Standard name
Human Ortholog
Description NAD-dependent (R,R)-butanediol dehydrogenase; catalyzes oxidation of (R,R)-2,3-butanediol to (3R)-acetoin, oxidation of meso-butanediol to (3S)-acetoin, and reduction of acetoin; enhances use of 2,3-butanediol as an aerobic carbon source
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.99 0.99 1.0 0.99 0.97 0.89 0.85 0.85 0.87 0.93 0.85 1.0 0.99 0.99 0.99 1.0 0.97 0.98 0.97 0.96 0.97 0.92 0.96
Endoplasmic Reticulum 0 0 0 0 0 0 0.08 0.12 0.1 0.09 0 0.1 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 2 0 4 5 3 5 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 4 1 4 2 3 2 0 0 0 0 0 0 0 0 1 1 3 6
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 5 6 5 3 10 26 17 15 27 14 28 0 1 4 0 0 0 0 0 0 0 0 0
Cytoplasm 145 163 396 262 436 468 574 472 350 462 329 445 290 444 448 125 134 114 140 156 374 226 300 386
Endoplasmic Reticulum 0 0 0 0 7 11 50 67 39 49 12 51 1 0 4 4 8 5 0 1 2 0 1 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Golgi 0 0 0 0 0 0 0 1 2 1 2 4 0 0 1 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 1 0 3 4 2 2 0 0 0 0 0 2 0 0 1 0 0 0
Nucleus 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0 0 1 0 1 2 1 8 3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0
Nucleolus 0 0 0 0 0 0 0 0 3 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 2 1 0 0 3 5 3 4 7 7 0 4 0 0 0 1 0 0 1 0 4 3
Unique Cell Count 145 164 398 263 441 483 642 553 410 532 355 524 290 447 451 126 134 117 143 162 389 234 325 404
Labelled Cell Count 146 168 404 268 446 489 660 565 424 555 372 544 291 449 457 129 142 125 143 162 389 234 325 404


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 48.2 60.6 53.4 48.6 52.8 49.3 45.2 48.4 44.8 44.5 42.4 42.3 65.2 71.7 77.2 73.2 78.5 77.7 47.8 46.9 49.1
Std Deviation (1e-4) 9.6 11.0 9.8 8.6 10.9 10.2 10.7 12.6 12.5 11.7 12.3 12.0 14.3 19.2 16.5 28.7 22.9 29.5 11.5 12.3 12.6
Intensity Change (Log2) – – – -0.14 -0.01 -0.11 -0.24 -0.14 -0.25 -0.26 -0.33 -0.33 0.29 0.43 0.53 0.46 0.56 0.54 -0.16 -0.19 -0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0.6 2.3 1.6 1.9 2.9 2.1 3.1 0 0 0 0 0 0
Cytoplasm 0.2 -1.0 -2.8 -6.3 -7.6 -7.5 -7.1 -4.9 -7.7 1.2 -0.3 -0.3 -0.4 0.8 -2.0
Endoplasmic Reticulum 0 0 0 5.7 7.2 6.3 6.2 3.7 6.4 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 38.5499 43.7819 43.3443 36.9064 38.3404 42.1549 43.1319 54.5825 52.6876 46.0354 47.4422 50.7035 40.0072 48.5523 45.0407 44.19 37.1262 47.0463
Actin 0.0001 0.0001 0.0001 0.0002 0.0001 0.0002 0.0048 0.0002 0.0006 0.0215 0.0001 0.0005 0.0175 0.0001 0.0118 0 0.0003 0.0014
Bud 0.0001 0 0 0.0002 0 0.0001 0.0014 0 0.0002 0.0009 0.0005 0.0001 0.002 0.0001 0.0016 0 0.0009 0.0001
Bud Neck 0.0001 0.0001 0.0002 0.0003 0.0003 0.0005 0.0006 0.0001 0.0001 0.0005 0.0001 0.0004 0.0005 0.0001 0.0012 0.0002 0.0003 0.0002
Bud Periphery 0 0 0 0.0001 0 0 0.0013 0 0.0002 0.001 0.0002 0 0.0008 0 0.0007 0 0.0002 0.0001
Bud Site 0.0001 0 0 0.0002 0 0 0.0007 0 0.0002 0.0011 0.0001 0.0001 0.0007 0 0.0036 0 0.0001 0.0001
Cell Periphery 0.0001 0 0 0.0002 0 0 0.0011 0 0.0005 0.0004 0.0001 0 0.0001 0 0.0001 0 0.0001 0.0001
Cytoplasm 0.9721 0.9947 0.9911 0.9592 0.9869 0.9748 0.8767 0.9903 0.9588 0.8593 0.9793 0.9702 0.9061 0.9964 0.967 0.9939 0.9679 0.9433
Cytoplasmic Foci 0.0009 0.0002 0.0002 0.0011 0.0003 0.0027 0.0119 0.0001 0.0022 0.0165 0.0026 0.0025 0.0093 0.0002 0.0031 0.0001 0.01 0.0015
Eisosomes 0 0 0 0 0 0 0.0002 0 0.0001 0.0001 0 0 0.0001 0 0 0 0 0.0001
Endoplasmic Reticulum 0.0005 0.0002 0.0002 0.002 0.0003 0.0007 0.0038 0.0001 0.0012 0.0018 0.0005 0.0009 0.0032 0.0001 0.0002 0 0.0009 0.0003
Endosome 0.0005 0 0 0.0011 0.0001 0.0007 0.0072 0 0.0019 0.0092 0.0009 0.0059 0.0136 0 0.0007 0 0.0053 0.0096
Golgi 0 0 0 0 0 0 0.0013 0 0.0005 0.0086 0.0001 0.0006 0.0062 0 0.0011 0 0.0002 0.0135
Lipid Particles 0.0001 0 0 0.0003 0 0.0001 0.0048 0 0.0036 0.0123 0.0002 0.0007 0.0043 0 0.0003 0 0.0005 0.0008
Mitochondria 0.0003 0 0 0.0005 0 0.0002 0.0122 0.0056 0.0105 0.0107 0.0006 0.0008 0.0024 0 0.0009 0 0.0002 0.0236
None 0.0007 0.0001 0.0001 0.0022 0.0001 0.0008 0.007 0.0001 0.0019 0.013 0.0014 0.0016 0.003 0.0002 0.0006 0 0.0009 0.0002
Nuclear Periphery 0.0035 0.0007 0.0015 0.0062 0.0017 0.0023 0.0124 0.0004 0.0036 0.0024 0.0015 0.0025 0.0119 0.0003 0.0005 0.0005 0.0013 0.0005
Nucleolus 0.0012 0 0 0.0004 0 0.002 0.0016 0 0.0007 0.0089 0.0003 0.0003 0.0003 0 0.0004 0 0.0001 0.0001
Nucleus 0.0164 0.0035 0.0064 0.0206 0.0096 0.0118 0.0311 0.0026 0.0063 0.0109 0.006 0.0098 0.0122 0.0021 0.005 0.0052 0.004 0.0034
Peroxisomes 0 0 0 0 0 0 0.0021 0 0.0011 0.0098 0.0001 0.0001 0.0011 0 0.0007 0 0.0001 0.0003
Punctate Nuclear 0.0004 0.0001 0.0001 0.0006 0.0001 0.0012 0.0024 0.0001 0.0006 0.0032 0.0002 0.0007 0.0018 0.0001 0.0003 0 0.0009 0.0001
Vacuole 0.0019 0.0001 0.0001 0.004 0.0004 0.0012 0.0115 0.0001 0.0032 0.0038 0.0039 0.0017 0.002 0.0001 0.0005 0 0.0049 0.0003
Vacuole Periphery 0.0009 0 0 0.0007 0.0001 0.0004 0.0041 0 0.002 0.0042 0.0015 0.0006 0.0009 0 0.0001 0 0.0009 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 54.2579 30.0463 36.947 56.899 41.0881 94.059 87.4503 103.4002 154.43 97.1071
Translational Efficiency 0.9751 1.0299 0.9819 1.1313 0.875 0.9535 1.0504 1.1453 1.037 1.0588

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description NAD-dependent (R,R)-butanediol dehydrogenase; catalyzes oxidation of (R,R)-2,3-butanediol to (3R)-acetoin, oxidation of meso-butanediol to (3S)-acetoin, and reduction of acetoin; enhances use of 2,3-butanediol as an aerobic carbon source
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Bdh1

Bdh1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bdh1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available