Standard name
Human Ortholog
Description Cytoplasmic isoleucine-tRNA synthetase; target of the G1-specific inhibitor reveromycin A
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0.06 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0.06 0.05 0.07 0.08 – 0.11 0.12 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 1.0 0.98 0.99 0.96 0.9 0.87 0.78 0.8 – 0.78 0.71 0.99 0.99 0.98 0.99 1.0 0.97 0.98 0.98 0.97 0.95 0.94 0.91
Endoplasmic Reticulum 0 0 0 0 0 0.07 0.12 0.18 0.14 – 0.12 0.14 0 0 0 0.05 0 0 0 0 0 0 0 0.05
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 – 0.05 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 3 6 8 14 – 18 39 0 0 0 0 0 0 0 0 0 0 0 2
Bud 0 0 4 2 1 3 2 5 2 – 2 3 0 1 0 0 0 1 0 2 0 0 1 4
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 5 7 8 27 23 36 32 – 32 55 0 2 3 2 3 0 0 0 0 0 0 0
Cytoplasm 203 234 304 298 320 442 434 423 339 – 235 316 297 473 487 156 100 109 192 230 306 267 369 358
Endoplasmic Reticulum 1 0 5 3 12 36 61 95 58 – 35 63 4 6 15 8 4 4 0 0 1 3 11 18
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 4 2 – 2 3 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 1 0 1 2 4 10 – 14 32 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 1 1 – 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Nuclear Periphery 0 0 0 0 0 0 0 1 0 – 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 2 1 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 1 2 2 – 1 4 0 0 0 1 0 0 0 1 2 4 3 1
Unique Cell Count 204 234 309 302 333 490 501 541 425 302 446 300 478 498 158 100 112 197 236 317 282 393 392
Labelled Cell Count 204 235 318 311 342 512 531 580 460 339 516 301 482 505 167 107 115 197 236 317 282 393 392


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 129.9 105.9 73.0 71.1 59.5 49.9 43.6 38.2 35.1 – 31.1 29.1 102.7 95.7 82.0 116.9 139.1 132.3 107.9 106.1 105.5
Std Deviation (1e-4) 23.3 24.2 13.8 12.8 12.4 9.9 9.5 9.0 8.3 – 7.7 7.6 17.5 18.0 16.5 31.1 27.7 40.6 18.5 24.4 26.4
Intensity Change (Log2) – – – -0.04 -0.29 -0.55 -0.74 -0.93 -1.06 – -1.23 -1.33 0.49 0.39 0.17 0.68 0.93 0.86 0.56 0.54 0.53


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 3.2 – 4.4 5.3 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0.6 0.7 2.7 2.2 3.3 3.6 – 4.7 5.4 0 0 0 0 0 0
Cytoplasm 0.3 -1.8 -4.5 -5.7 -8.0 -7.6 – -7.9 -9.7 0.7 0.7 -0.6 0.3 1.3 -0.7
Endoplasmic Reticulum 0 1.6 3.6 5.3 6.9 5.7 – 5.0 5.9 0 0 1.2 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 3.8 4.8 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 78.6297 120.1029 105.6362 100.471 58.6987 84.5754 94.4717 127.8152 113.479 108.0631 112.0743 103.8925 81.4868 109.4859 95.9629 88.2252 99.9726 70.5344
Actin 0.0328 0.0002 0.0018 0.0019 0.0037 0.0016 0.004 0.0001 0.0021 0.003 0.0002 0.0003 0.0181 0.0002 0.0048 0.0005 0.0003 0.0667
Bud 0.0025 0.0005 0.0011 0.0009 0.0011 0.0016 0.0009 0.0002 0.0011 0.0035 0.0006 0.0003 0.0017 0.0002 0.0016 0.0007 0.0003 0.0006
Bud Neck 0.0015 0.0008 0.0014 0.0007 0.0011 0.0025 0.0004 0.0004 0.0005 0.0006 0.0005 0.001 0.0005 0.0002 0.0006 0.0005 0.0019 0.0004
Bud Periphery 0.003 0.0005 0.0015 0.0007 0.0023 0.0014 0.0008 0.0001 0.0008 0.0055 0.0002 0.0001 0.003 0.0001 0.0014 0.0003 0.0001 0.0014
Bud Site 0.0031 0.0005 0.0004 0.0017 0.0007 0.001 0.0015 0.0001 0.0012 0.0006 0.0003 0.0001 0.0008 0.0002 0.0041 0.0002 0 0.0004
Cell Periphery 0.0286 0.0189 0.0242 0.0175 0.0085 0.0036 0.01 0.0131 0.0137 0.0112 0.0182 0.001 0.0018 0.0013 0.0035 0.002 0.0005 0.0002
Cytoplasm 0.6062 0.8472 0.7712 0.7615 0.4776 0.5315 0.7935 0.958 0.8655 0.8284 0.8931 0.8996 0.8301 0.9855 0.8956 0.8781 0.8862 0.7028
Cytoplasmic Foci 0.0153 0.0009 0.002 0.0011 0.0093 0.0077 0.0117 0.0004 0.004 0.0012 0.0066 0.0042 0.0083 0.0007 0.004 0.0019 0.0029 0.0117
Eisosomes 0.0003 0 0 0.0001 0.0015 0.0001 0.001 0 0.0005 0.0001 0 0 0.0001 0 0.0001 0.0001 0 0.0009
Endoplasmic Reticulum 0.0669 0.0295 0.0252 0.038 0.012 0.0857 0.0204 0.0033 0.0116 0.0235 0.0025 0.0574 0.0374 0.0024 0.0083 0.0025 0.0124 0.0121
Endosome 0.0125 0.0013 0.0032 0.0039 0.0216 0.0675 0.0043 0.0004 0.005 0.0165 0.0117 0.0068 0.013 0.0009 0.0082 0.0054 0.0107 0.0649
Golgi 0.0053 0.0002 0.0011 0.0005 0.0083 0.0063 0.0013 0 0.0008 0.0047 0.0011 0.0009 0.003 0.0001 0.0037 0.0003 0.0042 0.032
Lipid Particles 0.0092 0.0008 0.0055 0.0018 0.0461 0.0017 0.0079 0.0013 0.0028 0.001 0.003 0.001 0.002 0.0005 0.0011 0.0013 0.0001 0.0222
Mitochondria 0.0097 0.0018 0.0033 0.0006 0.1152 0.0109 0.0046 0 0.009 0.0143 0.002 0.0004 0.0128 0.0001 0.0127 0.0402 0.0016 0.0216
None 0.0024 0.0002 0.0006 0.0007 0.0035 0.0033 0.0093 0.0001 0.0057 0.0004 0.001 0.001 0.005 0.0001 0.0011 0.001 0.0001 0.0154
Nuclear Periphery 0.0293 0.01 0.0089 0.0093 0.0186 0.0202 0.0266 0.0017 0.0128 0.0087 0.0031 0.0043 0.0167 0.0007 0.0027 0.0035 0.0014 0.0163
Nucleolus 0.0021 0.0027 0.0023 0.0009 0.0219 0.0313 0.0086 0 0.006 0.0003 0.0006 0.0002 0.0011 0 0.0002 0.0043 0 0.0003
Nucleus 0.0076 0.0032 0.0041 0.0049 0.0179 0.0656 0.022 0.0012 0.0077 0.0046 0.0027 0.0039 0.0043 0.0006 0.0019 0.0047 0.0008 0.0013
Peroxisomes 0.0027 0.0001 0.0018 0 0.0038 0.0003 0.0006 0 0.0004 0 0.0001 0 0.002 0 0.0018 0.0001 0 0.014
Punctate Nuclear 0.0087 0.0001 0.0001 0.0001 0.0006 0.0379 0.0052 0 0.0007 0.0001 0.0003 0.0003 0.0018 0 0.0002 0.0001 0 0.0032
Vacuole 0.1238 0.075 0.1176 0.1358 0.1314 0.0784 0.0557 0.0189 0.0429 0.0617 0.0458 0.0142 0.0285 0.0057 0.0351 0.0314 0.0569 0.0042
Vacuole Periphery 0.0264 0.0057 0.0228 0.0176 0.0934 0.0398 0.0098 0.0005 0.0052 0.0103 0.0063 0.003 0.008 0.0005 0.0071 0.0209 0.0195 0.0076

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 484.689 440.0237 432.93 436.3434 411.0954 435.0879 425.1562 385.3905 404.5791 414.1546
Translational Efficiency 0.8316 0.8835 0.8848 0.9125 0.8762 0.7695 0.8417 0.7091 0.6987 0.7153

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
472 1290 176 979 1800 1401 250 77 2272 2691 426 1056

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 6124.75 7161.39 10438.86 6545.47 5715.82 6588.89 8516.39 7318.42 5800.77 6863.33 9310.65 6601.83
Standard Deviation 1174.21 1495.34 1764.20 1625.58 1449.48 1430.08 1154.99 1508.33 1406.58 1489.45 1721.87 1629.76
Intensity Change Log 2 — 0.225589 0.769241 0.095846 — 0.205075 0.575282 0.356572 — 0.215722 0.678859 0.227594

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000104 0.000082 0.000206 0.000591 0.000035 0.000086 0.000172 0.000673 0.000049 0.000084 0.000186 0.000597
Bud Neck 0.000632 0.000490 0.006981 0.003256 0.000182 0.000969 0.003258 0.002539 0.000276 0.000740 0.004796 0.003204
Bud Site 0.005315 0.002916 0.006434 0.011707 0.000923 0.002145 0.003979 0.009332 0.001836 0.002515 0.004993 0.011534
Cell Periphery 0.034718 0.015583 0.066417 0.016536 0.013347 0.012340 0.060427 0.131934* 0.017787 0.013895 0.062902 0.024951
Cytoplasm 0.717914* 0.827552* 0.330829* 0.545706* 0.847020* 0.812428* 0.495882* 0.425406* 0.820199* 0.819678* 0.427691* 0.536934*
Cytoplasmic Foci 0.014724 0.006253 0.034782 0.009239 0.003860 0.010400 0.007913 0.004763 0.006117 0.008412 0.019014 0.008913
Eisosomes 0.000003 0.000002 0.000012 0.000006 0.000001 0.000002 0.000008 0.000014 0.000001 0.000002 0.000010 0.000007
Endoplasmic Reticulum 0.000320 0.000480 0.000212 0.000613 0.000506 0.000296 0.000277 0.000115 0.000467 0.000384 0.000250 0.000576
Endosome 0.000659 0.000384 0.000718 0.003256 0.000241 0.000429 0.000418 0.000200 0.000328 0.000407 0.000542 0.003034
Golgi 0.066475 0.030293 0.051138 0.012447 0.030652 0.029701 0.018812 0.005189 0.038094 0.029985 0.032167 0.011918
Lipid Particles 0.019128 0.014289 0.042511 0.018475 0.008418 0.013755 0.047609 0.049061 0.010643 0.014011 0.045502 0.020705
Mitochondria 0.000107 0.000130 0.000240 0.000397 0.000111 0.000115 0.000077 0.000522 0.000110 0.000122 0.000144 0.000406
Mitotic Spindle 0.001270 0.000772 0.002427 0.001424 0.000356 0.000949 0.000860 0.000281 0.000546 0.000864 0.001508 0.001341
None 0.002913 0.003009 0.001823 0.002681 0.002934 0.002963 0.001199 0.000981 0.002930 0.002985 0.001457 0.002557
Nuclear Periphery 0.000628 0.000665 0.004836 0.000366 0.000487 0.000560 0.001613 0.000589 0.000517 0.000610 0.002944 0.000383
Nuclear Periphery Foci 0.010637 0.011463 0.022940 0.004689 0.009484 0.010402 0.012952 0.004366 0.009724 0.010910 0.017079 0.004665
Nucleolus 0.000060 0.000083 0.000152 0.000326 0.000042 0.000213 0.000098 0.000097 0.000046 0.000150 0.000120 0.000310
Nucleus 0.014279 0.012653 0.034472 0.020353 0.009030 0.021470 0.017722 0.012846 0.010120 0.017243 0.024642 0.019806
Peroxisomes 0.000018 0.000018 0.000016 0.000155 0.000014 0.000013 0.000018 0.000063 0.000015 0.000016 0.000017 0.000149
Vacuole 0.062884 0.048717 0.320275* 0.319850* 0.043719 0.062244 0.305623* 0.342461* 0.047700 0.055759 0.311676* 0.321499*
Vacuole Periphery 0.047213 0.024166 0.072578 0.027925 0.028637 0.018520 0.021084 0.008568 0.032496 0.021227 0.042359 0.026514

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.31 -4.07 -2.53 -2.57 -1.83 -8.61 -7.91 -1.50 -1.39 -1.21 -4.06 -9.48 -3.09 -2.83 -2.17
Bud Neck 1.12 -5.15 -8.44 -8.85 2.70 -7.42 -6.57 -3.63 -2.21 1.43 -7.60 -7.82 -9.82 -8.16 2.28
Bud Site 1.87 -0.37 -4.01 -6.70 -2.09 -6.69 -4.18 -2.28 -1.97 -1.54 -2.53 -2.84 -8.15 -7.49 -4.21
Cell Periphery 6.14 -5.05 6.64 1.07 9.47 0.62 -8.61 -6.49 -6.55 -3.77 3.00 -11.27 -1.96 -3.92 9.36
Cytoplasm -6.50 14.35 10.14 22.43 -8.26 4.82 15.89 11.98 10.61 1.23 0.92 23.01 25.19 25.01 -5.76
Cytoplasmic Foci 5.37 -4.19 2.85 -2.44 5.23 -9.94 -4.00 -0.96 2.20 1.42 -4.58 -6.17 -2.62 -0.25 4.59
Eisosomes 2.50 -7.67 -3.64 -4.60 4.12 -5.48 -7.59 -2.92 -2.64 -1.24 -4.23 -11.16 -5.67 -4.92 2.90
Endoplasmic Reticulum -1.43 2.35 -0.71 -0.03 -1.21 2.30 2.48 4.38 3.02 2.19 1.03 3.10 0.03 -0.37 -1.03
Endosome 2.78 -1.05 -2.94 -3.39 -2.75 -5.08 -4.40 0.09 3.28 3.12 -2.49 -5.33 -3.46 -3.31 -3.02
Golgi 7.31 3.10 11.92 9.99 10.58 0.59 5.74 14.27 13.52 7.59 4.21 3.01 15.72 13.78 10.32
Lipid Particles 1.81 -5.41 0.60 -1.67 6.21 -4.92 -8.22 -5.80 -4.89 -0.11 -3.52 -10.73 -6.02 -3.39 7.52
Mitochondria -0.70 -1.57 -3.56 -3.48 -1.50 -0.35 1.57 -0.90 -0.89 -0.93 -0.91 -1.18 -3.60 -3.46 -2.96
Mitotic Spindle 1.59 -2.19 -0.46 -1.57 1.67 -5.80 -4.69 0.57 5.28 4.39 -3.81 -4.03 -2.13 -1.22 0.58
None -0.29 3.15 0.65 0.78 -0.77 0.02 5.78 4.32 2.78 0.27 -0.09 5.77 0.87 0.87 -1.29
Nuclear Periphery -0.55 -8.21 2.98 3.22 8.61 -2.03 -6.67 -1.06 0.43 6.03 -2.34 -9.93 1.84 3.85 10.25
Nuclear Periphery Foci -0.78 -5.25 5.95 7.24 8.41 -1.36 -2.76 7.12 7.81 6.83 -1.91 -5.91 8.31 9.90 10.34
Nucleolus -0.72 -7.05 -8.06 -4.94 -4.71 -1.15 -4.61 -2.98 0.73 0.03 -1.35 -8.27 -8.62 -2.04 -5.78
Nucleus 0.82 -4.99 -6.11 -7.08 1.71 -8.07 -4.75 -1.42 2.65 1.97 -6.91 -7.15 -8.35 -4.67 0.03
Peroxisomes 0.02 0.04 -5.01 -5.02 -5.02 -0.38 -1.05 -3.79 -3.71 -3.19 -0.75 -1.17 -5.34 -5.26 -5.11
Vacuole 2.13 -13.59 -24.30 -27.46 -0.23 -5.31 -15.45 -11.10 -10.15 -0.79 -3.42 -20.95 -30.06 -28.57 -0.52
Vacuole Periphery 4.51 -2.57 4.77 0.45 6.75 5.11 3.32 9.86 5.30 5.05 5.89 -2.11 4.71 -0.48 5.15
Standard name
Human Ortholog
Description Cytoplasmic isoleucine-tRNA synthetase; target of the G1-specific inhibitor reveromycin A
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Ils1

Ils1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ils1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available