Standard name
Human Ortholog
Description RNA binding protein involved in asymmetric localization of ASH1 mRNA; represses translation of ASH1 mRNA, an effect reversed by Yck1p-dependent phosphoryation; regulates telomere position effect and length; similarity to hnRNP-K

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.1 0.07 0.07 0.07 0.08 0.1 0.06 0.1 0.08 0.12 0.1 0 0 0 0 0 0 0
Bud 0 0.06 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0.05 0.06 0 0.05 0.08 0 0 0 0 0 0 0 0 0
Cytoplasm 0.73 0.64 0.69 0.62 0.49 0.37 0.39 0.44 0.35 0.39 0.6 0.67 0.86 0.89 0.91 0.64 0.75 0.68 0.61
Endoplasmic Reticulum 0.17 0.16 0.14 0.2 0.23 0.2 0.32 0.28 0.15 0.24 0.14 0.1 0.06 0 0.08 0.17 0.17 0.22 0.32
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.09 0.15 0.13 0.06 0 0.18 0.08 0.06 0.08 0.05 0.25 0.2 0.07 0.06 0 0 0 0 0
Mitochondria 0 0.12 0.1 0.21 0.28 0.21 0.29 0.31 0.42 0.35 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.12 0 0 0.06 0 0 0.08 0 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 8 19 20 23 28 36 33 23 36 33 46 33 10 1 1 7 1 4 4
Bud 11 11 7 2 3 6 2 1 8 7 19 8 8 3 0 6 0 6 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 10 4 9 11 14 20 19 15 20 30 7 7 7 9 5 2 0 0 0
Cytoplasm 202 116 211 203 203 163 131 166 131 156 226 231 368 185 105 205 114 244 239
Endoplasmic Reticulum 47 30 44 67 96 88 108 105 57 95 51 34 24 8 9 55 25 78 125
Endosome 0 0 1 0 0 1 0 0 0 0 2 2 2 0 0 1 1 1 2
Golgi 24 28 39 21 18 81 26 23 28 20 92 68 29 12 4 11 0 3 3
Mitochondria 4 21 29 70 118 93 96 118 154 140 1 0 1 3 1 10 1 1 2
Nucleus 0 0 0 0 0 1 1 0 1 0 1 0 0 0 0 0 1 1 0
Nuclear Periphery 0 0 0 0 0 1 0 0 0 3 0 0 0 0 1 2 0 1 0
Nucleolus 0 0 0 0 0 3 0 0 0 1 2 0 0 0 1 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Vac/Vac Membrane 6 8 11 3 10 54 12 12 23 17 6 26 5 6 6 7 3 9 7
Unique Cell Count 277 182 305 330 416 440 336 377 371 398 374 346 426 208 116 319 154 360 394
Labelled Cell Count 312 238 371 400 490 547 428 463 458 502 453 410 454 227 133 319 154 360 394


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 19.3 17.6 17.0 16.4 16.0 16.1 15.4 14.9 14.4 14.7 21.6 21.8 20.4 38.5 36.9 18.9 20.1 20.1
Std Deviation (1e-4) 3.4 3.1 3.4 3.3 3.1 3.4 3.2 3.5 3.4 3.5 4.1 4.6 4.4 11.1 10.7 4.4 4.3 4.1
Intensity Change (Log2) -0.13 -0.18 -0.23 -0.27 -0.26 -0.32 -0.37 -0.42 -0.39 0.16 0.18 0.08 1.0 0.94 -0.03 0.06 0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 3.4 2.1 2.3 2.2 2.9 3.4 1.9 3.4 2.9 4.3 3.3 -0.4 0 0
Bud 1.0 -1.2 -2.9 -3.0 -2.2 -2.9 -3.5 -1.4 -1.8 0.7 -1.2 -1.7 -1.6 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.9 -0.4 -0.2 -0.2 0.6 1.2 0.2 1.1 2.1 -1.4 -1.2 -1.7 0.4 0
Cytoplasm -2.1 -1.0 -3.0 -6.3 -9.4 -8.4 -7.4 -9.5 -8.6 -3.3 -1.7 4.5 4.3 3.8
Endoplasmic Reticulum -0.1 -0.8 1.0 1.9 1.0 4.3 3.3 -0.6 2.2 -1.2 -2.6 -4.9 -4.5 -2.4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 2.2 1.6 -1.1 -2.3 3.6 -0.4 -1.3 -0.5 -1.9 5.3 3.8 -0.9 -1.2 -1.8
Mitochondria 4.7 4.2 7.4 9.1 7.5 9.0 9.7 11.8 10.5 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 1.4 1.0 0 0.2 4.8 1.0 0.8 2.5 1.5 -0.5 3.0 0 0.5 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 14.3928 19.0236 17.909 18.5471 19.5784 18.9879 17.2345 22.8256 20.9403 15.7506 20.9489 21.8767 17.4528 21.5923 20.847 19.3355 18.5871 20.7331
Actin 0.0312 0.0041 0.0121 0.0009 0.0007 0.0029 0.0123 0.0012 0.0124 0.0182 0.0044 0.0056 0.0145 0.0025 0.008 0.0073 0.0178 0.0031
Bud 0.0026 0.0026 0.0351 0.0094 0.0034 0.0009 0.0036 0.001 0.0084 0.0032 0.0041 0.0027 0.0038 0.0021 0.0121 0.0046 0.0012 0.0007
Bud Neck 0.003 0.0009 0.0009 0.001 0.0008 0.0031 0.0029 0.0006 0.0014 0.0073 0.0025 0.0042 0.0012 0.0005 0.0042 0.0011 0.0012 0.0017
Bud Periphery 0.0021 0.0011 0.0148 0.0121 0.0008 0.0007 0.0028 0.0002 0.0043 0.003 0.0026 0.005 0.0026 0.0004 0.0078 0.0027 0.0004 0.0005
Bud Site 0.0064 0.0095 0.0058 0.0006 0.0003 0.0002 0.0089 0.0014 0.0073 0.0122 0.0104 0.0003 0.0014 0.0135 0.0106 0.002 0.0003 0.0002
Cell Periphery 0.0022 0.0005 0.0008 0.0003 0.0001 0.0005 0.0014 0.0008 0.0008 0.0007 0.0024 0.0004 0.001 0.0002 0.0004 0.0003 0.0001 0.0004
Cytoplasm 0.4987 0.8286 0.7151 0.6467 0.6839 0.6233 0.6199 0.9166 0.8066 0.4702 0.6731 0.7571 0.6637 0.9041 0.777 0.6749 0.7482 0.7149
Cytoplasmic Foci 0.0513 0.0384 0.0296 0.0229 0.034 0.0407 0.0362 0.0122 0.019 0.0389 0.025 0.0252 0.0223 0.0152 0.0239 0.0375 0.0302 0.0305
Eisosomes 0.0004 0 0.0001 0 0 0 0.0001 0 0.0001 0.0004 0.0003 0 0.0001 0 0 0.0001 0.0001 0
Endoplasmic Reticulum 0.0843 0.0338 0.0184 0.0356 0.0071 0.0902 0.0696 0.0188 0.0123 0.0416 0.011 0.0488 0.0804 0.0137 0.0173 0.0117 0.003 0.0539
Endosome 0.145 0.0491 0.0844 0.1678 0.1628 0.1436 0.0924 0.0241 0.0537 0.1317 0.18 0.0919 0.1005 0.0294 0.0609 0.1444 0.1004 0.1338
Golgi 0.0505 0.0135 0.0209 0.0164 0.0265 0.0541 0.0278 0.0047 0.0127 0.0184 0.0178 0.0199 0.0134 0.0068 0.0112 0.0299 0.0278 0.0212
Lipid Particles 0.0092 0.0015 0.0053 0.0001 0.0001 0.0009 0.0222 0.0013 0.0029 0.0259 0.0037 0.0025 0.0067 0.0001 0.0027 0.0089 0.0227 0.0013
Mitochondria 0.028 0.0029 0.022 0.0027 0.0029 0.0169 0.0162 0.0008 0.0131 0.0469 0.0124 0.0135 0.0174 0.0011 0.0169 0.0156 0.0039 0.0103
None 0.0092 0.0006 0.0023 0.0004 0.0004 0.0006 0.0028 0.0003 0.0005 0.0036 0.0006 0.0005 0.0043 0.0003 0.0008 0.0012 0.0004 0.0018
Nuclear Periphery 0.0237 0.001 0.0027 0.0032 0.0042 0.0022 0.0165 0.0014 0.0037 0.0174 0.0031 0.0029 0.0153 0.0012 0.0047 0.0078 0.0021 0.0065
Nucleolus 0.0008 0.0015 0.0001 0 0 0 0.0008 0 0.0001 0.0036 0.0011 0.0001 0.0004 0 0.0008 0.0002 0.0001 0.0002
Nucleus 0.0097 0.0011 0.0013 0.0014 0.0015 0.0008 0.0039 0.001 0.0015 0.005 0.0018 0.0013 0.0049 0.0009 0.002 0.0019 0.0009 0.0017
Peroxisomes 0.0071 0.0009 0.0017 0.0001 0.0002 0.0063 0.0084 0.0001 0.0027 0.0115 0.0009 0.0012 0.0088 0.0003 0.0035 0.0008 0.0112 0.0008
Punctate Nuclear 0.0062 0.001 0.0006 0.0001 0.0001 0.0007 0.0067 0.0001 0.0002 0.0039 0.0003 0.0002 0.0049 0.0001 0.0046 0.0008 0.0015 0.0027
Vacuole 0.0206 0.0062 0.018 0.0574 0.0435 0.0065 0.0352 0.0116 0.027 0.092 0.0308 0.0105 0.0215 0.0059 0.021 0.0289 0.0154 0.008
Vacuole Periphery 0.0078 0.0015 0.008 0.0209 0.0265 0.0048 0.0092 0.0015 0.0092 0.0443 0.0119 0.0063 0.011 0.0015 0.0098 0.0174 0.0112 0.006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 73.6566 136.8532 107.9379 81.182 78.6462 74.097 134.6735 116.8867 100.4123 80.0594
Translational Efficiency 1.4828 1.7185 1.5717 1.9898 1.8655 1.333 1.4528 1.7261 1.615 1.5981

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1655 292 882 659 1326 1657 164 830 2981 1949 1046 1489

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1465.63 1696.37 2332.48 2134.03 1531.59 1577.69 2453.46 2436.78 1494.97 1595.47 2351.45 2302.79
Standard Deviation 246.50 288.77 384.85 420.24 256.77 269.01 403.62 420.95 253.25 275.34 390.34 446.71
Intensity Change Log 2 0.210930 0.670344 0.542060 0.042784 0.679788 0.669946 0.127456 0.675177 0.608825

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001371 0.002200 0.002529 0.002903 0.001315 0.001841 0.003014 0.002503 0.001346 0.001895 0.002605 0.002680
Bud Neck 0.000628 0.001491 0.008759 0.004028 0.000707 0.001299 0.012480 0.003630 0.000663 0.001328 0.009343 0.003806
Bud Site 0.091767 0.119174* 0.102485* 0.141146* 0.075697 0.123981* 0.114859* 0.114220* 0.084619 0.123261* 0.104425* 0.126137*
Cell Periphery 0.003564 0.006903 0.008186 0.038837 0.004177 0.002694 0.012734 0.023821 0.003836 0.003325 0.008899 0.030467
Cytoplasm 0.415953* 0.317586* 0.188373* 0.371139* 0.454845* 0.450130* 0.185348* 0.442089* 0.433253* 0.430272* 0.187898* 0.410688*
Cytoplasmic Foci 0.267960* 0.235986* 0.071973 0.047161 0.208401* 0.208170* 0.103153 0.058777 0.241467* 0.212337* 0.076861 0.053636
Eisosomes 0.000015 0.000023 0.000020 0.000023 0.000012 0.000014 0.000023 0.000019 0.000013 0.000015 0.000021 0.000021
Endoplasmic Reticulum 0.020745 0.013848 0.006804 0.003961 0.020979 0.010730 0.008521 0.002124 0.020849 0.011197 0.007073 0.002937
Endosome 0.015201 0.024975 0.031581 0.013169 0.014554 0.019984 0.041307 0.010435 0.014913 0.020732 0.033106 0.011645
Golgi 0.098653 0.192675* 0.194932* 0.109440 0.106844 0.102354 0.148293* 0.128584* 0.102297 0.115886* 0.187619* 0.120111*
Lipid Particles 0.003596 0.011099 0.018771 0.063568 0.004213 0.002849 0.045359 0.042886 0.003870 0.004085 0.022940 0.052039
Mitochondria 0.001809 0.001193 0.000575 0.000435 0.001281 0.001310 0.000145 0.000329 0.001574 0.001293 0.000507 0.000376
Mitotic Spindle 0.002685 0.005375 0.025732 0.006446 0.004001 0.007357 0.028732 0.005491 0.003271 0.007060 0.026202 0.005914
None 0.000598 0.000487 0.000476 0.000467 0.000577 0.000415 0.000250 0.000390 0.000589 0.000426 0.000440 0.000424
Nuclear Periphery 0.000058 0.000068 0.000883 0.000194 0.000084 0.000064 0.000970 0.000188 0.000070 0.000064 0.000897 0.000191
Nuclear Periphery Foci 0.002195 0.003142 0.018048 0.008902 0.003472 0.001699 0.018978 0.006116 0.002763 0.001915 0.018193 0.007349
Nucleolus 0.000038 0.000085 0.000120 0.000101 0.000030 0.000039 0.000106 0.000098 0.000034 0.000046 0.000118 0.000099
Nucleus 0.000830 0.000684 0.001436 0.002007 0.000669 0.001107 0.003560 0.002193 0.000758 0.001043 0.001769 0.002111
Peroxisomes 0.000368 0.000737 0.000725 0.000789 0.000306 0.000818 0.001017 0.000741 0.000340 0.000806 0.000770 0.000762
Vacuole 0.016269 0.022577 0.216140* 0.138598 0.021678 0.023436 0.229708* 0.115070 0.018675 0.023307 0.218267* 0.125483
Vacuole Periphery 0.055696 0.039692 0.101454* 0.046686 0.076159 0.039708 0.041442 0.040297 0.064798 0.039705 0.092045 0.043125

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.09 -2.43 -4.03 -1.62 -0.60 -1.63 -2.09 -1.68 -0.94 0.54 -2.25 -2.97 -3.24 -1.86 -0.02
Bud Neck -3.32 -12.87 -13.09 -7.52 7.23 -3.98 -7.83 -12.28 -9.09 5.76 -5.15 -14.86 -17.87 -12.36 9.23
Bud Site -2.43 -2.26 -5.87 -1.84 -3.66 -7.78 -3.12 -5.59 1.00 0.13 -7.50 -4.10 -7.76 -0.79 -2.64
Cell Periphery -2.50 -6.34 -10.71 -8.94 -9.17 3.22 -4.42 -9.61 -10.40 -4.08 1.32 -7.41 -14.34 -14.48 -11.08
Cytoplasm 4.80 19.31 2.79 -2.50 -12.02 0.39 12.40 0.70 0.39 -11.31 0.31 24.63 1.89 1.49 -19.25
Cytoplasmic Foci 2.33 28.90 33.12 14.71 5.67 0.05 9.04 23.58 25.82 4.54 4.73 31.17 40.18 31.11 6.29
Eisosomes -2.07 -3.34 -4.35 -0.19 -1.06 -1.58 -3.70 -2.51 -1.81 1.10 -1.58 -4.86 -4.22 -3.02 0.17
Endoplasmic Reticulum 2.27 8.00 10.78 3.66 2.46 5.51 5.18 11.59 9.24 3.01 6.90 10.04 16.05 9.12 4.32
Endosome -5.10 -12.38 2.31 6.03 13.15 -5.76 -7.92 5.62 10.40 9.29 -7.18 -15.01 5.77 11.04 17.63
Golgi -7.55 -11.78 -1.05 6.52 9.01 0.97 -2.60 -2.15 -2.89 1.24 -3.33 -11.81 -2.57 0.17 8.54
Lipid Particles -3.31 -10.39 -12.54 -9.85 -8.87 2.74 -6.44 -13.33 -13.89 0.25 -0.46 -11.89 -18.02 -17.79 -9.28
Mitochondria 0.69 4.31 4.95 0.95 1.86 -0.15 7.51 5.94 6.05 -2.17 1.16 5.96 7.06 5.07 2.22
Mitotic Spindle -3.26 -10.04 -2.94 -0.86 7.57 -3.83 -3.08 -1.32 1.31 2.84 -5.56 -10.08 -3.12 0.94 8.50
None 0.38 0.67 0.66 0.06 -0.00 2.61 5.48 3.54 0.57 -3.35 1.44 1.38 1.53 -0.04 0.44
Nuclear Periphery -0.49 -12.33 -10.06 -6.09 10.32 1.37 -1.93 -4.14 -4.83 1.69 0.39 -9.36 -7.95 -7.58 7.92
Nuclear Periphery Foci -1.70 -11.94 -10.12 -7.64 6.49 5.03 -4.47 -5.27 -10.60 3.66 3.22 -12.51 -11.29 -14.69 8.59
Nucleolus -0.72 -5.84 -4.95 -0.22 2.38 -1.62 -4.50 -10.29 -7.61 0.63 -1.02 -8.06 -8.58 -4.57 2.57
Nucleus 0.55 -3.63 -3.07 -3.34 -1.75 -1.60 -4.04 -7.77 -3.57 1.70 -1.20 -6.19 -5.86 -3.92 -1.69
Peroxisomes -3.78 -2.93 -4.78 -0.22 -0.37 -3.14 -3.31 -6.03 0.57 1.23 -3.36 -4.00 -7.56 0.48 0.11
Vacuole -2.12 -26.48 -19.17 -16.86 7.84 -0.90 -11.66 -17.11 -16.76 5.86 -3.00 -28.73 -25.73 -23.96 11.23
Vacuole Periphery 3.13 -8.55 2.89 -0.99 10.28 9.14 6.90 9.02 0.35 0.08 9.33 -5.45 8.61 -0.72 11.52
Standard name
Human Ortholog
Description RNA binding protein involved in asymmetric localization of ASH1 mRNA; represses translation of ASH1 mRNA, an effect reversed by Yck1p-dependent phosphoryation; regulates telomere position effect and length; similarity to hnRNP-K
Localization
Cell Percentages cytoplasm (89%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-5

Hek2

Hek2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hek2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available