Standard name
Human Ortholog
Description High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.15 0.19 0.29 0.2 0.13 0.11 0.1 0.11 – 0.12 0.09 0.14 0.22 0.21 0.14 0.21 0.11 0.08 0 0 0 0 0 0
Bud 0.05 0.05 0 0.05 0.05 0.06 0.06 0.06 – 0.1 0.12 0.08 0 0 0 0.06 0.13 0.07 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.85 0.86 0.9 0.73 0.42 0.34 0.35 0.32 – 0.37 0.36 0.41 0.83 0.83 0.77 0.72 0.55 0.72 0.74 0.79 0.86 0.82 0.82 0.82
Cytoplasm 0.22 0.21 0.08 0.22 0.32 0.44 0.47 0.52 – 0.6 0.61 0.64 0.13 0.13 0.13 0.24 0.4 0.32 0.08 0 0 0 0 0.05
Endoplasmic Reticulum 0 0 0 0.15 0.28 0.2 0.13 0.13 – 0.09 0.05 0.06 0.09 0.1 0.13 0 0 0 0.15 0.09 0.08 0 0.08 0.05
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0 0 0 0 0.06 0.07 0
Nucleus 0 0 0 0 0 0.06 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.07 0.08 0.12 0.12 – 0.09 0.1 0.08 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 32 43 104 50 34 32 39 52 – 45 29 47 51 49 32 98 25 26 3 5 7 1 0 0
Bud 10 12 10 12 13 18 24 26 – 35 39 26 2 2 1 26 29 21 0 5 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 184 193 326 186 108 100 134 149 – 135 119 138 196 196 181 336 127 222 170 229 359 31 22 32
Cytoplasm 48 47 30 56 83 132 180 244 – 219 203 214 30 30 30 114 92 98 18 12 4 0 0 2
Endoplasmic Reticulum 8 0 14 39 72 61 49 60 – 32 17 19 21 23 30 12 6 10 35 25 33 0 2 1
Endosome 0 0 0 0 1 2 5 1 – 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 1 0 3 1 1 0 1 0 – 0 0 0 1 2 4 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 1 1 0 3 2 – 3 4 5 0 0 0 25 5 1 0 4 6 2 1 1
Nucleus 0 0 0 1 3 17 13 15 – 5 7 4 2 2 5 3 5 1 0 0 0 0 0 0
Nuclear Periphery 0 0 1 0 0 1 2 3 – 2 0 0 0 0 0 4 2 1 0 0 0 0 0 0
Nucleolus 0 0 0 0 1 0 1 1 – 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 1 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 2 1 3 2 – 0 0 2 1 1 3 1 1 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 1 0 19 25 44 55 – 32 34 28 8 8 6 7 8 2 0 1 4 0 0 0
Unique Cell Count 217 224 362 256 256 298 382 466 367 334 333 236 237 236 468 230 307 231 291 420 38 27 39
Labelled Cell Count 283 295 489 346 338 389 498 611 508 453 485 312 313 292 627 301 382 231 291 420 38 27 39


Bud, Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 39.0 32.3 27.9 23.9 18.0 15.6 15.9 16.2 – 15.2 15.0 15.1 20.0 20.0 18.3 23.1 24.9 29.7 – – –
Std Deviation (1e-4) 7.5 8.9 6.0 5.3 5.4 5.7 5.8 5.4 – 5.0 5.0 4.3 4.4 4.4 4.3 7.3 8.1 9.1 – – –
Intensity Change (Log2) – – – -0.22 -0.63 -0.83 -0.81 -0.79 – -0.88 -0.9 -0.88 -0.48 -0.48 -0.61 -0.27 -0.16 0.09 – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -2.6 -4.5 -5.7 -6.4 -6.4 – -5.5 -6.7 -4.7 -1.9 -2.2 -4.3 -2.6 -5.1 -6.6
Bud 1.3 1.5 2.1 2.3 2.0 – 3.8 4.6 3.0 0 0 0 2.0 4.7 2.5
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery -5.7 -12.8 -15.1 -15.4 -16.8 – -14.9 -14.9 -13.6 -2.5 -2.6 -4.4 -6.5 -9.7 -5.9
Cytoplasm 4.8 7.6 10.7 11.8 13.4 – 14.6 14.7 15.4 1.8 1.7 1.8 6.1 9.3 7.7
Endoplasmic Reticulum 5.0 8.6 6.7 4.4 4.5 – 2.7 0.8 1.1 2.6 2.9 4.0 -1.1 -0.8 -0.4
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 – 0 0 0 0 0 0 4.5 0 0
Nucleus 0 0 4.6 3.5 3.4 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 0 4.9 5.3 6.4 6.6 – 5.5 6.0 5.4 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 15.5205 23.5 21.3044 18.5614 18.8662 21.5266 18.7984 26.7036 23.3851 24.0666 21.1587 24.7886 22.6535 31.1895 27.3014 26.4663 25.7161 27.4968
Actin 0.0225 0.0006 0.0145 0.0068 0.0054 0.0028 0.0136 0.0003 0.0001 0 0.0039 0.005 0 0 0.0001 0 0.0001 0
Bud 0.0035 0.0003 0.0024 0.0068 0.0005 0.0033 0.0002 0.0001 0.0003 0.0001 0.0025 0.0004 0 0 0.0001 0.0001 0.0001 0.0001
Bud Neck 0.0009 0.0034 0.0037 0.0076 0.0029 0.0012 0.0012 0.0004 0.0007 0.0003 0.005 0.0008 0.0001 0.0003 0.0005 0.0003 0.0005 0.0008
Bud Periphery 0.0235 0.0021 0.0055 0.0548 0.0039 0.009 0.0015 0.0017 0.0071 0.0037 0.0195 0.0056 0.0012 0.0007 0.0015 0.0025 0.0026 0.0031
Bud Site 0.0044 0.0227 0.0205 0.0187 0.0016 0.0019 0.0029 0.0039 0.0128 0.0001 0.0097 0.0018 0.0004 0.0017 0.0011 0.0001 0.0001 0.0001
Cell Periphery 0.6468 0.7275 0.7491 0.6237 0.6357 0.7634 0.7982 0.8702 0.8647 0.8553 0.7617 0.8497 0.7815 0.8191 0.8458 0.7739 0.8057 0.8242
Cytoplasm 0.0287 0.0723 0.0387 0.0174 0.0451 0.029 0.0124 0.0335 0.0182 0.0023 0.0156 0.011 0.0076 0.0261 0.0118 0.0044 0.0063 0.0076
Cytoplasmic Foci 0.0088 0.0043 0.0022 0.0033 0.008 0.003 0.0007 0.0001 0.0001 0 0.0061 0.0019 0 0 0 0 0 0
Eisosomes 0.0136 0.0053 0.0016 0.0106 0.0089 0.0007 0.0033 0.0013 0.0038 0.0003 0.0025 0.001 0.0034 0.0013 0.001 0.0005 0.0007 0.0008
Endoplasmic Reticulum 0.0794 0.1076 0.0853 0.1661 0.1229 0.098 0.0869 0.055 0.0532 0.0762 0.0498 0.0807 0.1253 0.0932 0.0946 0.1677 0.1477 0.1056
Endosome 0.0196 0.0037 0.0048 0.0013 0.0414 0.0094 0.0009 0.0001 0.0001 0 0.0037 0.0006 0 0 0 0 0 0
Golgi 0.0168 0.0018 0.0028 0.001 0.0109 0.0053 0.0023 0.0002 0.0001 0 0.0017 0.0003 0 0 0 0 0.0001 0
Lipid Particles 0.0242 0.0086 0.0072 0.0061 0.0089 0.0124 0.0052 0.0035 0.0022 0.0009 0.02 0.002 0.0093 0.0044 0.0021 0.0037 0.0027 0.0079
Mitochondria 0.0108 0.0029 0.0167 0.0097 0.0184 0.0092 0.002 0 0.0007 0 0.0047 0.0023 0 0 0 0 0 0
None 0.0121 0.0003 0.0006 0.0113 0.0026 0.0007 0.0014 0.0002 0.0064 0 0.0569 0.0047 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001
Nuclear Periphery 0.0152 0.0076 0.0064 0.0064 0.0174 0.0079 0.0121 0.0049 0.0044 0.0039 0.0037 0.0038 0.0147 0.0133 0.0092 0.0079 0.0069 0.0054
Nucleolus 0.0008 0.0005 0.0007 0.0028 0.008 0.0009 0.0002 0.0001 0.0001 0 0.0023 0.0002 0.0001 0 0 0.0002 0 0
Nucleus 0.0037 0.0005 0.0008 0.0032 0.0101 0.0008 0.0092 0.0002 0.0002 0.0001 0.0014 0.0004 0.0001 0.0002 0.0001 0.0002 0.0001 0.0001
Peroxisomes 0.0066 0.0001 0.0015 0.0003 0.002 0.0008 0.0024 0 0 0 0.0015 0.0002 0 0 0 0 0 0
Punctate Nuclear 0.0028 0.0003 0.0002 0.0015 0.005 0.0003 0.0003 0 0 0 0.0007 0.0004 0 0 0 0 0 0
Vacuole 0.0534 0.0274 0.0342 0.0393 0.0378 0.0386 0.0427 0.0241 0.0246 0.0563 0.0256 0.0269 0.0556 0.0393 0.0318 0.038 0.026 0.0439
Vacuole Periphery 0.002 0.0002 0.0006 0.0015 0.0024 0.0013 0.0003 0.0001 0.0002 0.0004 0.0013 0.0003 0.0002 0.0001 0.0001 0.0002 0.0003 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 31.8953 24.1457 44.9834 65.2613 29.0593 14.3593 19.8686 20.5093 36.6922 39.5666
Translational Efficiency 0.9243 0.8626 0.932 0.7497 1.0435 1.5318 0.7364 0.6892 0.7122 0.7001

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress
Localization
Cell Percentages cell periphery (95%)
Cell Cycle Regulation No
Subcompartmental Group cell periphery-1

Fui1

Fui1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fui1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available