Standard name
Human Ortholog
Description Cytochrome c heme lyase (holocytochrome c synthase); attaches heme to apo-cytochrome c (Cyc1p or Cyc7p) in mitochondrial intermembrane space; human homolog HCCS implicated in microphthalmia with linear skin defects (MLS), and can complement yeast null mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.1 0 0 0 0 0 0 – 0 0 0 0.12 0.07 0.09 0.11 0.14 0.05 0.07 0.07 0.06 0.06 0.08 0.08
Bud 0 0 0.07 0 0 0 0.06 0 – 0 0.06 0.07 0 0 0 0 0 0 0 0 0 0 0.05 0.08
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.09 0 0 0 0 0 0 – 0 0 0 0.06 0 0 0.11 0.09 0.1 0 0 0 0 0 0
Cytoplasm 0.16 0.63 0.44 0.54 0.18 0.08 0.08 0.06 – 0.07 0 0.05 0.46 0.47 0.51 0.41 0.44 0.41 0.12 0.19 0.15 0.14 0.11 0.1
Endoplasmic Reticulum 0.21 0.14 0.09 0.07 0 0.07 0 0 – 0 0 0 0.28 0.21 0.15 0 0.05 0.07 0.11 0.08 0.07 0.1 0.1 0.05
Endosome 0 0 0.06 0 0.2 0 0.05 0 – 0 0 0 0 0.06 0.06 0.1 0.07 0.06 0 0.1 0.09 0.09 0.1 0.16
Golgi 0.14 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.1 0.08 0.1 0.14 0 0 0 0.05 0.1
Mitochondria 0.53 0.05 0.41 0.46 0.59 0.83 0.82 0.85 – 0.85 0.93 0.93 0 0 0 0.06 0 0.09 0.36 0.3 0.51 0.33 0.24 0.17
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.07 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.12 0.06 0.08 0 0.2 0.12 0.19 0.12 – 0.14 0.09 0.08 0.14 0.21 0.22 0.23 0.21 0.3 0 0.11 0 0.13 0.17 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 10 7 4 4 8 0 0 – 1 2 1 19 16 21 19 24 8 5 12 13 10 19 26
Bud 0 3 13 1 11 9 30 19 – 21 26 40 1 1 3 0 2 3 3 3 1 4 11 26
Bud Neck 0 0 0 0 0 0 1 0 – 1 0 1 0 0 0 0 0 0 0 7 3 7 10 8
Bud Site 0 0 1 0 1 10 6 7 – 7 2 6 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 9 2 2 6 1 3 1 – 6 6 4 9 5 8 19 16 14 0 1 1 2 2 1
Cytoplasm 7 61 82 75 65 31 45 39 – 37 14 32 75 100 118 73 77 61 9 32 34 26 28 34
Endoplasmic Reticulum 9 14 17 10 7 30 1 4 – 8 6 6 45 45 34 5 8 10 8 13 16 19 24 16
Endosome 1 4 12 4 74 2 29 18 – 13 1 6 6 13 13 18 13 9 3 16 20 16 24 51
Golgi 6 1 0 0 2 1 1 0 – 0 2 1 0 8 7 18 14 15 10 3 2 7 13 34
Mitochondria 23 5 77 65 214 331 439 589 – 483 378 567 1 8 4 11 7 13 28 49 119 62 61 55
Nucleus 0 0 0 0 2 12 9 13 – 6 3 5 1 2 3 2 3 1 0 1 0 0 2 2
Nuclear Periphery 0 0 0 0 1 2 8 8 – 12 9 4 0 0 1 0 0 0 0 1 0 0 2 4
Nucleolus 3 0 0 2 5 4 13 21 – 12 9 6 0 0 0 1 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 2 1 – 2 1 0 0 0 0 0 0 0 0 0 1 0 0 1
SpindlePole 0 0 2 0 6 3 13 14 – 18 5 9 2 1 0 2 2 1 1 1 4 1 4 13
Vac/Vac Membrane 5 6 15 6 73 49 103 83 – 78 37 52 23 45 50 42 37 44 3 18 7 24 42 41
Unique Cell Count 43 97 186 140 364 401 537 690 566 408 612 163 215 230 180 175 147 80 166 234 188 253 329
Labelled Cell Count 55 113 228 169 472 493 703 817 705 501 740 182 244 262 210 203 180 80 166 234 188 253 329


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.7 7.1 4.9 5.2 5.9 4.9 4.5 4.4 – 4.5 4.3 4.5 8.3 8.1 8.0 9.6 9.8 10.4 9.0 9.6 10.5
Std Deviation (1e-4) 2.7 0.9 0.8 1.0 1.3 0.8 1.0 0.9 – 0.9 0.9 0.9 1.1 1.0 1.1 1.6 1.7 2.0 1.5 1.9 2.3
Intensity Change (Log2) – – – 0.09 0.26 0.02 -0.13 -0.14 – -0.11 -0.19 -0.12 0.77 0.73 0.72 0.98 1.0 1.09 0.88 0.98 1.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 2.8 1.6 2.2 2.5 3.4 0.7
Bud -2.8 -2.2 -2.8 0 0 – 0 0 0 -3.0 -3.5 -3.0 -3.6 -2.8 -2.1
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 2.4 0 0 3.9 3.5 3.6
Cytoplasm 1.7 -6.6 -10.4 -11.0 -13.5 – -12.2 -12.5 -13.3 0.4 0.5 1.5 -0.7 0 -0.5
Endoplasmic Reticulum -0.6 -3.9 -0.7 0 0 – 0 0 0 4.5 3.3 1.7 -2.6 -1.7 -0.8
Endosome -1.5 4.2 0 0 0 – 0 0 0 -1.2 -0.2 -0.3 1.2 0.4 -0.1
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 4.4 3.9 4.5
Mitochondria 0.9 3.9 10.1 0 0 – 0 0 0 -9.1 -9.2 -10.2 -7.9 -8.4 -6.6
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole -1.4 3.6 1.5 3.5 1.5 – 2.1 0.4 0.2 1.8 3.6 3.8 4.0 3.5 5.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.6716 4.7763 4.1438 3.6913 3.3166 4.3093 5.6029 6.591 6.035 5.8547 5.1877 6.26 4.5631 5.1598 4.9637 4.0565 4.1315 4.7818
Actin 0.0202 0.0151 0.0287 0.0427 0.0273 0.0183 0.0495 0.0175 0.0374 0.0659 0.0384 0.0313 0.0269 0.0156 0.0332 0.0198 0.0185 0.0194
Bud 0.0026 0.0046 0.012 0.0081 0.0087 0.0031 0.0035 0.0033 0.0081 0.0197 0.0096 0.0033 0.0036 0.0062 0.0085 0.0197 0.0098 0.0052
Bud Neck 0.0042 0.0021 0.0032 0.0032 0.0091 0.0099 0.0027 0.0028 0.007 0.003 0.0026 0.0158 0.0045 0.0044 0.0039 0.0037 0.0058 0.0138
Bud Periphery 0.003 0.0029 0.0163 0.0184 0.0134 0.0035 0.0042 0.0023 0.0114 0.0489 0.0093 0.0045 0.0048 0.005 0.0087 0.0274 0.0131 0.0081
Bud Site 0.0142 0.0526 0.0258 0.0041 0.0068 0.0039 0.0217 0.0388 0.0335 0.0079 0.0071 0.003 0.0201 0.0499 0.0262 0.0136 0.0064 0.0033
Cell Periphery 0.0021 0.0019 0.0056 0.0092 0.0014 0.0009 0.0041 0.0033 0.0071 0.0031 0.0013 0.0023 0.0041 0.0029 0.0048 0.0018 0.0029 0.0015
Cytoplasm 0.4055 0.5184 0.4807 0.5762 0.4202 0.4676 0.3132 0.5629 0.4477 0.4751 0.3769 0.4032 0.278 0.5326 0.421 0.4486 0.4474 0.3916
Cytoplasmic Foci 0.1234 0.1131 0.1057 0.0632 0.1561 0.1212 0.1018 0.0964 0.0972 0.0678 0.1103 0.1094 0.1162 0.0806 0.1092 0.1009 0.1154 0.1112
Eisosomes 0.0005 0.0002 0.0007 0.0004 0.0003 0.0001 0.0009 0.0002 0.001 0.0005 0.0003 0.0003 0.0005 0.0002 0.0005 0.0002 0.0001 0.0002
Endoplasmic Reticulum 0.0289 0.0299 0.0276 0.0178 0.0054 0.0342 0.0803 0.0293 0.0367 0.0217 0.032 0.0303 0.0817 0.04 0.037 0.0297 0.02 0.0386
Endosome 0.2015 0.1592 0.133 0.1144 0.1472 0.1845 0.1671 0.1074 0.1073 0.0822 0.1437 0.1488 0.2479 0.1377 0.1485 0.1179 0.1167 0.1974
Golgi 0.0594 0.0566 0.0583 0.0436 0.0772 0.0629 0.0915 0.0695 0.0921 0.0647 0.1206 0.0873 0.0802 0.0589 0.0889 0.063 0.0939 0.0929
Lipid Particles 0.0092 0.0013 0.0038 0.0017 0.003 0.0037 0.0181 0.0013 0.0028 0.002 0.0096 0.0052 0.0117 0.0044 0.0063 0.0083 0.0049 0.004
Mitochondria 0.0444 0.0108 0.0535 0.0679 0.0585 0.0485 0.0674 0.0427 0.0851 0.1201 0.1005 0.1155 0.052 0.0369 0.0756 0.0908 0.0949 0.0823
None 0.0321 0.0127 0.0211 0.0078 0.0328 0.0104 0.0298 0.0073 0.0029 0.0037 0.0019 0.0165 0.0102 0.003 0.0034 0.0042 0.0031 0.0045
Nuclear Periphery 0.0051 0.0029 0.0025 0.0026 0.0011 0.003 0.0081 0.0016 0.0016 0.0012 0.0018 0.0011 0.0121 0.0025 0.0022 0.0023 0.0013 0.0036
Nucleolus 0.0005 0.0002 0.0004 0.0006 0.0025 0.0002 0.0004 0.0002 0.0002 0.0001 0.0001 0.0001 0.0008 0.0002 0.0003 0.0013 0.0002 0.0002
Nucleus 0.0029 0.0024 0.0023 0.0035 0.0038 0.0024 0.003 0.0017 0.0014 0.0012 0.001 0.0013 0.0053 0.0024 0.0022 0.0028 0.0018 0.0028
Peroxisomes 0.0157 0.0035 0.0079 0.0042 0.0114 0.0085 0.015 0.0039 0.0084 0.0034 0.0222 0.011 0.0083 0.0029 0.0075 0.0255 0.031 0.0061
Punctate Nuclear 0.0042 0.0007 0.0018 0.0006 0.0054 0.0015 0.0041 0.0006 0.0006 0.0004 0.0005 0.0006 0.0042 0.0007 0.001 0.0036 0.0014 0.0017
Vacuole 0.0167 0.007 0.0071 0.0066 0.0066 0.0083 0.0106 0.0053 0.0084 0.0057 0.0061 0.0065 0.0219 0.0108 0.0082 0.0109 0.008 0.008
Vacuole Periphery 0.0039 0.0019 0.0022 0.0033 0.0017 0.0033 0.0032 0.0016 0.002 0.0018 0.0041 0.0027 0.0051 0.0021 0.003 0.0037 0.0033 0.0035

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.3467 34.097 31.6268 24.0485 15.0126 32.6754 21.2393 30.3322 43.5955 21.3647
Translational Efficiency 2.2908 1.3117 1.5109 2.3992 2.0289 1.3433 2.4583 2.309 1.5641 1.7537

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Cytochrome c heme lyase (holocytochrome c synthase); attaches heme to apo-cytochrome c (Cyc1p or Cyc7p) in mitochondrial intermembrane space; human homolog HCCS implicated in microphthalmia with linear skin defects (MLS), and can complement yeast null mutant
Localization
Cell Percentages mitochondrion (45%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Cyc3

Cyc3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cyc3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available