Standard name
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein colocalizes with clathrin-coated vesicles
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0.1 0.06 0 0 0.05 0.09 0.08 0.05 0.08 0 0.07 0.08
Bud 0 0.06 0 0.06 0.06 0.05 0.07 0.07 0.09 0.1 0.1 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.59 0.68 0.66 0.51 0.31 0.21 0.24 0.31 0.17 0.23 0.18 0.25 0.65 0.59 0.56 0.59 0.48 0.48 0.23 0.36 0.24 0.45 0.33 0.26
Endoplasmic Reticulum 0.11 0.06 0 0 0 0 0 0 0 0 0 0 0.11 0 0 0 0.05 0.05 0 0 0 0 0 0
Endosome 0.1 0 0.11 0.19 0.23 0.22 0.21 0.17 0 0.05 0 0.05 0.08 0.22 0.29 0.22 0.31 0.22 0.22 0.07 0.13 0.1 0.13 0.16
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.14 0.28 0 0.18 0.17 0.43 0.37 0.25 0.76 0.66 0.74 0.57 0.05 0 0 0.09 0.1 0.09 0.25 0.31 0.33 0.25 0.25 0.26
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0.06 0.12 0.07 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.15 0.1 0.19 0.18 0.2 0.22 0.1 0.14 0.11 0.15 0.07 0.1 0.12 0.12 0.1 0.14 0 0 0 0 0.06 0.06
Vac/Vac Membrane 0 0 0.07 0.05 0.11 0.06 0.07 0.12 0 0 0 0 0 0 0 0.06 0.06 0.09 0 0.06 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 8 8 4 10 6 3 3 2 5 2 10 5 15 19 12 1 13 26 15 10 25 10 24 30
Bud 5 8 6 15 22 21 41 28 38 44 37 51 5 12 3 6 10 9 2 1 11 4 7 8
Bud Neck 0 0 1 2 7 1 5 2 1 1 5 1 1 0 1 0 0 0 5 7 8 4 11 9
Bud Site 0 1 0 0 0 9 6 0 8 7 6 8 0 0 0 1 0 1 – – – – – –
Cell Periphery 1 1 2 4 3 3 3 4 1 0 0 3 1 0 2 4 4 1 0 0 1 0 0 0
Cytoplasm 78 96 154 126 110 94 141 128 76 103 69 120 97 200 202 127 136 133 45 66 81 120 117 99
Endoplasmic Reticulum 14 8 1 0 4 5 0 1 5 0 3 3 16 11 14 6 14 13 6 0 6 2 4 6
Endosome 13 1 26 47 82 102 124 71 13 21 9 26 12 74 104 47 87 60 44 13 45 26 45 62
Golgi 1 0 0 1 0 0 1 1 0 0 0 0 1 0 2 0 2 6 7 3 5 4 6 12
Mitochondria 18 40 10 45 62 194 215 105 339 289 285 270 7 12 1 19 27 26 50 57 115 66 88 101
Nucleus 1 0 0 0 1 3 3 11 2 5 1 6 0 1 1 1 2 8 0 1 2 1 4 2
Nuclear Periphery 0 0 0 0 0 0 0 1 0 1 0 1 1 0 0 0 0 0 0 0 1 0 1 1
Nucleolus 0 0 1 0 2 3 4 2 5 2 4 7 0 0 0 0 2 1 0 0 0 1 1 0
Peroxisomes 0 0 0 14 44 30 24 22 11 9 9 21 0 12 12 3 5 1 2 1 6 0 3 4
SpindlePole 3 1 35 26 69 81 117 91 43 60 42 73 10 33 45 27 28 38 7 5 14 7 22 24
Vac/Vac Membrane 4 1 16 12 40 26 40 49 12 8 8 13 0 5 11 12 16 25 3 11 4 11 9 11
Unique Cell Count 132 141 234 249 356 454 579 414 447 440 384 477 149 337 362 217 281 276 197 185 344 271 360 385
Labelled Cell Count 146 165 256 302 452 575 727 518 559 552 488 608 166 379 410 254 346 348 197 185 344 271 360 385


Late Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 4.2 6.6 5.9 6.4 5.1 5.2 5.8 4.3 4.6 4.2 4.7 5.3 5.7 5.8 5.5 6.5 6.7 6.9 6.9 6.9
Std Deviation (1e-4) 0.7 0.6 2.2 1.6 2.1 1.2 1.7 1.7 0.9 0.9 0.9 1.1 0.9 0.9 1.3 1.2 1.3 1.2 2.0 2.0 1.9
Intensity Change (Log2) – – – -0.16 -0.05 -0.38 -0.34 -0.18 -0.62 -0.53 -0.63 -0.49 -0.31 -0.2 -0.18 -0.26 -0.01 0.02 0.06 0.06 0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – -0.6 -0.5 0.9