 -0.1036 -0.5705 -0.7978 -0.2347 0.262 0.4219 0.4973 0.0932 -0.1931 0.5062 0.6855 0.5059 0.4268 0.6898 0.0841 0.6579
Actin 0.0127 0.0001 0.0098 0 0.001 0.0007 0.0106 0.0001 0.0061 0.017 0.002 0.0002 0.0164 0 0.0244 0.0107 0 0.0036
Bud 0.0002 0 0.0007 0 0.0003 0 0.0002 0 0.0001 0.0003 0.0001 0.0002 0.0002 0.0001 0.0006 0.0001 0 0.0004
Bud Neck 0.0006 0.0002 0.0002 0 0.0013 0.0014 0.002 0.0001 0.0002 0.0001 0.0008 0.002 0.0035 0.0002 0.0007 0.0004 0.0007 0.0013
Bud Periphery 0.0007 0 0.0013 0 0.0023 0 0.0006 0 0.0003 0.0003 0.0002 0.0009 0.0002 0.0001 0.001 0.0001 0.0001 0.0009
Bud Site 0.0016 0.0002 0.002 0 0.0008 0.0001 0.0007 0.0001 0.0008 0.0028 0.0004 0.0003 0.0043 0.0002 0.0018 0.0003 0.0001 0.0013
Cell Periphery 0.0002 0 0.0001 0 0.0008 0 0.0001 0 0 0 0.0001 0.0001 0.0002 0 0.0001 0 0 0.0001
Cytoplasm 0.0085 0.0005 0.0014 0.0001 0.0002 0.0002 0.0021 0.0012 0.0011 0.0013 0.0028 0.0013 0.0027 0.0012 0.0005 0.0041 0.0001 0.0007
Cytoplasmic Foci 0.0068 0.0001 0.0037 0 0.0004 0.0017 0.0108 0.0003 0.0118 0.0073 0.0102 0.0073 0.0073 0 0.0039 0.0027 0 0.0032
Eisosomes 0.0001 0 0.0001 0 0.0001 0 0.0002 0 0.0001 0.0001 0.0001 0 0.0002 0 0.0001 0.0001 0 0.0001
Endoplasmic Reticulum 0.0008 0.0002 0.0009 0 0.0006 0 0.001 0.0001 0.0002 0.0015 0.0009 0.0002 0.0007 0.0001 0.0001 0.0006 0 0.0005
Endosome 0.0199 0.0001 0.0086 0 0.0147 0.0006 0.0094 0.0002 0.0073 0.011 0.0091 0.0083 0.0045 0.0001 0.0049 0.0057 0 0.0061
Golgi 0.0028 0 0.0024 0 0.0026 0.0003 0.0036 0 0.0035 0.0043 0.0101 0.0009 0.0031 0 0.0056 0.0067 0 0.0046
Lipid Particles 0.0199 0 0.0034 0 0.0062 0.0012 0.0118 0.0001 0.0236 0.0042 0.0573 0.0016 0.0084 0 0.0051 0.0186 0 0.0165
Mitochondria 0.013 0.0001 0.0152 0.0001 0.1513 0.0001 0.0162 0.0003 0.0164 0.002 0.0194 0.0024 0.0027 0.0002 0.0035 0.002 0.0004 0.022
None 0.0225 0.0163 0.0009 0 0.0003 0.0001 0.0242 0.0047 0.0049 0.0016 0.0016 0.0025 0.0176 0.0001 0.0002 0.0246 0 0.0044
Nuclear Periphery 0.0064 0.0056 0.0089 0.0004 0.0038 0.0008 0.023 0.0027 0.0021 0.0035 0.0111 0.0109 0.007 0.0037 0.002 0.0061 0.0007 0.006
Nucleolus 0.0217 0.0263 0.0164 0.0054 0.0664 0.0564 0.0142 0.0179 0.0167 0.0053 0.0363 0.0383 0.013 0.0227 0.0114 0.0086 0.046 0.0389
Nucleus 0.8001 0.9473 0.9085 0.9416 0.6849 0.9214 0.8185 0.9651 0.8588 0.9274 0.7414 0.9019 0.8808 0.9582 0.9139 0.8949 0.9481 0.8658
Peroxisomes 0.0053 0 0.0108 0 0.0009 0.0012 0.0283 0 0.0218 0.0042 0.025 0.0004 0.0055 0 0.0029 0.0027 0 0.0079
Punctate Nuclear 0.0495 0.0026 0.0016 0.0523 0.0013 0.0132 0.0191 0.0067 0.0226 0.0051 0.0674 0.0184 0.0195 0.013 0.0166 0.0105 0.0032 0.0138
Vacuole 0.0051 0.0002 0.0018 0 0.034 0.0005 0.0014 0.0001 0.0007 0.0003 0.0028 0.001 0.0015 0.0001 0.0004 0.0002 0.0002 0.001
Vacuole Periphery 0.0015 0.0001 0.0014 0 0.0257 0 0.002 0.0001 0.001 0.0003 0.0009 0.0007 0.0006 0 0.0002 0.0003 0.0001 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.4007 9.4541 6.2359 8.1554 8.7604 9.3955 10.8867 11.4697 13.1903 10.0894
Translational Efficiency 0.7566 0.9515 1.3414 1.1082 0.7716 0.7313 0.7395 0.6522 0.5373 0.6973

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein of unknown function; component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae
Localization
Cell Percentages nucleus (90%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Swc3

Swc3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Swc3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available