Standard name
Human Ortholog
Description Protein with CoA transferase activity; particularly for CoASH transfer from succinyl-CoA to acetate; has minor acetyl-CoA-hydrolase activity; phosphorylated; required for acetate utilization and for diploid pseudohyphal growth
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0.06
Bud 0 0.05 0.05 0.08 0.06 0 0.05 0.08 0.11 0.09 0 0 0 0 0 0.05 – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – –
Cell Periphery 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 – – 0 0 0 0 0 0
Cytoplasm 0 0.08 0.29 0.4 0.18 0.1 0.08 0.09 0 0 0.06 0 0.84 0.71 0.6 0.05 – – 0 0 0 0.11 0.11 0.12
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Endosome 0.31 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.1 0.12 – – 0.1 0.1 0.07 0.07 0.08 0.09
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Mitochondria 0.48 0.88 0.72 0.62 0.73 0.9 0.9 0.84 0.92 0.93 0.92 0.91 0.12 0.16 0.24 0.07 – – 0.75 0.71 0.72 0.53 0.55 0.44
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0.09 0.05 0.07 0.07 0 0.14 0 0 0 0.07 – – 0 0 0 0 0 0
Nucleolus 0.05 0 0 0 0 0 0 0.07 0.06 0.1 0 0.07 0 0 0 0.15 – – 0 0 0 0 0 0
Peroxisomes 0.22 0 0 0.07 0 0 0 0 0 0 0 0 0 0.06 0.07 0.4 – – 0 0 0.06 0 0 0
SpindlePole 0.07 0 0 0.09 0.09 0 0.05 0.05 0 0 0.06 0 0 0.05 0 0.2 – – 0 0 0 0.08 0.07 0.06
Vac/Vac Membrane 0 0.13 0 0 0.05 0 0 0 0 0 0.06 0 0 0 0 0.1 – – 0 0.07 0 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 3 1 0 0 0 0 0 1 0 0 0 0 0 1 – – 1 4 5 16 23 42
Bud 1 17 9 16 17 14 21 27 32 28 2 2 9 18 14 2 – – 0 1 2 15 13 25
Bud Neck 1 0 3 0 3 2 1 1 2 2 0 0 3 0 0 0 – – 1 2 1 6 7 23
Bud Site 1 1 1 0 1 0 14 6 4 4 1 1 0 0 0 1 – – – – – – – –
Cell Periphery 9 5 1 3 2 1 2 2 5 5 0 0 2 1 2 3 – – 0 0 0 2 2 9
Cytoplasm 5 27 56 80 54 32 31 29 11 6 3 2 388 548 407 2 – – 1 5 5 66 73 83
Endoplasmic Reticulum 0 7 7 0 1 2 0 0 1 1 0 1 0 1 7 0 – – 4 1 1 7 7 6
Endosome 58 9 0 3 11 2 0 3 1 0 0 0 14 68 71 5 – – 33 35 14 38 50 64
Golgi 0 0 0 0 1 0 0 1 0 0 0 0 0 2 1 0 – – 6 6 1 9 13 3
Mitochondria 89 299 141 124 213 296 363 286 267 292 45 74 57 121 161 3 – – 251 255 160 302 359 309
Nucleus 5 1 0 0 3 5 7 4 3 7 0 1 0 1 2 1 – – 0 0 0 1 0 0
Nuclear Periphery 4 3 0 2 6 11 35 18 21 23 2 11 0 0 2 3 – – 1 0 0 2 4 5
Nucleolus 9 2 0 0 2 12 17 23 18 30 2 6 1 4 1 6 – – 1 1 0 2 6 5
Peroxisomes 41 5 4 13 11 5 3 8 3 6 1 0 5 43 49 16 – – 13 6 13 19 15 13
SpindlePole 14 5 4 17 25 10 19 17 4 10 3 3 14 35 20 8 – – 2 3 5 44 45 44
Vac/Vac Membrane 5 45 3 2 14 10 10 10 12 11 3 1 14 23 24 4 – – 6 26 3 19 14 42
Unique Cell Count 187 340 196 200 293 328 404 339 289 315 49 81 463 777 679 40 333 358 222 575 659 703
Labelled Cell Count 242 426 232 261 364 402 523 435 384 426 62 102 507 865 761 55 333 358 222 575 659 703


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 4.3 3.5 3.5 4.0 3.8 3.9 5.3 6.6 7.3 8.9 8.1 4.0 4.0 4.2 9.9 – – 4.1 4.2 4.7
Std Deviation (1e-4) 1.2 0.8 0.8 2.0 1.5 1.5 1.9 3.4 5.3 5.8 7.2 5.4 1.0 1.0 1.2 3.6 – – 1.5 1.6 1.8
Intensity Change (Log2) – – – -0.02 0.2 0.11 0.14 0.6 0.92 1.06 1.34 1.21 0.19 0.19 0.27 1.5 – – 0.21 0.26 0.43


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 12.3458 12.1385 12.0161 10.9213 14.0489 12.2138 7.6188 8.202 8.1393 9.2764 6.8666 6.2583 7.2306 10.9245 8.7479 8.8982 8.7716 9.165
Actin 0.0235 0.0028 0.0175 0.0012 0.0017 0.0032 0.0294 0.0865 0.022 0.0002 0.0004 0.0325 0.0968 0.0036 0.0588 0.0002 0.0011 0.0102
Bud 0.0004 0.0001 0.0007 0.0009 0 0.0001 0.0003 0.0002 0.0004 0.0012 0 0.0001 0.001 0.0001 0.0006 0.0002 0.0002 0.0004
Bud Neck 0.0045 0 0.0002 0 0 0 0.0003 0.0001 0.0002 0.0001 0 0.0001 0.0005 0.0002 0.0005 0.0002 0.0002 0.0003
Bud Periphery 0.001 0.0002 0.0012 0.0051 0.0002 0.0002 0.0006 0.0003 0.0016 0.0015 0.0002 0.0002 0.0022 0.0003 0.0014 0.0003 0.0024 0.0006
Bud Site 0.0029 0.0002 0.0042 0.0002 0.0001 0 0.0007 0.0007 0.0061 0.0011 0.0001 0.0002 0.0024 0.0006 0.002 0.0001 0.0001 0.0004
Cell Periphery 0.0015 0.0001 0.0005 0.0006 0.0001 0.0001 0.0001 0.0001 0.0089 0.0002 0.0002 0.0001 0.0002 0.0001 0.0009 0.0001 0.0017 0.0001
Cytoplasm 0.0117 0 0.0003 0.0001 0 0 0.0001 0 0.0001 0.0004 0 0 0.0002 0 0.0001 0.0001 0.0006 0
Cytoplasmic Foci 0.0106 0.0018 0.0037 0.0002 0.0005 0.0003 0.0167 0.0039 0.0012 0.0117 0.0037 0.0013 0.0286 0.0077 0.0009 0.0016 0.0005 0.0015
Eisosomes 0.0004 0.0002 0.0005 0.0001 0.0002 0.0002 0.0018 0.0004 0.0041 0 0.0001 0.0003 0.0011 0.0002 0.0012 0 0.0003 0.0002
Endoplasmic Reticulum 0.0016 0 0.0001 0.0001 0 0.0001 0.0005 0 0.0001 0.0002 0 0 0.0009 0 0.0004 0.0002 0.0063 0
Endosome 0.0165 0.0061 0.0064 0.0011 0.001 0.0026 0.0092 0.0086 0.0027 0.0431 0.0022 0.0021 0.0176 0.0035 0.0018 0.0748 0.0071 0.0025
Golgi 0.0192 0.0232 0.0047 0.0009 0.0028 0.0269 0.0182 0.0144 0.0162 0.0213 0.0019 0.0152 0.0521 0.0041 0.0043 0.0248 0.0045 0.0052
Lipid Particles 0.0244 0.0028 0.0053 0.0023 0.0297 0.0071 0.0435 0.0017 0.0041 0.0038 0.0595 0.0045 0.1945 0.0081 0.0016 0.0014 0.0009 0.0142
Mitochondria 0.8023 0.9421 0.8924 0.9785 0.948 0.9524 0.7534 0.8474 0.9189 0.8961 0.9162 0.9173 0.4771 0.9122 0.9133 0.8799 0.9585 0.9348
None 0.0117 0 0.0002 0 0.0001 0 0.0002 0 0.0001 0.0001 0 0 0.0015 0 0.0001 0 0.0003 0
Nuclear Periphery 0.0063 0 0.0034 0.0001 0.0007 0.0002 0.034 0 0.0001 0.0002 0 0 0.0034 0.0001 0.0002 0.0002 0.0009 0.0001
Nucleolus 0.0209 0.0001 0.0005 0 0.0008 0 0.0002 0 0.0002 0.0004 0.0004 0 0.0003 0.0077 0.0001 0.0001 0 0.0003
Nucleus 0.0027 0 0.0011 0.0001 0.0005 0 0.0009 0 0.0001 0.0003 0.0001 0 0.0002 0.0002 0.0001 0.0001 0.0002 0.0001
Peroxisomes 0.0273 0.0121 0.0236 0.0043 0.006 0.0034 0.0817 0.0349 0.0034 0.0035 0.0129 0.0248 0.1146 0.0478 0.003 0.0003 0.0064 0.0221
Punctate Nuclear 0.0018 0 0.0047 0 0 0 0.0003 0 0 0.0001 0 0 0.0025 0 0 0 0 0
Vacuole 0.0032 0.0009 0.0106 0.0017 0.0011 0.0005 0.0008 0.0003 0.0015 0.0075 0.0009 0.0002 0.0006 0.0014 0.004 0.0088 0.0041 0.0018
Vacuole Periphery 0.0058 0.0073 0.0182 0.0026 0.0063 0.0026 0.0073 0.0004 0.0081 0.0069 0.0011 0.001 0.0016 0.0021 0.0046 0.0066 0.0039 0.0051

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 23.0312 19.3407 12.4998 14.1078 18.55 25.111 14.0357 11.9213 17.4461 15.6211
Translational Efficiency 1.0504 0.7351 1.0023 1.241 1.1854 1.2992 0.9941 1.5887 1.3232 1.3012

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein with CoA transferase activity; particularly for CoASH transfer from succinyl-CoA to acetate; has minor acetyl-CoA-hydrolase activity; phosphorylated; required for acetate utilization and for diploid pseudohyphal growth
Localization
Cell Percentages mitochondrion (22%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Ach1

Ach1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ach1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available