Standard name
Human Ortholog
Description Methionyl-tRNA formyltransferase; catalyzes the formylation of initiator Met-tRNA in mitochondria; potential Cdc28p substrate
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud 0 0 0 0 0.05 0 0.05 0.06 0 0.07 0.08 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Cytoplasm 0.52 0.88 0.76 0.75 0.61 0.36 0.21 0.18 0.17 0.11 0.1 0.09 0.95 0.97 0.96 0.65 – – 0.23 0.56 0.21 0.51 0.48 0.45
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 – – 0.05 0 0.05 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Mitochondria 0.55 0.14 0.12 0.29 0.37 0.76 0.84 0.83 0.86 0.91 0.91 0.94 0 0 0 0.08 – – 0.51 0.27 0.55 0.27 0.32 0.32
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
SpindlePole 0 0 0.1 0 0.06 0.05 0.06 0.09 0.07 0.05 0.05 0.06 0 0 0 0.16 – – 0.06 0 0.06 0 0.06 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 – – 0.08 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 – – 2 4 14 6 5 10
Bud 0 1 9 2 18 23 33 29 20 30 34 18 1 1 0 6 – – 0 0 0 3 4 7
Bud Neck 1 5 7 1 3 0 4 0 1 2 1 1 0 4 8 5 – – 1 2 5 2 3 6
Bud Site 0 0 0 0 0 9 11 13 7 12 14 12 0 0 0 0 – – – – – – – –
Cell Periphery 0 2 5 4 0 1 3 0 0 2 0 2 1 0 4 2 – – 0 1 3 2 0 3
Cytoplasm 59 246 269 184 230 190 142 94 83 51 42 37 217 326 407 138 – – 30 175 90 132 164 142
Endoplasmic Reticulum 3 4 0 0 0 2 2 1 5 3 0 0 2 1 1 18 – – 1 4 6 1 1 2
Endosome 0 1 13 3 16 0 0 1 0 0 1 0 2 1 1 12 – – 6 9 21 7 7 8
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 1 1 1 0 1
Mitochondria 63 40 41 71 140 394 563 431 409 419 376 405 0 0 0 18 – – 67 82 239 69 110 101
Nucleus 0 0 0 0 0 5 3 4 6 5 5 5 0 1 2 2 – – 0 0 0 1 2 0
Nuclear Periphery 1 0 0 0 0 1 1 0 4 5 0 2 0 0 0 0 – – 0 0 0 0 0 0
Nucleolus 0 0 0 1 0 1 1 1 1 2 0 0 1 0 0 1 – – 0 0 0 1 0 0
Peroxisomes 0 0 14 5 3 3 0 2 4 2 2 3 0 0 0 2 – – 0 0 4 0 0 0
SpindlePole 1 0 36 9 23 25 38 47 32 21 22 26 7 4 15 35 – – 7 2 26 9 19 10
Vac/Vac Membrane 5 2 4 0 9 10 17 7 5 3 16 11 1 2 2 14 – – 9 17 5 10 13 10
Unique Cell Count 114 280 353 245 380 521 669 519 478 461 414 433 229 335 426 213 132 311 434 258 345 315
Labelled Cell Count 133 301 400 280 442 664 818 630 577 557 513 522 232 340 440 254 132 311 434 258 345 315


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.1 3.4 3.5 3.3 3.4 2.9 2.9 2.9 2.9 2.7 2.7 2.7 5.0 4.9 4.8 4.9 4.8 4.8 5.0 5.3 5.5
Std Deviation (1e-4) 0.5 0.5 0.7 1.0 1.2 1.2 1.0 1.3 1.0 0.6 1.3 0.7 1.1 1.0 1.2 1.2 1.1 1.3 1.4 1.4 1.9
Intensity Change (Log2) – – – -0.08 -0.02 -0.25 -0.27 -0.26 -0.26 -0.38 -0.37 -0.35 0.51 0.48 0.46 0.5 0.47 0.46 0.5 0.59 0.66


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 – –
Bud Neck – – – – – – – – – – – – 0 – –
Bud Site – – – – – – – – – – – – 0 – –
Cell Periphery – – – – – – – – – – – – 0 – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 – –
Endosome – – – – – – – – – – – – 0 – –
Golgi – – – – – – – – – – – – 0 – –
Mitochondria – – – – – – – – – – – – 0 – –
Nuclear Periphery – – – – – – – – – – – – 0 – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 – –
Peroxisomes – – – – – – – – – – – – 0 – –
SpindlePole – – – – – – – – – – – – 0 – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 – –
Cytoplasm – – – – – – – – – – – – 0 – –
Nucleus – – – – – – – – – – – – 0 – –
Vacuole – – – – – – – – – – – – 3.6 – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.7369 0.9601 1.4228 0.6086 1.3378 1.0402 1.4036 0.2191 0.2607 0.3714 0.9244 0.1634 -0.9793 -0.8679 -1.2092 -0.9999 -0.5828 -0.7825
Actin 0.0054 0.0028 0.0047 0.0014 0.0104 0.0018 0.038 0.0009 0.0126 0.0014 0.0269 0.0038 0.023 0.0044 0.0025 0.0005 0.0166 0.008
Bud 0.0058 0.0052 0.0086 0.0002 0.0035 0.0006 0.0013 0.006 0.0007 0.0011 0.0008 0.0003 0.0009 0.0074 0.0038 0.0051 0.0015 0.0015
Bud Neck 0.007 0.0017 0.0013 0.0001 0.0376 0.0032 0.011 0.0007 0.0014 0.0008 0.0021 0.007 0.0037 0.0006 0.004 0.006 0.004 0.0138
Bud Periphery 0.0057 0.0045 0.0106 0.0011 0.0082 0.0011 0.0018 0.0048 0.0018 0.0027 0.0015 0.0007 0.0019 0.008 0.0081 0.0139 0.0021 0.0037
Bud Site 0.0372 0.0473 0.0218 0.0001 0.0101 0.0003 0.0208 0.0369 0.0094 0.0023 0.0027 0.0002 0.0034 0.0241 0.0088 0.0049 0.0027 0.0011
Cell Periphery 0.002 0.0013 0.0011 0.0003 0.0007 0.0004 0.001 0.0009 0.0009 0.0007 0.0003 0.0002 0.0005 0.0006 0.0008 0.0004 0.0002 0.0004
Cytoplasm 0.0707 0.0653 0.0805 0.0444 0.0358 0.1044 0.0853 0.1242 0.1015 0.1021 0.0768 0.204 0.0919 0.1498 0.116 0.1089 0.1442 0.1629
Cytoplasmic Foci 0.0589 0.0535 0.0297 0.01 0.0413 0.0327 0.0722 0.0476 0.03 0.0157 0.0301 0.0417 0.0787 0.0873 0.0813 0.0513 0.0469 0.0611
Eisosomes 0.001 0.001 0.0007 0.0007 0.0004 0.0002 0.0014 0.0002 0.0004 0.0002 0.0005 0.0001 0.001 0.0002 0.0005 0.0002 0.0004 0.0002
Endoplasmic Reticulum 0.0131 0.0011 0.0014 0.0019 0.0009 0.0024 0.0053 0.0025 0.0018 0.0027 0.0136 0.0026 0.0029 0.0039 0.002 0.0006 0.0038 0.0017
Endosome 0.033 0.0102 0.0147 0.0066 0.0325 0.0147 0.0416 0.0242 0.0205 0.0266 0.0289 0.0205 0.0451 0.0326 0.0335 0.0116 0.0263 0.0165
Golgi 0.0051 0.0024 0.0044 0.0029 0.0099 0.0027 0.0084 0.0028 0.0034 0.0024 0.0043 0.0039 0.0064 0.008 0.0039 0.0019 0.0051 0.0029
Lipid Particles 0.0132 0.0032 0.0058 0.0023 0.0143 0.0073 0.0276 0.0075 0.0066 0.0022 0.004 0.006 0.0141 0.0028 0.0028 0.0005 0.0123 0.0021
Mitochondria 0.0612 0.0236 0.0077 0.0284 0.0298 0.0174 0.0082 0.009 0.0201 0.0306 0.0026 0.0054 0.0303 0.0254 0.0179 0.0092 0.0168 0.0224
None 0.6419 0.754 0.7713 0.8949 0.7398 0.7918 0.5759 0.716 0.7667 0.7662 0.7274 0.6839 0.6619 0.6238 0.6942 0.7691 0.6745 0.6768
Nuclear Periphery 0.0022 0.0004 0.0022 0.0005 0.0015 0.001 0.0336 0.0015 0.0014 0.0023 0.0409 0.0035 0.0075 0.0017 0.0008 0.0003 0.0067 0.0051
Nucleolus 0.0057 0.0017 0.002 0.0002 0.0015 0.0004 0.0025 0.0007 0.0012 0.0035 0.0015 0.0005 0.0009 0.0007 0.0006 0.0012 0.0011 0.0006
Nucleus 0.0044 0.0024 0.0057 0.0004 0.0022 0.0022 0.0201 0.0018 0.0018 0.0064 0.0163 0.0048 0.0101 0.003 0.002 0.0041 0.0042 0.0026
Peroxisomes 0.0087 0.0132 0.005 0.001 0.0101 0.0092 0.0098 0.0018 0.0056 0.001 0.0012 0.0039 0.0082 0.0064 0.0093 0.0056 0.0098 0.0088
Punctate Nuclear 0.0043 0.0023 0.0184 0.0003 0.0025 0.0025 0.0279 0.0009 0.0027 0.0019 0.0129 0.0041 0.0026 0.002 0.003 0.002 0.0153 0.0044
Vacuole 0.0112 0.0026 0.002 0.0015 0.0055 0.0024 0.0044 0.008 0.0073 0.0241 0.003 0.0025 0.0038 0.0059 0.0032 0.0025 0.0041 0.0025
Vacuole Periphery 0.0021 0.0004 0.0005 0.0005 0.0013 0.0012 0.002 0.001 0.0022 0.0029 0.0019 0.0006 0.0011 0.0014 0.0011 0.0004 0.0015 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 4.5746 4.0895 2.4276 5.0552 8.3037 7.3154 4.7551 5.0233 6.264 5.3506
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1393 399 229 1572 1304 1184 135 1464 2697 1583 364 3036

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 643.02 689.71 843.18 773.43 651.31 711.09 887.33 810.02 647.03 705.70 859.55 791.07
Standard Deviation 72.46 89.33 86.78 112.89 75.73 87.90 97.71 104.78 74.18 88.75 93.45 110.58
Intensity Change Log 2 — 0.101126 0.390977 0.266407 — 0.126688 0.446126 0.314613 — 0.114045 0.418992 0.290866

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000266 0.000630 0.000694 0.004956 0.000341 0.000352 0.001485 0.005627 0.000302 0.000422 0.000987 0.005280
Bud Neck 0.027082 0.055128 0.004525 0.029935 0.023735 0.049252 0.004295 0.022517 0.025464 0.050733 0.004440 0.026358
Bud Site 0.007317 0.029319 0.004974 0.063206 0.006666 0.011604 0.014512 0.052020 0.007002 0.016069 0.008511 0.057812
Cell Periphery 0.000163 0.000166 0.000236 0.000329 0.000191 0.000235 0.000374 0.000215 0.000176 0.000218 0.000287 0.000274
Cytoplasm 0.314035* 0.230652* 0.200830* 0.307173* 0.342251* 0.268024* 0.278711* 0.335075* 0.327677* 0.258604* 0.229714* 0.320628*
Cytoplasmic Foci 0.344908* 0.337844* 0.049290 0.092264 0.339971* 0.320173* 0.110585 0.122327 0.342521* 0.324627* 0.072023 0.106761
Eisosomes 0.000245 0.000177 0.000125 0.000147 0.000229 0.000251 0.000228 0.000147 0.000237 0.000232 0.000163 0.000147
Endoplasmic Reticulum 0.001426 0.000954 0.007429 0.006049 0.001474 0.000729 0.006717 0.003298 0.001449 0.000786 0.007165 0.004722
Endosome 0.024535 0.056179 0.009828 0.016191 0.023547 0.050456 0.015227 0.012789 0.024057 0.051899 0.011831 0.014551
Golgi 0.008377 0.011463 0.000475 0.005389 0.009774 0.009924 0.000827 0.003990 0.009052 0.010312 0.000605 0.004714
Lipid Particles 0.028557 0.015146 0.010245 0.005427 0.023630 0.023021 0.017003 0.004142 0.026174 0.021036 0.012752 0.004808
Mitochondria 0.029924 0.037707 0.000940 0.011408 0.020853 0.029593 0.002439 0.005636 0.025538 0.031638 0.001496 0.008624
Mitotic Spindle 0.003146 0.007563 0.001347 0.054729 0.002426 0.005977 0.009232 0.030933 0.002798 0.006377 0.004271 0.043254
None 0.002053 0.002481 0.003720 0.001942 0.003008 0.003396 0.001742 0.002112 0.002514 0.003166 0.002986 0.002024
Nuclear Periphery 0.000862 0.000615 0.003168 0.002193 0.000612 0.000703 0.004434 0.001501 0.000741 0.000681 0.003638 0.001859
Nuclear Periphery Foci 0.000459 0.000663 0.002538 0.001815 0.000589 0.000846 0.007180 0.001614 0.000522 0.000800 0.004260 0.001718
Nucleolus 0.002641 0.004583 0.002889 0.002216 0.001948 0.004056 0.003901 0.001270 0.002306 0.004189 0.003264 0.001760
Nucleus 0.132491* 0.081335 0.595731* 0.181061* 0.138097* 0.112482 0.367910* 0.191451* 0.135201* 0.104632 0.511237* 0.186071*
Peroxisomes 0.022205 0.052820 0.008709 0.034487 0.021061 0.033007 0.012867 0.050336 0.021652 0.038001 0.010251 0.042130
Vacuole 0.047334 0.069722 0.091673 0.175885 0.037418 0.072413 0.138896 0.150562 0.042539 0.071735 0.109187 0.163674
Vacuole Periphery 0.001976 0.004852 0.000635 0.003199 0.002182 0.003502 0.001434 0.002438 0.002075 0.003842 0.000931 0.002832

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.03 -4.54 -7.67 -6.83 -6.46 -0.15 -2.62 -7.95 -7.95 -4.18 -2.30 -4.06 -11.05 -10.69 -7.86
Bud Neck -4.34 11.67 0.20 4.45 -12.44 -6.72 8.67 0.86 7.62 -9.23 -7.85 14.63 0.71 8.42 -15.82
Bud Site -5.14 1.29 -12.43 -3.56 -12.70 -3.03 -1.47 -11.98 -9.52 -5.02 -5.46 -0.56 -17.22 -11.19 -12.65
Cell Periphery -0.04 -2.97 -3.94 -3.43 -0.28 -2.01 -6.05 0.44 2.50 6.45 -2.56 -5.66 -3.15 -0.19 3.31
Cytoplasm 5.87 8.19 3.49 -3.56 -6.07 7.28 4.53 4.16 -3.35 -2.50 8.67 9.46 5.41 -4.09 -6.50
Cytoplasmic Foci 0.45 33.69 39.25 21.30 -3.98 1.99 19.18 30.78 27.59 -0.16 2.31 37.73 49.35 36.75 -3.42
Eisosomes 4.69 7.23 10.12 3.18 0.03 -1.18 -0.90 6.88 7.06 5.24 0.48 4.59 11.96 7.89 3.38
Endoplasmic Reticulum 1.11 -7.29 -10.72 -13.74 2.21 3.04 -6.33 -7.85 -15.01 3.80 3.11 -9.56 -13.24 -20.68 4.23
Endosome -6.36 6.24 7.16 9.16 -1.35 -8.27 2.71 6.60 14.32 2.47 -10.62 6.56 9.70 16.86 0.36
Golgi -1.63 8.78 4.85 4.84 -5.05 -0.04 8.44 6.19 7.63 -3.43 -1.17 12.21 7.83 8.94 -6.17
Lipid Particles 5.20 6.89 13.53 5.28 1.91 0.13 2.63 10.92 12.20 4.85 2.75 7.01 17.30 13.05 4.29
Mitochondria -1.21 10.98 7.85 5.59 -7.29 -2.75 8.68 7.90 10.54 -2.36 -2.09 14.19 10.95 11.28 -7.58
Mitotic Spindle -2.38 1.29 -11.79 -9.67 -12.20 -2.45 -1.53 -8.56 -6.99 -3.45 -3.15 -0.88 -14.51 -12.35 -11.69
None -0.43 -1.38 0.76 0.82 1.87 -0.44 3.98 3.11 1.76 -1.33 -0.87 0.06 2.70 1.91 1.54
Nuclear Periphery 1.69 -7.49 -8.43 -9.08 1.59 -1.08 -2.62 -6.09 -5.44 1.69 0.73 -5.48 -10.37 -10.63 2.43
Nuclear Periphery Foci -1.61 -4.48 -6.24 -4.62 1.16 -0.77 -4.17 -5.46 -2.44 3.33 -1.16 -5.72 -8.28 -3.65 3.46
Nucleolus -1.82 0.13 1.13 2.36 0.70 -3.16 -1.54 2.18 3.96 2.33 -3.27 -0.99 2.06 4.18 2.19
Nucleus 5.64 -21.42 -9.54 -12.20 17.89 3.82 -9.43 -8.66 -12.18 6.54 5.88 -21.74 -12.86 -16.89 17.52
Peroxisomes -6.35 4.82 -1.07 5.57 -5.15 -4.57 2.35 -5.14 -1.44 -5.75 -7.37 5.23 -4.68 2.59 -7.98
Vacuole -3.71 -6.36 -30.44 -20.85 -9.46 -9.44 -9.26 -29.26 -22.02 -2.32 -9.41 -10.74 -42.17 -31.57 -8.66
Vacuole Periphery -4.00 9.14 -0.08 3.34 -3.11 -4.47 2.14 1.44 4.24 -0.70 -6.47 6.26 0.87 5.18 -2.90
Standard name
Human Ortholog
Description Methionyl-tRNA formyltransferase; catalyzes the formylation of initiator Met-tRNA in mitochondria; potential Cdc28p substrate
Localization
Cell Percentages mitochondrion (18%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Fmt1

Fmt1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fmt1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available