ORF
Human Ortholog
Description Non-essential protein of unknown function
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0.93 0.97 0.98 0.99 0.95 0.87 0.74 0.8 0.6 0.63 0.66 0.62 0.99 1.0 1.0 – – – 0.86 0.88 0.83 0.9 0.88 0.88
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0.11 0 0 0 0 0.17 0.33 0.23 0.51 0.35 0.51 0.32 0 0 0 – – – 0 0 0.06 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.06 0.06 0.1 0.06 0.08 0.12 0.1 0.16 0 0 0 – – – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 3 2 3 0 2 1
Bud 1 1 0 0 1 2 6 4 5 9 16 7 0 0 0 – – – 5 1 0 0 5 5
Bud Neck 1 0 5 3 0 0 2 3 2 2 0 1 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 3 0 2 0 0 0 – – – – – – – – –
Cell Periphery 0 0 1 2 0 3 1 2 1 4 0 0 1 5 2 – – – 0 0 2 0 0 0
Cytoplasm 387 221 283 243 402 342 395 378 202 320 261 211 161 208 225 – – – 368 200 228 85 169 161
Endoplasmic Reticulum 0 2 0 0 0 1 0 0 1 1 0 0 0 0 1 – – – 0 1 4 0 2 2
Endosome 1 1 0 0 1 1 1 0 0 2 0 1 0 0 0 – – – 7 1 4 0 1 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 2 0 0 0 1 0
Mitochondria 45 0 1 2 10 67 174 108 170 175 203 108 0 0 0 – – – 12 4 17 4 4 3
Nucleus 1 1 1 2 4 7 13 13 7 27 11 19 2 0 0 – – – 3 1 0 0 0 0
Nuclear Periphery 0 3 0 0 0 1 0 0 1 0 1 0 0 0 0 – – – 0 0 0 0 1 0
Nucleolus 0 1 1 0 0 0 1 0 0 1 0 0 1 0 0 – – – 0 0 0 0 0 0
Peroxisomes 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 2 0 0 0 0 0
SpindlePole 0 0 1 0 0 1 1 2 0 2 1 1 0 0 0 – – – 6 2 2 0 0 2
Vac/Vac Membrane 3 1 1 1 25 23 52 27 27 61 38 53 0 0 0 – – – 6 9 1 2 0 1
Unique Cell Count 415 227 288 246 421 391 532 470 336 504 395 338 162 208 226 430 228 277 95 191 184
Labelled Cell Count 440 231 294 253 443 448 646 537 416 607 531 403 165 213 228 430 228 277 95 191 184


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.4 4.6 4.9 4.7 5.0 4.1 4.1 4.6 3.9 4.2 3.8 4.3 4.9 5.2 5.2 – – – 4.8 5.4 5.9
Std Deviation (1e-4) 0.5 1.1 1.3 1.6 1.6 1.3 1.2 1.4 1.1 1.2 1.3 1.1 1.0 1.2 1.1 – – – 1.3 1.7 2.3
Intensity Change (Log2) – – – -0.08 0.02 -0.27 -0.29 -0.11 -0.34 -0.24 -0.4 -0.22 -0.03 0.07 0.08 – – – -0.06 0.13 0.26


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.9647 1.2394 0.9631 0.9627 0.734 1.0314 -0.5446 -0.044 -0.1529 -0.383 -0.2327 -0.1836 0.0301 0.249 0.0666 0.4926 -0.0881 -0.1106
Actin 0.0392 0.0021 0.0101 0.0043 0.0316 0.0011 0.0307 0.0003 0.0044 0.0002 0.012 0.0043 0.0155 0.0003 0.0004 0.0115 0.0004 0.0029
Bud 0.0008 0.0023 0.0003 0.0008 0.0036 0.0002 0.0051 0.0021 0.0004 0.0001 0.0015 0.0006 0.0005 0.0047 0.0072 0.0006 0.0003 0.0009
Bud Neck 0.0043 0.0004 0.0015 0.0007 0.0008 0.0006 0.0032 0.0003 0.0116 0.0001 0.0042 0.0012 0.0017 0.0016 0.0002 0.0007 0.0003 0.0018
Bud Periphery 0.002 0.0009 0.0005 0.001 0.0083 0.0009 0.0018 0.0019 0.0007 0.0002 0.0021 0.0023 0.0004 0.0019 0.0018 0.0007 0.0003 0.0005
Bud Site 0.0035 0.0251 0.0012 0.0005 0.0038 0.0001 0.0048 0.0083 0.0039 0.0001 0.0073 0.0002 0.0032 0.0228 0.0015 0.0003 0.0001 0.0002
Cell Periphery 0.0003 0.0004 0.0001 0.0001 0.0003 0.0001 0.0003 0.0002 0.0002 0 0.0002 0.0001 0.0002 0.0003 0.0001 0 0 0.0001
Cytoplasm 0.1741 0.3777 0.3231 0.3523 0.2267 0.4243 0.1832 0.2903 0.2449 0.1792 0.1981 0.3055 0.2961 0.4481 0.4052 0.3497 0.474 0.4107
Cytoplasmic Foci 0.0324 0.0156 0.0135 0.0259 0.0087 0.0087 0.0145 0.004 0.013 0.0114 0.0126 0.0108 0.0115 0.0092 0.007 0.0179 0.0126 0.0178
Eisosomes 0.0004 0.0001 0.0002 0.0001 0.0005 0.0001 0.0006 0.0001 0.0001 0.0001 0.0003 0.0001 0.0004 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0032 0.0024 0.0033 0.0037 0.0049 0.0017 0.0029 0.0018 0.0015 0.0013 0.0018 0.0014 0.0036 0.002 0.0023 0.002 0.0017 0.0025
Endosome 0.0251 0.003 0.0046 0.0057 0.037 0.004 0.0068 0.001 0.0029 0.0046 0.0038 0.002 0.0101 0.0017 0.0017 0.0048 0.0048 0.0059
Golgi 0.0063 0.0006 0.0007 0.0006 0.0097 0.0017 0.0024 0.0001 0.0011 0.0001 0.0014 0.0003 0.0027 0.0001 0.0001 0.0044 0.0001 0.0003
Lipid Particles 0.012 0.0004 0.0012 0.001 0.0112 0.001 0.0037 0.0002 0.0017 0.0002 0.0026 0.0006 0.0037 0.0002 0.0002 0.0045 0.0002 0.0006
Mitochondria 0.0071 0.0006 0.0009 0.0012 0.0209 0.0036 0.0018 0.0004 0.0013 0.0005 0.0022 0.0007 0.0027 0.0003 0.0016 0.0008 0.0004 0.0008
None 0.6436 0.537 0.601 0.5229 0.5608 0.5377 0.7112 0.6803 0.6734 0.7925 0.6744 0.6388 0.6151 0.4815 0.5392 0.5545 0.4582 0.5087
Nuclear Periphery 0.0109 0.0024 0.003 0.0081 0.0288 0.0009 0.0045 0.001 0.0034 0.0012 0.0056 0.0014 0.0109 0.0014 0.0027 0.0038 0.0029 0.0038
Nucleolus 0.0015 0.0005 0.0008 0.0007 0.002 0.0001 0.0019 0.0003 0.0006 0.0002 0.0076 0.0003 0.001 0.0004 0.0007 0.0005 0.0003 0.0007
Nucleus 0.0054 0.0169 0.0173 0.0452 0.011 0.0081 0.0072 0.0052 0.0207 0.0041 0.02 0.0177 0.0107 0.0181 0.0221 0.0134 0.0368 0.0198
Peroxisomes 0.0102 0.0013 0.0064 0.0026 0.0087 0.0009 0.0024 0.0001 0.002 0.0004 0.0037 0.0034 0.0019 0.0001 0.0003 0.0245 0.0002 0.0024
Punctate Nuclear 0.0139 0.0089 0.0086 0.021 0.0136 0.0035 0.0095 0.0011 0.011 0.0021 0.0375 0.0075 0.0057 0.0038 0.0044 0.0037 0.0046 0.0177
Vacuole 0.0023 0.001 0.0015 0.0012 0.0036 0.0005 0.001 0.0009 0.0009 0.001 0.0009 0.0006 0.0015 0.0012 0.001 0.0015 0.0012 0.0014
Vacuole Periphery 0.0012 0.0002 0.0002 0.0004 0.0035 0.0002 0.0004 0.0001 0.0002 0.0002 0.0003 0.0001 0.0008 0.0001 0.0002 0.0003 0.0002 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression – – – – – – – – – –
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression –
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
40 594 3037 1246 1439 2317 1886 1013 1479 2911 4923 2259

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 964.66 639.38 766.38 794.49 639.67 681.05 770.10 854.01 648.46 672.55 767.81 821.18
Standard Deviation 392.78 102.45 88.08 122.77 75.34 84.07 100.41 156.13 111.69 89.72 93.02 141.85
Intensity Change Log 2 — -0.593347 -0.331961 -0.279991 — 0.090433 0.267718 0.416925 — -0.280963 -0.062342 0.039183

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001093 0.000494 0.000111 0.000734 0.000082 0.000245 0.000193 0.001718 0.000109 0.000296 0.000142 0.001175
Bud Neck 0.062172 0.026550 0.013262 0.011646 0.012520 0.033095 0.005782 0.011970 0.013863 0.031760 0.010396 0.011791
Bud Site 0.085558 0.013061 0.012606 0.024962 0.001720 0.017411 0.013899 0.064508 0.003988 0.016523 0.013101 0.042695
Cell Periphery 0.001098 0.000151 0.000127 0.000235 0.000118 0.000138 0.000265 0.000229 0.000144 0.000141 0.000180 0.000232
Cytoplasm 0.288620* 0.376788* 0.237739* 0.360548* 0.479289* 0.466457* 0.222911* 0.459379* 0.474132* 0.448160* 0.232059* 0.404866*
Cytoplasmic Foci 0.059204 0.270363* 0.001593 0.007839 0.189847* 0.148417 0.007710 0.016755 0.186314* 0.173300 0.003936 0.011837
Eisosomes 0.000140 0.000153 0.000014 0.000044 0.000134 0.000085 0.000033 0.000040 0.000134 0.000099 0.000021 0.000042
Endoplasmic Reticulum 0.014436 0.001018 0.001281 0.005607 0.000550 0.000568 0.001275 0.001775 0.000925 0.000660 0.001278 0.003889
Endosome 0.018741 0.030551 0.000732 0.003139 0.007326 0.011484 0.001561 0.011071 0.007635 0.015375 0.001049 0.006696
Golgi 0.000969 0.006609 0.000124 0.000834 0.001100 0.002588 0.000122 0.010603 0.001097 0.003409 0.000123 0.005215
Lipid Particles 0.007441 0.013415 0.000303 0.001386 0.008762 0.004126 0.000772 0.001082 0.008726 0.006022 0.000483 0.001250
Mitochondria 0.003570 0.013305 0.001369 0.001697 0.003634 0.008203 0.001816 0.004408 0.003632 0.009244 0.001540 0.002912
Mitotic Spindle 0.001512 0.003165 0.005036 0.012458 0.002131 0.010725 0.006776 0.060325 0.002114 0.009183 0.005703 0.033923
None 0.004522 0.007343 0.004617 0.007767 0.004624 0.004563 0.012542 0.009507 0.004622 0.005130 0.007653 0.008547
Nuclear Periphery 0.013722 0.000667 0.001797 0.002943 0.000641 0.000543 0.001220 0.002069 0.000995 0.000568 0.001576 0.002551
Nuclear Periphery Foci 0.050954 0.001023 0.000105 0.002203 0.000443 0.000189 0.000250 0.001820 0.001809 0.000359 0.000161 0.002031
Nucleolus 0.008065 0.004964 0.000513 0.000684 0.001440 0.002060 0.001100 0.000567 0.001619 0.002652 0.000738 0.000632
Nucleus 0.237646* 0.134657* 0.706236* 0.480893* 0.252646* 0.226792* 0.704237* 0.220366* 0.252240* 0.207992* 0.705471* 0.364065*
Peroxisomes 0.002588 0.024344 0.000080 0.000353 0.004926 0.008255 0.000379 0.002347 0.004863 0.011538 0.000194 0.001247
Vacuole 0.128929 0.067454 0.011678 0.072613 0.027422 0.052714 0.016368 0.110796 0.030167 0.055721 0.013475 0.089736
Vacuole Periphery 0.009021 0.003924 0.000678 0.001415 0.000645 0.001342 0.000789 0.008665 0.000871 0.001869 0.000720 0.004666

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.66 2.68 1.19 -1.11 -4.48 -6.15 -4.07 -2.34 -1.94 -2.03 -5.50 -2.67 -3.73 -2.75 -3.46
Bud Neck 1.43 2.01 2.14 5.67 2.06 -9.39 4.64 2.91 12.84 -2.27 -8.63 2.35 3.04 13.66 1.07
Bud Site 2.70 2.76 2.61 -0.74 -1.45 -10.45 -5.66 -8.46 -3.79 -5.11 -8.51 -5.94 -8.72 -2.63 -4.96
Cell Periphery 1.88 1.95 1.79 -2.85 -4.70 -1.93 -3.45 -3.09 -1.48 1.95 0.22 -0.80 -2.13 -3.52 -1.22
Cytoplasm -2.05 1.62 0.10 6.68 -7.62 1.46 31.13 10.39 9.74 -14.99 3.13 34.37 17.67 16.76 -15.85
Cytoplasmic Foci -13.32 4.27 3.86 28.94 -9.87 6.61 35.55 34.68 35.91 -3.13 2.19 36.56 35.45 45.20 -8.53
Eisosomes -0.31 2.39 1.56 6.49 -18.48 3.32 6.68 5.64 9.56 -5.90 2.40 7.78 5.54 10.65 -18.80
Endoplasmic Reticulum 3.99 3.78 2.34 -13.97 -12.34 -0.33 -10.91 -13.69 -13.52 -5.71 2.04 -6.21 -15.56 -18.34 -13.38
Endosome -2.17 3.93 3.57 11.10 -5.20 -3.95 7.76 2.51 6.34 -3.97 -7.11 9.18 5.25 13.68 -5.95
Golgi -4.64 2.87 1.55 5.20 -1.09 -3.34 5.74 -2.15 -0.74 -3.13 -5.23 5.81 -1.21 2.57 -3.31
Lipid Particles -2.39 3.88 3.19 7.69 -13.82 6.11 10.86 10.18 12.33 -5.47 3.48 11.53 10.18 12.41 -14.87
Mitochondria -3.51 1.39 1.37 5.09 -0.07 -4.54 2.86 1.87 6.92 -0.93 -5.53 3.31 2.79 9.53 -0.92
Mitotic Spindle -1.70 -3.96 -5.00 -4.04 -2.62 -5.17 -2.59 -8.91 -6.80 -7.86 -5.05 -3.62 -9.71 -6.33 -8.10
None -1.19 -0.05 -0.77 0.85 -2.43 0.10 -4.73 -2.26 -2.61 2.49 -0.50 -2.54 -2.01 -1.74 0.60
Nuclear Periphery 1.73 1.51 1.31 -15.10 -7.51 1.35 -9.67 -7.38 -7.72 -4.15 1.92 -4.70 -8.75 -16.08 -7.63
Nuclear Periphery Foci 3.11 3.16 3.03 -2.46 -5.43 2.19 1.13 -3.54 -5.03 -4.49 2.97 3.28 -0.14 -6.20 -6.98
Nucleolus 1.25 2.93 2.97 4.68 0.47 -1.58 1.42 2.96 7.12 3.45 -2.44 2.52 3.45 8.13 1.74
Nucleus 2.46 -12.22 -6.56 -32.97 23.09 3.43 -52.52 -3.98 -6.93 39.68 6.18 -67.10 -18.21 -26.63 42.09
Peroxisomes -6.75 4.62 4.25 7.57 -4.50 -4.27 9.28 6.69 10.68 -1.20 -7.00 9.86 8.65 13.46 -1.88
Vacuole 2.32 4.20 -0.32 -10.15 -21.52 -11.17 0.05 -23.13 -19.53 -22.82 -11.22 5.52 -28.72 -22.93 -31.24
Vacuole Periphery 1.23 2.06 2.02 3.93 -0.36 -3.28 0.09 -2.72 -1.91 -2.76 -3.58 1.21 -1.74 0.41 -2.59
ORF
Human Ortholog
Description Non-essential protein of unknown function
Localization
Cell Percentages cytoplasm (10%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YBL029W

YBL029W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ybl029w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available