Standard name
Human Ortholog
Description Lipid raft associated protein; interacts with the plasma membrane ATPase Pma1p and has a role in its targeting to the plasma membrane by influencing its incorporation into lipid rafts; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST1 has a paralog, AST2, that arose from the whole genome duplication
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.84 0.87 0.92 0.84 0.93 0.86 0.84 0.89 0.75 0.82 0.71 0.7 0.93 0.95 0.97 0.74 0.64 0.68 0.69 0.77 0.84 0.69 0.79 0.72
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0.07 0.16 0.18 0.34 0.31 0.18 0.44 0.3 0.51 0.48 0 0 0 0.09 0.05 0 0 0 0 0 0 0.08
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.15 0.07 0.07 0 0.06 0 0.05 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.12 0.15 0.05 0.16 0 0 0 0 0 0 0 0 0.07 0.07 0 0.18 0.32 0.29 0.15 0.06 0 0.15 0.08 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 5 3 2 3 8
Bud 0 1 0 1 9 15 13 6 25 19 37 38 0 0 0 0 0 0 2 5 0 4 3 13
Bud Neck 0 0 0 0 2 3 4 3 1 3 4 7 1 1 2 0 0 0 1 3 0 1 1 2
Bud Site 0 0 0 0 0 2 2 0 5 3 6 6 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 2 3 2 3 6 3 7 6 6 0 1 1 0 1 0 0 0 0 0 1 1
Cytoplasm 131 384 202 294 742 784 1010 833 637 815 668 860 225 595 630 87 75 62 140 378 201 250 349 378
Endoplasmic Reticulum 0 2 2 0 0 1 1 0 0 0 0 3 3 3 0 4 5 0 0 1 2 2 2 8
Endosome 3 5 0 6 0 1 2 4 1 0 2 1 1 0 3 3 4 2 2 9 2 1 4 14
Golgi 0 1 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 1 1 2 1 0 2
Mitochondria 8 2 16 55 141 307 371 166 369 294 473 590 1 0 0 11 6 0 6 6 3 13 15 41
Nucleus 3 7 2 3 1 5 4 9 5 2 4 6 8 5 5 17 8 6 8 31 6 16 14 1
Nuclear Periphery 2 1 1 0 1 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 1 2 1 1 2 2 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 2 0 0 1 1 1 1 0 0 0 0 0 0 0 0 1 0 3
SpindlePole 0 0 0 1 3 4 10 20 11 23 21 28 0 0 0 1 1 0 2 7 4 3 5 21
Vac/Vac Membrane 18 68 11 55 12 3 2 3 13 5 5 2 18 47 22 21 37 26 30 29 7 53 37 22
Unique Cell Count 156 443 220 351 799 913 1201 933 848 994 935 1221 243 629 650 117 117 91 202 489 241 363 445 527
Labelled Cell Count 165 472 234 417 915 1127 1425 1052 1071 1173 1231 1551 259 652 664 145 137 96 202 489 241 363 445 527


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 5.7 4.2 4.1 3.4 3.1 2.8 3.2 2.8 2.7 2.6 2.6 5.9 5.6 5.4 5.8 6.2 7.0 6.4 6.1 5.8
Std Deviation (1e-4) 0.9 1.0 0.8 1.2 1.3 1.5 1.3 1.4 1.2 0.8 1.1 0.6 1.1 1.0 1.0 1.0 1.1 1.9 1.2 1.4 1.3
Intensity Change (Log2) – – – -0.02 -0.31 -0.42 -0.6 -0.38 -0.58 -0.63 -0.69 -0.71 0.49 0.42 0.37 0.48 0.56 0.73 0.62 0.55 0.46


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -2.8 0.5 -2.4 -3.0 -1.1 -5.4 -3.6 -6.3 -6.6 0.3 1.5 3.2 -4.4 -6.3 -5.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 -3.9 0 0 0.7 -0.8 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 5.2 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 3.9 0 0 0 0 0 0 0 0 1.1 1.3 -1.1 3.9 6.7 5.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.2726 1.6803 1.3504 1.0846 1.4316 1.445 1.6188 2.1293 2.0922 1.345 1.853 1.8934 -0.8814 0.3071 -0.1855 0.2681 -0.1915 0.1784
Actin 0.0346 0.0048 0.005 0.0075 0.0047 0.0063 0.0388 0.0008 0.0159 0.0017 0.0184 0.0016 0.0125 0.0003 0.0074 0.0198 0.0414 0.0024
Bud 0.0055 0.0154 0.017 0.0094 0.0196 0.0054 0.0014 0.0007 0.0004 0.001 0.0005 0.0004 0.0002 0.0002 0.0004 0.0004 0.0006 0.0002
Bud Neck 0.0058 0.0008 0.0024 0.0009 0.0073 0.0045 0.0026 0.0005 0.0011 0.0006 0.0012 0.0014 0.0032 0.0087 0.0006 0.0011 0.0021 0.0027
Bud Periphery 0.0044 0.0041 0.0038 0.0129 0.0046 0.0041 0.002 0.0013 0.0003 0.0024 0.0006 0.0004 0.0002 0.0004 0.0006 0.0003 0.0005 0.0001
Bud Site 0.0109 0.0279 0.019 0.008 0.0351 0.0031 0.0127 0.0056 0.0091 0.0007 0.0082 0.0004 0.0066 0.0024 0.0014 0.0013 0.0023 0.0002
Cell Periphery 0.0007 0.0005 0.0008 0.0001 0.0006 0.0003 0.0004 0.0004 0.0002 0.0001 0.0001 0.0001 0.0001 0.0004 0.0001 0 0.0001 0
Cytoplasm 0.2426 0.4242 0.4183 0.4515 0.2754 0.5113 0.4169 0.6149 0.5822 0.5421 0.4411 0.5977 0.5127 0.6183 0.5853 0.4753 0.5576 0.7186
Cytoplasmic Foci 0.0645 0.0253 0.0281 0.0562 0.039 0.0514 0.0258 0.0081 0.0162 0.0235 0.0268 0.014 0.0192 0.0096 0.01 0.0257 0.0209 0.0064
Eisosomes 0.001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0003 0.0001 0.0002 0.0001 0.0002 0.0001 0.0002 0 0.0001 0.0001 0.0004 0
Endoplasmic Reticulum 0.0059 0.0031 0.0029 0.0015 0.004 0.0043 0.0074 0.003 0.0053 0.0056 0.0033 0.0044 0.0064 0.0037 0.0047 0.0072 0.0038 0.004
Endosome 0.0508 0.0075 0.0203 0.0099 0.0225 0.0157 0.0244 0.0048 0.012 0.0114 0.0236 0.01 0.0124 0.0026 0.0145 0.0154 0.0135 0.0102
Golgi 0.0177 0.0024 0.01 0.0096 0.0071 0.0061 0.0069 0.001 0.004 0.0039 0.0083 0.0024 0.0033 0.0002 0.0033 0.0045 0.0062 0.0007
Lipid Particles 0.0297 0.0069 0.0104 0.0067 0.0896 0.0078 0.01 0.0013 0.0018 0.0125 0.0102 0.0021 0.0094 0.0005 0.007 0.0064 0.0227 0.0119
Mitochondria 0.0192 0.0065 0.0179 0.0176 0.0104 0.0228 0.007 0.0014 0.0054 0.0072 0.0043 0.0079 0.0011 0.0002 0.0023 0.002 0.0029 0.0008
None 0.3954 0.3463 0.3405 0.2218 0.3283 0.2357 0.3352 0.288 0.2747 0.2861 0.3969 0.2819 0.3812 0.2995 0.296 0.2478 0.1912 0.0962
Nuclear Periphery 0.0159 0.005 0.0068 0.004 0.0051 0.0105 0.0116 0.0022 0.0057 0.0082 0.0045 0.0042 0.0044 0.0033 0.0053 0.0158 0.0062 0.0078
Nucleolus 0.0026 0.0027 0.0035 0.0039 0.002 0.0035 0.0053 0.0023 0.0005 0.0005 0.0005 0.0003 0.0006 0.0003 0.0003 0.0006 0.0009 0.0006
Nucleus 0.0225 0.0743 0.0524 0.0679 0.0186 0.0533 0.0504 0.0419 0.0541 0.0716 0.013 0.0525 0.0119 0.0435 0.0467 0.085 0.1034 0.1247
Peroxisomes 0.0366 0.0066 0.0126 0.0583 0.0367 0.009 0.0224 0.0005 0.0027 0.0109 0.0254 0.0096 0.007 0.0006 0.0073 0.0733 0.0086 0.0008
Punctate Nuclear 0.0216 0.0333 0.024 0.0499 0.0872 0.0414 0.0092 0.0166 0.0055 0.0068 0.008 0.0068 0.0047 0.0037 0.0035 0.0156 0.0127 0.0092
Vacuole 0.0096 0.0021 0.003 0.0016 0.0016 0.0023 0.0074 0.0041 0.0022 0.0022 0.0043 0.0016 0.0022 0.0016 0.0024 0.002 0.0014 0.002
Vacuole Periphery 0.0026 0.0003 0.0011 0.0006 0.0005 0.0012 0.002 0.0005 0.0006 0.0009 0.0005 0.0003 0.0003 0.0001 0.0009 0.0006 0.0005 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.6794 28.2914 24.9652 23.5533 21.071 21.372 33.6307 29.4819 29.1444 24.1016
Translational Efficiency 2.1917 2.0781 1.5707 1.5465 1.7228 1.6556 1.6775 1.6352 1.305 1.5344

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Lipid raft associated protein; interacts with the plasma membrane ATPase Pma1p and has a role in its targeting to the plasma membrane by influencing its incorporation into lipid rafts; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST1 has a paralog, AST2, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (51%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Ast1

Ast1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ast1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available